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Papaya

Engineering Resistance against Papaya ringspot virus by Native, Chimeric and Synthetic Transgenes

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Virus and Virus-like Diseases of Major Crops in Developing Countries

Abstract

The case of transgenic papayas resistant to Papaya ringspot virus (PRSV) is a paradigm in the control of a plant pathogen: from the conventional strategies to the more sophisticated use of recombinant DNA technologies, the engineered papayas have served as an example in the use of novel approaches to protect crop plants against plant viruses, and as a model system to transfer the technology to several different countries. PRSV-resistant transgenic papayas were the first commercialized transgenic fruit on a worldwide basis. It is also the only case in which the genetic engineering of the coat protein (CP) gene has led to the development of plants with varying degrees of resistance through the use of different entire CP genes in different programs and countries around the world.

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References

  • An, G., Ebert, P., Mitra, A., and Ha, S. 1988. Binary vectors In: Plant Molecular Biology Manual). S. Gelvin and Schilperoort, RA. Kluwer Academic Publishers, Boston

    Google Scholar 

  • Bateson, M., Henderson, J., Chaleeprom, W., Gibbs, A., and Dale, J. 1994. Papaya ringspot virus: isolate variability and the origin of PRSV type W (Aust). Arch. Virol. 75: 3547–3553.

    Google Scholar 

  • Bateson, M., Lines, R., Revill, P., Chaleeprom, W., Ha, C., Gibbs, A., and Dale, J. 2002. On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus. J. Gen. Virol. 83: 2575–2585.

    Google Scholar 

  • Bau, H., Cheng, Y., Yu, T., Yang, J., and Yeh, S. 2003. Broad-spectrum resistance to different geographic strains of Papaya ringspot virus in coat protein gene transgenic papaya. Phytopathology 93: 112–120.

    Google Scholar 

  • Baulcombe, D. 1996. Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8: 1833–1844.

    Google Scholar 

  • Bayot, R., Villegas, V., Magdalita, P., Jovellana, M., Espino, T., and Exconde, S. 1990. Seed transmissibility of Papaya ringspot virus. Phillip. J. Crop Sci. 15: 107–111.

    Google Scholar 

  • Beachy, R. 1990. Coat protein-mediated resistance against virus infection. Ann. Rev. PhytoPathol. 28: 451–474.

    Google Scholar 

  • Bhaskar, R. B. 1983. Confirmation of the etiology of Papaya mosaic virus. Indian J. Agric. Sci. 53: 479–481.

    Google Scholar 

  • Bindslev-Jensen, C. 1998. Allergy risks of genetically engineered foods. Allergy 53(Suppl. 45): 58–61.

    Google Scholar 

  • Brunt, A., Crabtree, K., Dallwitz, M., Gibbs, A., and Watson, L. 1996. Viruses of Plants. CAB International, UK.

    Google Scholar 

  • Cai, W., Gonsalves, C., Tennant, P., Fermin, G., Souza Jr., M., Sarindu, N., Jan, F.-J., Zhu, H.-Y., and Gonsalves, D. 1999. A protocol for efficient transformation and regeneration of Carica papaya L. In Vitro Cell. Dev.-Pl. 35: 61–69.

    Google Scholar 

  • Camarco-Rosa, F., Lima, J., and Pio-Ribeiro, G. 1998. Transmission and presence in the soil of Papaya lethal yellowing virus. Fitopatol. Bras. 23: 453–458.

    Google Scholar 

  • Carrington, J., and Whitham, S. 1998. Viral invasion and host defense: strategies and counterstrategies. Curr. Opin. Plant Biol. 1: 336–341.

    Google Scholar 

  • Chang, L.-S., Lee, Y.-S., Su, H.-J., and Hung, T.-H. 2003. First report of Papaya leaf curl virus infecting papaya plants in Taiwan. Plant Dis. 87: 204.

    Google Scholar 

  • Chen, G., Ye, C., Huang, J., Yu, M., and Li, B. 2001. Cloning of the Papaya ringspot virus (PRSV) replicase gene and generation of PRSV-resistant papayas through the introduction of the PRSV replicase gene. Plant Cell Rep. 20: 272–277.

