Skip to main content
SpringerLink
Log in

Nanostructured Silicon and its Application as the Transducer in Immune Biosensors

  • Conference paper
  • First Online:
Biodefence

Abstract

New biosensors based on detection of the formation of specific antibody-antigen (Ab-Ag) immune complexes using the structured nanoporous silicon (sNPS) are proposed. We used boron doped single-crystal silicon with square wafers of 0.3 mm thickness and resistance of 1 Ohm*cm. sNPS layers with thickness of 3–60 nm were prepared. Electrical contacts were formed by magnetron sputtering of Al. The formation of the immune complex (specific antibody-T2 mycotoxin) was detected by the 2–5-fold increase of current depending on the antigen concentration in the sample. At the same time the sNPS photoluminescence sharply decreased. These biosensors may be applied for measuring the concentration of different substances which are capable of forming a specific complex. The proposed method may provide a simple and cost effective procedure for express control of toxic substances in field conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Luchenko AI, Melnichenko MM, Svezhentsova KV, Shmyryeva OM (2006) Complex studies of properties of nanostructured silicon. Proc of SPIE 6327:1–11

    Google Scholar 

  2. Starodub NF, Starodub VM (2002) Porous silicon: some theoretical aspects and practical application as transducer for immune sensor. In: Extended abstracts of the 3rd international conference porous semiconductors science and technology, Puerto de la Cruz, Tenerife, Spain, pp 155–157, 10–15 Mar 2002

    Google Scholar 

  3. Starodub NF, Starodub VM (2004) Biosensors based on the photoluminescence of porous silicon: overall characteristics and application for the medical diagnostics. Sensors Electronics and Microsystem Technol 2:63–83

    Google Scholar 

  4. Uhlir JrA (1956) Electrolytic shaping of Ge and Si. Bell Syst Tech J N35:333–347

    Google Scholar 

  5. Fauchet PM (1998) Porous silicon: photoluminescence and electroluminescent devices. In: Semiconductors and semimetals, vol 49. Academic, San Diego, pp 206–252

    Google Scholar 

  6. Bsiesy A, Vial JC, Gaspard F et al (1991) Photoluminescence of high porosity and of ­electrochemicaly oxidized p-Si layers. Surf Sci N254:195–200

    Article  Google Scholar 

  7. Canham LT (1997) Porous semiconductors: a tutorial review. Proc Mat Res Soc Symp 452:29–42

    Article  CAS  Google Scholar 

  8. Canham LT (1990) Silicon quantum wire array fabrication by electrochemical and chemical dissolution of wafers. Appl Phys Lett N57:1046–1048

    Article  Google Scholar 

  9. Gardelis S, Rimmer JS, Dawson P et al (1991) Evidence for quantum confinemenr in the photoluminesce of p-Si and SiGe. Appl Phys Lett 59:2128–2120

    Article  Google Scholar 

  10. Koshida N, Koyama H (1991) Efficient visible photoluminescence from p-Si. Jpn J Appl Phys N30:L1221–L1223

    Article  Google Scholar 

  11. Lackwood DJ (1998) Light emission in silicon. In: Semiconductors and semimetals, vol 49. Academic, San Diego, pp 1–35

    Google Scholar 

  12. Svechnikov SV, Sachenko AV, Sukach GA et al (1994) Light emitted layers of the porous silicon: preparation, abilities and application (review). Optoelectronics and Semiconductor Technique N27:3–28

    Google Scholar 

  13. Kim SJ, Jeon BH, Choi KS (1999) Improvement of the sensitivity by UV light in alcohol sensors using porous silicon layer. In: CAS’99 Proceedings of the international semiconductor conference, Sinaia, Romania, 2:475–478

    Google Scholar 

  14. Rittersma ZM, Benecke W (1999) A novel capacitive porous silicon humidity sensor with integrated thermo- and refresh resistors gas sensors. In: Proceedings of 13th European conference on solid-state transducers “Eurosensors XIII”, The Hague, Netherlands, pp 371–374, 12–15 Sept 1999

    Google Scholar 

  15. Sorli B, Garcia M, Benhida A et al (1999) Porous silicon layer used as a humidity sensor. In: Proceedings of the european matter conference E-MRS spring meeting. Symposium I: microcrystalline and nanocrystalline semiconductors, I–8

