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Mouse as a Model Organism pp 11–44Cite as

The German Mouse Clinic – Running an Open Access Platform

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Abstract

Next challenges in functional annotation of mammalian genomes are yet of a much larger scope than previous genomics initiatives. Mouse mutant resources must be phenotyped systematically (one after the other) and systemically (assessing all organ systems). In addition, for the next generation of mouse models the “envirotypes”, that humans are exposed to, need to be modeled. We established the German Mouse Clinic (GMC) as a mouse phenotyping platform for systemic, standardized phenotypic analysis and interpretation and open access for the scientific community on a collaborative basis. Next to phenotyping mouse models from national and international programs we provide the logistics to analyse mutant mouse lines from different origins (e.g., Knock-outs, Knock-ins, Gene-traps, RNAi Knock-downs, ENU mutagenesis, QTL studies) from collaboration partners throughout the world. Here we present an overview of how we organize a high-throughput screening of mouse lines from different laboratories and describe specific methods of selected screens in more detail.

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Notes

  1. 1.

    Partners are the US-based Kockout Mouse Project (KOMP, http://www.knockoutmouse.org/) and Texas A&M Institute of Genomic Medicine (TIGM, http://www.tigm.org/), the European Conditional Mouse Mutagenesis (EUCOMM, http://www.eucomm.org/) and the North American Conditional Mouse Mutagenesis Project (NorCOMM, http://www.norcomm.org/) in Canada.

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Acknowledgments

We would like to thank Reinhard Seeliger, Miriam Backs, Ingrid Bayer, Nicole Boche, Sabrina Bothur, Sandra Geissler, Michaela Grandl, Tamara Halex, Elfi Holupirek, Sabine Holthaus, Constanze König, Maria Kugler, Albert Langer, Katrin Laube, Astrid Markert, Jacqueline Müller, Elenore Samson, Florian Schleicher, Daniela Schmidt, Waldemar Schneider, Ann-Elisabeth Schwarz, Bettina Sperling, Waldtraud Stettinger, Lucie Thurmann, Susanne Wittich, and Claudia Zeller as well as the GMC animal caretaker team for expert technical help. This work has been funded by the BMBF (DZD e.V. 01GI0923 and NGFNplus grant, 01GS08156, 01GS0850, 01GS0851, 01GS0852, 01GS0868, 01GS0869, 01GS0853, and 01GS0854) and by an EU grant (EUMODIC, LSHG-2006-037188, German Mouse Clinic).

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  1. Institute of Experimental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich/Neuherberg, Germany

    Valérie Gailus-Durner, Beatrix Naton, Luciana Afonso, Christian Cohrs, Wolfgang Hans, Marion Horsch, Melanie Kahle, Christoph Lengger, Tonia Ludwig, Holger Maier, Kateryna Micklich, Susanne Neschen, Evelyn Schiller, Anja Schrewe, Markus Scheerer, Felix Schöfer, Ralph Steinkamp, Claudia Stöger, Frank Thiele, Irina Treise, Monja Willershäuser, Ramona Zeh, Johannes Beckers, Helmut Fuchs & Martin Hrabě de Angelis

  2. Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany

    Thure Adler & Dirk H. Busch

  3. Division of Environmental Dermatology and Allergy, Technische Universität München/Helmholtz Zentrum München, Munich, Germany

    Juan Antonio Aguilar-Pimentel & Markus Ollert

  4. Department of Dermatology and Allergy Biederstein, Technische Universität München, Munich, Germany

    Juan Antonio Aguilar-Pimentel & Markus Ollert

  5. Department of Neurology, Friedrich-Baur-Institut, Ludwig-Maximilians-Universität München, Munich, Germany

    Lore Becker & Thomas Klopstock

  6. Institute of Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich/Neuherberg, Germany

    Julia Calzada-Wack, Patricia da Silva-Buttkus, Frauke Neff, Monica Tost, Irene Esposito & Heinz Höfler

  7. Institute of Experimental Genetics, Genome Analysis Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich/Neuherberg, Germany

    Gabriele Möller, Cornelia Prehn & Jerzy Adamski

  8. Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany

    Birgit Rathkolb & Eckhard Wolf

  9. Molecular Nutritional Medicine, ZIEL Research Center for Nutrition and Food Sciences, Technische Universität München, Freising-Weihenstephan, Germany

    Jan Rozman & Martin Klingenspor

  10. Division of Cardiology, Department of Medicine III, University of Heidelberg, Heidelberg, Germany

    Raffi Bekeredjian & Hugo Katus

  11. Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany

    Johannes Beckers & Martin Hrabě de Angelis

  12. Institute of Pathology, Technische Universität München, Munich, Germany

    Irene Esposito

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  1. Valérie Gailus-Durner
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Corresponding author

Correspondence to Valérie Gailus-Durner .

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  1. , Institute of Biomedicine, BRIC, University of Copenhagen, Ole Maaloes Vej 5, Copenhagen, DK-2200, Denmark

    Cord Brakebusch

  2. Oulu Center for Cell-Matrix Research, Oulu, FIN90014, Finland

    Taina Pihlajaniemi

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Gailus-Durner, V. et al. (2011). The German Mouse Clinic – Running an Open Access Platform. In: Brakebusch, C., Pihlajaniemi, T. (eds) Mouse as a Model Organism. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0750-4_2

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