    Google Scholar 

  • Chiang, C.-H., Wang, J.-J., Jan, F.-J., Yeh, S.-D., and Gonsalves, D. 2001. Comparative reactions of recombinant Papaya ringspot viruses with chimeric coat protein (CP) genes and wild-type viruses on CP-transgenic papaya. J. Gen. Virol. 82: 2827–2836.

    Google Scholar 

  • Davis, M., and Ying, Z. 2002. Development of transgenic ringspot virus-resistant papaya for Florida. Phytopathology 92:S18.

    Google Scholar 

  • Elbashir, S., Lendeckel, W., and Tuschl, T. 2001. RNA interference is mediated by 21-and 22-nucleotide RNAs. Gene. Dev. 15: 188–200.

    Google Scholar 

  • Fermin, G. 2002. Use, application, and technology transfer of native and synthetic genes to engineering single and multiple transgenic viral resistance. Department of Plant Pathology, Cornell University. 293.

    Google Scholar 

  • Fermin, G., and Gonsalves, D. 2003. Multiple virus resistance to three non-related tospoviruses by a single and short non-native transgene. Manuscript in preparation

    Google Scholar 

  • Fermin, G., Inglessis, V., Garboza, C., Rangel, S., Dagert, M., and Gonsalves, D. 2003. Technology transfer for engineered resistance against PRSV in Venezuelan transgenic papayas. Plant Dis. (accepted for publication).

    Google Scholar 

  • Ferreira, S., Pitz, K., Manshardt, R., Zee, F., Fitch, M., and Gonsalves, D. 2002. Virus coat protein transgenic papaya provides practical control of Papaya ringspot virus in Hawaii. Plant Dis. 86: 101–105.

    Google Scholar 

  • Fitch, M., Manshardt, R., Gonsalves, D., and Slightom, J. 1993. Transgenic papaya plants from Agrobacterium-mediated transformation of somatic embryos. Plant Cell Rep. 12: 245–249.

    Google Scholar 

  • Fitch, M., Manshardt, R., Gonsalves, D., Slightom, J., and Sanford, J. 1990. Stable transformation of papaya via microprojectile bombardment. Plant Cell Rep. 9: 189–194.

    Google Scholar 

  • Fitch, M., Manshardt, R., Gonsalves, D., Slightom, J., and Sanford, J. 1992. Virus resistant papaya derived from tissues bombarded with the coat protein gene of Papaya ringspot virus. Bio/Technol. 10: 1466–1472.

    Google Scholar 

  • Fuchs, M., and Gonsalves, D. 1995. Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of Zucchini yellow mosaic virus and Watermelon mosaic virus 2 to mixed infections by both potyviruses. Bio/Technol. 13: 1466–1473.

    Google Scholar 

  • Garzón-Tiznado, J., Acosta-García, G., Torres-Pacheco, I., González-Chavira, M., Rivera-Bustamante, R., Maya-Hernández, V., and Guevara-González, R. 2002. Presencia de los Geminivirus, Huasteco del Chile (PHV), Texano del Chile variante Tamaulipas (TPV-T), y Chino del Tomate (VCdT), en los estados de Guanajuato, Jalisco y San Luis Potosi, México. Rev. Mex. Fitopatol. 20: 45–52.

    Google Scholar 

  • Goldbach, R., Bucher, E., and Prins, M. 2003. Resistance mechanisms to plant viruses. Virus Res. 92: 207–212.

    Google Scholar 

  • Golden, T., Schauer, S., Lang, J., Pien, S., Mushegian, A., Grossniklaus, U., Meinke, D. and Ray, A. 2002. Short integuments1/suspensor1/carpel factory, a Dicer homolog, is a maternal effect gene required from embryo development in Arabidopsis. Plant Physiol. 130: 808–822.

    Google Scholar 

  • Gonsalves, C. 2001. Transgenic virus-resistant papaya: farmer adoption and impact in the Puna area of Hawaii. Master in Liberal Arts. Graduate School-Empire State College, State University of New York. 170.