    Google Scholar 

  16. Thust M, Schoening MJ, Frohnhoff S et al (1996) Porous silicon as a substrate material for potentiometric biosensors. Meas Sci Technol N7:26–29

    Article  Google Scholar 

  17. Bogue RW (1997) Novel porous silicon biosensor. BIOSENS BIOELECTRON 12:xxvii–xxix

    Article  Google Scholar 

  18. Schoening MJ, Ronkel F, Crott M et al (1997) Miniaturization of potentiometric sensors using porous silicon microtechnology. Electrochim Acta 42:3185–3193

    Article  Google Scholar 

  19. Starodub NF, Fedorenko LL, Starodub VM et al (1996) Extinguishing of visible photoluminescence of porous silicon – stimulated by antigen-antibody immunocomplecx formation. In: Optical organic and semiconductor inorganic materials, Riga, Latvia, pp 73–76, 26–29 Aug 1996

    Google Scholar 

  20. Starodub VM, Fedorenko LL, Starodub NF (1998) Control of a myoglobin level in solution by the bioaffine sensor based on the photoluminescence of porous silicon. In: Proceedings of the european conference on solid-state transducers and 9th UK conference on sensors and their applications, Southampton, UK, 2:817–820, 13–16 Sept 1998

    Google Scholar 

  21. Starodub VM, Fedorenko LL, Sisetskiy AP, Starodub NF (1999) Control of myoglobin level in an immune sensor based on the photoluminescence of porous silicon. Sensors and Actuator B58:409–414

    Article  CAS  Google Scholar 

  22. Starodub VM, Starodub NF (1999) Optical immune sensors for the monitoring protein substances in the air. In: Eurosensor XII and 13th european conference on solid-state transducers. The Hague, Netherlands, pp 181–184, 12–15 Sept 1999

    Google Scholar 

  23. Starodub VM, Fedorenko LL, Starodub NF (2000) Optical immune sensor for the monitoring protein substances in the air. Sensors and Actuator B68:40–47

    Article  CAS  Google Scholar 

  24. Burmeister HR (1971) T-2 toxin production by Fusarium tricinctum on solid substrate. Appl Microbiol 21:739–742

    PubMed  CAS  Google Scholar 

  25. Cole RJ, Cox RH (1981) The trichothecenes. In: Cole RJ, Cox RH (eds) Handbook of toxic fungal metabolites. Academic, New York, NY, pp 152–263

    Google Scholar 

  26. Miller JD, Taylor A, Greenhalgh R (1983) Production of deoxynivalenol and related compounds in liquid culture by Fusarium graminearum. Can J Microbiol 29:1171–1178

    Article  CAS  Google Scholar 

  27. Madsen JM (2001) Toxins as weapons of mass destruction. A comparison and contrast with biological-warfare and chemical-warfare agents. Clin Lab Med 21:593–605

    PubMed  CAS  Google Scholar 

  28. Starodub NF (2009) Biosensors in a system of instrumental tools to prevent effects of bioterrorism and automotive control of water process purification. In: Jones JAA et al (eds) Proceedings of NATO science for peace and security, series C: environmental security, Threats to global water security, Springer Sci + Business Media BV, 59–71

    Google Scholar 

  29. Starodub NF (2009) Biosensors in the system of express control of chemicals regularly used as terrorist means to prevent non diserable consequences. In: The role of ecological chemistry in pollution research and sustainable development. Springer Sci + Business Media BV, 275–384

    Google Scholar 

  30. Bunner DL, Upshall DG, Bhatti AR (1985) Toxicology data on T-2 toxin. In: Report of focus officers meeting on mycotoxin toxicity, Defense research establishment at Suffield Alta, Canada, 23–24 Sept 1985

    Google Scholar 

  31. Artjuch VP, Gojster OS, Khmelnitsky GA, Starodub NF (2003) Trichtezene mycotoxins: determination in environmental objects. Biopolym Cell 19:216–223

    Google Scholar 

  32. Starodub NF, Pylipenko LN, Egorova AV et al (2008) Analysis of mycotoxins: preparations of samples. Biotechnology 1:106–115

    Google Scholar 

  33. Starodub NF, Pylipenko LN, Egorova AV, Pylipenko IV (2008) Mycotoxin patulin: producers, biological effects, indication in foods. Mod Probl Toxicol 3:50–57

