Abstract
Identification of brassinosteroids (BS) in plants is rather time consuming and laborious process involving many operations. The highest measured level of BS in plant tissues is about 10%-5, and the expenditure rates in agricultural use vary in a range of 5–50 mg/ha. Such concentrations create a serious problem for measuring BS in plant sources that is of crucial importance for investigation of their biosynthesis and metabolism and for their efficient practical application. The known chromatographic methods of BS analysis suffer from the relatively long analysis time, laborious and expensive sample cleanup prior to analysis and the need of rather sophisticated equipment. As a result, they are not suitable for routine analysis of samples. A good alternative to chromatographic methods is the immunochemical approach because of simplicity of equipment and measuring procedure. There is no doubt that namely this type of assay is the most realistic for wide-scale practical applications. The present chapter summarizes the latest developments in this field with an emphasis on contributions made in authors’ laboratory.
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Khripach, V.A., Zhabinskii, V.N., Litvinovskaya, R.P. (2011). Immunoassays of brassinosteroids. In: Hayat, S., Ahmad, A. (eds) Brassinosteroids: A Class of Plant Hormone. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0189-2_14
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DOI: https://doi.org/10.1007/978-94-007-0189-2_14
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