    Google Scholar 

  • Gonsalves, C., Cai, W., Tennant, P., and Gonsalves, D. 1998. Effective development of Papaya ringspot virus resistant papaya with untranslatable coat protein gene using a modified microprojectile transformation method. Acta Hortic. 461: 311–314.

    Google Scholar 

  • Gonsalves, D. 1998. Control of Papaya ringspot virus in papaya: a case study. Ann. Rev. PhytoPathol. 36: 415–437.

    Google Scholar 

  • Gonsalves, D., and Fermin, G. 2003. Transgenic papaya to control Papaya ringspot virus in Hawaii and technology transfer to other countries (In: Handbook of Biotechnology). John Wiley & Sons (accepted for publication).

    Google Scholar 

  • Gonsalves, D., and Trujillo, E. 1986. Tomato spotted wilt virus in papaya and detection of the virus by ELISA. Plant Dis. 70: 501–506.

    Google Scholar 

  • Hamilton, A., and Baulcombe, D. 1999. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286: 950–952.

    Google Scholar 

  • Hammond, J., Lecoq, H., and Raccah, B. 1999. Epidemiological risks from virus mixed infections and transgenic plants expressing viral genes. Adv. Virus Res. 54: 189–314.

    Google Scholar 

  • Hannon, G. 2002. RNA interference. Nature 418: 244–251.

    Google Scholar 

  • Jacobsen, S., Running, M., and Meyerowitz, E. 1999. Disruption of an RNA helicase/RNAseIII gene in Arabidopsis causes unregulated cell division in floral meristems. Development 126: 5231–5243.

    Google Scholar 

  • Jan, F.-J. 1998. Roles of non-target DNA and viral gene length in influencing multi-virus resistance through homology-dependent gene silencing. Faculty of the Graduate School, Cornell University. 286.

    Google Scholar 

  • Jan, F.-J., Fagoaga, C., Pang, S.-Z., and Gonsalves, D. 2000a. A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. J. Gen. Virol. 81: 235–242.

    Google Scholar 

  • Jan, F.-J., Fagoaga, C., Pang, S.-Z., and Gonsalves, D. 2000b. A single chimeric transgene derived from two distinct viruses confers multi-virus resistance in transgenic plants through homology-dependent gene silencing. J. Gen. Virol. 81: 2103–2109.

    Google Scholar 

  • Jensen, D. 1949. Papaya virus diseases with special reference to papaya ringspot. Phytopathology 39: 191–211.

    Google Scholar 

  • Kasschau, K., and Carrington, J. 1998. A counterdefensive strategy of plant viruses: suppression of post-transcriptional gene silencing. Cell 95: 461–470.

    Google Scholar 

  • Kawano, S., and Yanaha, T. 1992. The occurrence of Papaya leaf distortion mosaic virus in Okinawa. FFTC Tech. Bull. 132: 12–23.

    Google Scholar 

  • Kiritani, K., and Su, H. J. 1999. Papaya ringspot, banana bunchy top, and citrus greening in the Asia and Pacific region: Occurrence and control strategy. Jarq — Jpn. Agric. Res. Q. 33: 23–30.

    Google Scholar 

  • Kitajima, E., Ritzinger, C., and Rodrigues, M. 1991. Rhabdovirus-like particles in some compositae squash and papaya. Fitopatol. Bras. 16: 141–144.

    Google Scholar 

  • Kitajima, E., Rodrigues, C., Silveira, J., Alves, F., Ventura, J., Aragão, F., and Oliveira, C. 1993. Association of isometric virus-like particles, restricted to laticifers, with “meleira” (“sticky disease”) of papaya (Carica papaya). Fitopatol. Bras. 8: 118–122.

    Google Scholar 

  • Kooter, J., Matzke, M., and Meyer, P. 1999. Listening to the silent genes: transgene silencing, gene regulation and pathogen control. Trends Plant Sci. 4: 340–347.

    Google Scholar 

  • Lastra, R., and Quintero, E. 1981. Papaya apical necrosis, a new disease associated with a rhabdovirus. Plant Dis. 65: 439–440.