    Google Scholar 

  34. Starodub NF, Kanjuk MI, Ivashkevich SP et al (2009) Determination of patulin toxicity and its control in environment by optical biosensor system. In: Proceedings of OPTO 2009&IRS2, Nurenberg, Germany, pp 151–156, 26–28 May 2009

    Google Scholar 

  35. Starodub NF, Dibrova TI, Shirshov YuM, Kostiukevich KV (1997) Development of sensor based on the surface plasmon resonance for control of biospecific interaction. In: Proceedings of eurosensors 12 and 11th european conference on solid-state transducers, Warsaw, Poland, 3:1429–1432, 21–24 Sept

    Google Scholar 

  36. Starodub NF, Dibrova TL, Shirshov YuM, Kostjukevich KV (1999) Development of myoglobin sensor based on the surface plasmon resonance. Ukr Biochem J 71:33–37

    CAS  Google Scholar 

  37. Starodub VM, Starodub NF (2001) Electrochemical and optical biosensors: origin of development, achievements and perspectives of practical application. In: Bozoglu F et al (eds) NATO series novel processes and control technologies in the food industry, Amsterdam, 63–94

    Google Scholar 

  38. Nabok AV, Tsargorodskaya A, Hassan AK, Starodub NF (2005) Total internal reflection ellipsometry and SPR detection of low molecular weight environmental toxins. Appl Surf Sci 246:381–386

    Article  CAS  Google Scholar 

  39. Starodub NF, Nabok AV, Tsargorodskaya A et al (2005) Optical biosensors for the registration of some pesticides, mycotoxins and endocrine disrupting substances in environmental objects. 84 ICB seminar on biochemical sensing – Utilization of micro- and nanotechnologies, Warsaw, p 30

    Google Scholar 

  40. Starodub NF, Pylipeko LN, Pylipenko IV, Egorova AV (2008) Mycotoxins and other low weight toxins as instrument of bioterrorists: express instrumental control and some ways to decontaminate polluted environmental objects. Timisoara Medical J 58:9–18

    Google Scholar 

  41. Starodub MF, Romanov VO, Kochan RV et al (2006) Implementation of SPR-biosensors for express-diagnostics of acute viral infection and mycotoxicosis. Bull Khmelnitski Nat Univ N6:223–226

    Google Scholar 

Download references

Acknowledgments

This work was supported by the State Fund of Fundamental Research of Ukraine, project N F28.7/020 as well as by the Collaborative NATO grant (CBR.NUKR.CLG-983381) and grant of the National University of Life and Environmental Science of Ukraine.

Author information

Authors and Affiliations

  1. National University of Life and Environmental Sciences, 15 Herojev Oboroni Str., Kyiv, 03041, Ukraine

    N. F. Starodub & L. M. Shulyak

  2. National Technical University of Ukraine “KPI”, 37 Prospect Peremogy, Kyiv, 03056, Ukraine

    O. M. Shmyryeva

  3. National Academy of Food Technology, Odessa, Ukraine

    I. V. Pylipenko & L. N. Pylipenko

  4. Taras Shevchenko Kiev National University, 2 Prospect Glushkova, Kyiv, 03127, Ukraine

    M. M. Mel’nichenko

Authors
  1. N. F. Starodub
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. M. Shulyak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. M. Shmyryeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. V. Pylipenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. N. Pylipenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. M. Mel’nichenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. F. Starodub .

Editor information

Editors and Affiliations

  1. School of Pharmacy &, Biomolecular Sciences, University of Brighton, Lewes Road, Brighton, East Sussex, BN2 4GJ, United Kingdom

    Sergey Mikhalovsky

  2. Center for Emergency Medicine, Ministry of Public Health, Republican Specialised Scientific, Tashkent, 100115, Uzbekistan

    Abdukhakim Khajibaev

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media B.V.

About this paper

Cite this paper

Starodub, N.F., Shulyak, L.M., Shmyryeva, O.M., Pylipenko, I.V., Pylipenko, L.N., Mel’nichenko, M.M. (2011). Nanostructured Silicon and its Application as the Transducer in Immune Biosensors. In: Mikhalovsky, S., Khajibaev, A. (eds) Biodefence. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0217-2_9

Download citation

Publish with us

Policies and ethics

Search

Navigation