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Malignant Gliomas: Role of E2F1 Transcription Factor

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Tumors of the Central Nervous System, Volume 1

Part of the book series: Tumors of the Central Nervous System ((TCNS,volume 1))

Abstract

E2F1 is a transcription factor regulated by the Rb pathway. Its role in the G1/S transition and S-phase entry in the cell cycle has been extensively reported. E2F1 regulates the biosynthetic activity of nucleotides, the activation of replication, the progression through M-phase of the cell cycle, and the regulation of genes related with chromosomal stability. In addition to the E2F1 crucial involvement in the cell cycle progression, E2F1 participates in both p53-dependent and independent apoptotic pathways. Our group has previously published the presence of high expression of E2F1 in malignant gliomas and the correlation of levels of expression with prognostic factor. Moreover, we reported the direct implication of E2F1 in tumorigenicity using transgenic animal models. Recent results from The Cancer Genome Atlas Research Network confirms the presence in human glioblastomas of the genetic alterations in the Rb tumor suppressor pathway, supporting previous reports describing high E2F1 activity in gliomas. In this chapter, we describe methodology to examine the presence of high expression and activity of E2F1, and a practical approach to examine the targeting of this major transcription factor.

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Acknowledgement

We thank Ann Sutton (Department of Scientific Publication, The University of Texas M. D. Anderson Cancer Center, Houston, TX) for editorial assistance. This work was supported by an Institutional Research Grant from M. D. Anderson Cancer Center.

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Authors and Affiliations

  1. Department of Neuro-Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA

    Marta M. Alonso, Juan Fueyo & Candelaria Gomez-Manzano

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  1. Marta M. Alonso
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  2. Juan Fueyo
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  3. Candelaria Gomez-Manzano
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Correspondence to Candelaria Gomez-Manzano .

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Editors and Affiliations

  1. Dept. Biological Sciences, Kean University, 1000 Moris Avenue, Union, 07083, New Jersey, USA

    M. A. Hayat

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Alonso, M.M., Fueyo, J., Gomez-Manzano, C. (2011). Malignant Gliomas: Role of E2F1 Transcription Factor. In: Hayat, M. (eds) Tumors of the Central Nervous System, Volume 1. Tumors of the Central Nervous System, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0344-5_10

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  • DOI: https://doi.org/10.1007/978-94-007-0344-5_10

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  • Publisher Name: Springer, Dordrecht

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  • Online ISBN: 978-94-007-0344-5

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