    Google Scholar 

  • Lecoq, H., Dafalla, G., Desbiez, C., Wipf-Scheibel, C., Delécolle, B., Lanina, T., Ullah, Z. and Grumet, R. 2001. Biological and molecular characterization of Moroccan watermelon mosaic virus and a potyvirus isolate from Eastern Sudan. Plant Dis. 85: 547–552.

    Google Scholar 

  • Lima, R., Lima, J., Souza Jr., M., Pio-Ribeiro, G., and Andrade, G. 2001. Etiologia e estratégias de controle de viroses do mamoeiro no Brasil. Fitopatol. Bras. 26: 689–702.

    Google Scholar 

  • Lindbo, J., Silva-Rosales, L., Proebsting, W., and Dougherty, W. 1993. Induction of a highly specific antiviral state in transgenic plants— implications for regulation of gene-expression and virus resistance. Plant Cell 5: 1749–1759.

    Google Scholar 

  • Lines, R., Persley, D., Dale, J., Drew, R., and Bateson, M. 2002. Genetically engineered immunity to Papaya ringspot virus in Australian papaya cultivars. Mol. Breeding 10: 119–129.

    Google Scholar 

  • Ling, K., Namba, S., Gonsalves, C., Slightom, J., and Gonsalves, D. 1991. Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the Papaya ringspot virus coat protein gene. Bio/Technol. 9: 752–758.

    Google Scholar 

  • Lius, S., Manshardt, R., Fitch, M., Slightom, J., Sanford, J., and Gonsalves, D. 1997. Pathogen-derived resistance provides papaya with effective protection against Papaya ringspot virus. Mol. Breeding 3: 161–168.

    Google Scholar 

  • Loreto, T., Vital, A., and Rezende, J. 1983. Ocorrência de um amarelo letal do mamoeiro solo no estado de Pernambuco. O biologico 49: 275–279.

    Google Scholar 

  • Maciel-Zambolim, E., Kunieda-Alonso, S., Matsuoka, K., de Carvalho, M., and Zerbini, F. 2003. Purification and some properties of Papaya meleira virus, a novel virus infecting papayas in Brazil. Plant Pathol. 52: 389–394.

    Google Scholar 

  • Manshardt, R. 1992. Papaya. Chapter 21 Chapter 21 in: Biotechnology of Perennial Fruit Crops. F. Hammerschlag and R. Litz. CAB International Press, Cambridge

    Google Scholar 

  • Manshardt, R. 1998. ‘UH Rainbow’ papaya. University of Hawaii, College of Tropical Agriculture and Human Resources Germplasm, Honolulu. G-1: 2pp

    Google Scholar 

  • Marys, E., Carballo, O., and Izaguirre-Mayoral, M. L. 1995. Properties of a previously undescribed supercoiled filamentous virus infecting papaya in Venezuela. Arch. Virol. 140: 891–898.

    Google Scholar 

  • Marys, E. E., Carballo, O., and Izaguirre-Mayoral, M. L. 2000. Occurrence and relative incidence of viruses infecting papaya in Venezuela. Ann. appl. Biol. 136: 121–124.

    Google Scholar 

  • Matzke, M., and Matzke, A. 1998. Epigenetic silencing of plant transgenes as a consequence of diverse cellular defence responses. Cell. Mol. Life Sci. 54: 94–103.

    Google Scholar 

  • Ming, Y., Dong, W., Hong, C., Yu, L., and Min, Z. 2003. Analyses of virus resistance and transgenes for transgenic papaya. Yichuan 25: 181–184.

    Google Scholar 

  • Mueller, E., Gilbert, J., Davenport, G., Brigneti, G., and Baulcombe, D. 1995. Homologydependent resistance: transgenic virus resistance in plants related to homology-dependent gene silencing. Plant J. 7: 1001–1013.

    Google Scholar 

  • Nadeem, A., Mehmood, T., and Tahir, M. 1997. First report of papaya leaf curl disease in Pakistan. Plant Dis. 81: 1333.

    Google Scholar 

  • Nelson, R., Powell-Abel, P., and Beachy, R. 1987. Lesion and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein gene of Tobacco mosaic virus. Virology 158: 126–132.

    Google Scholar 

  • Noa-Carrazana, J., and Silva-Rosales, L. 2001. First report of a Mexican isolate of Papaya mosaic virus in papaya (Carica papaya) and pumpkin (Cucurbita pepo). Plant Dis. 85: 558.

    Google Scholar 

  • Osbourn, J., Watts, J., and Beachy, R. 1989. Evidence that nucleocapsid disassembly and a later step in virus replication are inhibited in transgenic tobacco expressing TMV coat protein. Virology 172: 370–373.

    Google Scholar 

  • Pang, S.-Z., Jan, F.-J., and Gonsalves, D. 1997. Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tosposvirus resistance in transgenic plants. Proc. Natl. Acad. Sciences, USA 94: 8261–8266.

    Google Scholar 

  • Prins, M., de Haan, P., Luyten, R., van Veller, M., van Grinsven, M., and Goldbach, R. 1995. Broad resistance to tospoviruses in transgenic tobacco plants expressing three tospoviral nucleoprotein gene sequences. Mol. Plant Microbe In. 8: 85–91.

    Google Scholar 

  • Purcifull, D., Edwardson, J., Hiebert, E., and Gonsalves, D. 1984. Papaya ringspot virus. CMI/AAB descriptions of plant viruses 292 (No 84 Revis., July 1984): 8.

    Google Scholar 

  • Purcifull, D., and Hiebert, E. 1971. Papaya mosaic virus. CMI/AAB descriptions of plant viruses 56p.

    Google Scholar 

  • Quemada, H., Gonsalves, D., and Slightom, J. 1991. Expression of coat protein gene from Cucumber mosaic virus strain-C coat in tobacco: protection against infection by CMV strains transmitted mechanically or by aphids. Phytopathology 81: 794–802.

    Google Scholar 

  • Quemada, H., Hostis, B., Gonsalves, D., Reardon, I., Heinrikson, R., Hiebert, E., Sieu, L. and Slightom, J. 1990. The nucleotide sequence of the 3′ terminal regions of papaya ringspot virus strains W and P. J. Gen. Virol. 71: 203–210.

    Google Scholar 

  • Rajapakse, R. H. S. 1981. Host Susceptibility of the Papaya Mosaic-Virus in Sri-Lanka. Beitr. Trop. Landwirt. 19: 429–432.

    Google Scholar 

  • Rezende, J., and Costa, A. 1987. Four viruses infecting papaya plants experimentally. Fitopatol. Bras. 12: 63–65.

    Google Scholar 

  • Robinson, D. 1996. Environmental risks assessment of releases of transgenic plants containing virus-derived inserts. Transgenic Res. 5: 359–362.

    Google Scholar 

  • Ruibal-Mendieta, N., Nagy, A.-M., and Lints, F. 1997. The potential allergenicity of novel foods. J. Sci. Food Agr. 75: 405–411.

    Google Scholar 

  • Sanford, J., and Johnston, S. 1985. The concept of pathogen derived resistance: deriving resistance genes from the parasite’s own genome. J. Theor. Biol. 113: 395–405.

    Google Scholar 

  • Saxena, S., Hallan, V., Singh, B., and Sane, P. 1998. Nucleotide sequence and intergeminiviral homologies of the DNA-A of papaya leaf curl geminivirus from India. Biochem. Mol. Biol. Int. 45

    Google Scholar 

  • Shukla, D. D., Ward, C.W., and Brunt, A.A 1994. The Potyviridae. CAB International, Wallingford.

    Google Scholar 

  • Sit, T. L., Abouhaidar, M. G., and Holy, S. 1989. Nucleotide-Sequence of Papaya mosaic virus RNA. J. Gen. Virol. 70: 2325–2331.

    Google Scholar 

  • Souza Jr., M. 1999. Analysis of the resistance in genetically engineered papaya against Papaya ringspot potyvirus, partial characterization of the PRSV Brazil Bahia isolate, and development of transgenic papaya for Brazil. Doctor al. Thesis. Department of Plant Pathology, Cornell University. 277.

    Google Scholar 

  • Strating, A. 1996. Availability of determination of nonregulated status for papaya lines genetically engineered for virus resistance. Fed. Regist. 61: 48663.

    Google Scholar 

  • Taya, R., and Singh, J. 1995. Survey of Papaya mosaic virus disease in Haryana. Agr. Sci. Dig. 15: 191–192.

    Google Scholar 

  • Tennant, P. 1996. Evaluation of the resistance of coat protein transgenic papaya against Papaya ringspot virus isolates and development of transgenic papaya for Jamaica. Faculty of Graduate Studies, Cornell University. 317.

    Google Scholar 

  • Tennant, P., Ahmad, M., and Gonsalves, D. 2002. Transformation of Carica papaya L. with virus coat protein genes for studies on resistance to Papaya ringspot virus from Jamaica. Trop. Agr. 79: 105–113.

    Google Scholar 

  • Tennant, P., Fermin, G., Fitch, M., Manshardt, R., Slightom, J., and Gonsalves, D. 2001. Papaya ringspot virus resistance of transgenic ‘Rainbow’ and’ sunUp’ is affected by gene dosage, plant development, and coat protein homology. Eur. J. Plant Pathol. 107: 645–653.

    Google Scholar 

  • Tennant, P., Gonsalves, C., Ling, K., Fitch, M., Manshardt, R., Slightom, J., and Gonsalves, D. 1994. Differential protection against Papaya ringspot virus isolates in coat protein transgenic papaya and classically cross-protected papaya. Phytopathology 84: 1359–1366.

    Google Scholar 

  • Thomas, J., and Dodman, R. 1993. The first record of Papaya ringspot virus-type P in Australia. Aust. Plant Pathol. 22: 2–7.

    Google Scholar 

  • Tricoli, D., Carney, K., Russell, P., McMaster, J., Groff, D., Haden, K., Himmel, P., Hubbard, J., Boeshore, M., and Quemada, H. 1995. Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to Cucumber mosaic virus, Watermelon mosaic virus 2, and Zucchini yellow virus. Bio/Technol. 13: 1458–1465.

    Google Scholar 

  • Valand, G., and Muniyapra, V. 1992. Epidemiology of Tobacco leaf curl virus in India. Ann. appl. Biol. 120: 257–267.

    Google Scholar 

  • van den Boogaart, T., Lomonossoff, G., and Davies, J. 1998. Can we explain RNA-mediated virus resistance by homology-dependent gene silencing? Mol. Plant Microbe In. 11: 717–723.

    Google Scholar 

  • Wasseneger, M., and Pelissier, T. 1998. A model for RNA-mediated gene silencing in higher plants. Plant Mol. Biol. 37: 349–362.

    Google Scholar 

  • Waterhouse, P., Wang, M.-B., and Finnegan, E. 2001. Role of short RNAs in gene silencing. Trends Plant Sci. 6: 297–301.

    Google Scholar 

  • Wilson, T., and Watkins, P. 1986. Influence of exogenous viral coat protein on the cotranslational disassembly of Tobacco mosaic virus. Virology 149: 132–135.

    Google Scholar 

  • Wisnieswski, L., Powell, P., Nelson, R., and Beachy, R. 1990. Local and systemic spread of Tobacco mosaic virus in transgenic tobacco. Plant Cell 2: 559–567.

    Google Scholar 

  • Yeh, S., and Gonsalves, D. 1985. Translation of Papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindricalinclusion protein, and amorphous-inclusion protein. Virology 143: 260–271.

    Google Scholar 

  • Yeh, S.-D., and Gonsalves, C. 1984. Evaluation of induced mutants of Papaya ringspot virus for control by cross protection. Phytopathology 74: 1086–1091.

    Google Scholar 

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Fermin, G., Gonsalves, D. (2003). Papaya. In: Loebenstein, G., Thottappilly, G. (eds) Virus and Virus-like Diseases of Major Crops in Developing Countries. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0791-7_20

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  • DOI: https://doi.org/10.1007/978-94-007-0791-7_20

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