Microscale Flow Dynamics of Red Blood Cells in Microchannels: An Experimental and Numerical Analysis
The blood flow dynamics in microcirculation depends strongly on the microvascular networks composed with short irregular vessel segments which are linked by numerous bifurcations. This paper presents the application of a confocal micro-PTV system to track RBCs through a rectangular polydimethysiloxane (PDMS) microchannel with a bifurcation. By using a confocal micro-PTV system, we have measured the effect of bifurcation on the flow behaviour of both fluorescent particles diluted in pure water and RBCs in concentrated suspensions. After performing simulations with the commercial finite element software package POLYFLOW ®;, some experimental results were compared with the numerical results and the limitations of these simulations were outlined.
KeywordsBlood flow Microvascular networks Bifurcation Microchannel
This study was supported in part by the following grants: Grant-in-Aid for Science and Technology (BII/UNI/0532/EME/2008, PTDC/SAU-BEB/108728/2008, PTDC/SAU-BEB/105650/2008 and PTDC/EME-MFE/099109/2008) from the Science and Technology Foundation (FCT) and COMPETE, Portugal and Grant-in-Aid for Scientific Research (S) from the Japan Society for the Promotion of Science (JSPS; No.19100008). We also acknowledge the support from the 2007 Global COE Program “Global Nano-Biomedical Engineering Education and Research Network”. The authors would like also to thank Dr. C. Balsa for his valuable assistance and support for the MATLAB numerical calculations and Ms. B. Oliveira, Ms. D. Cidre and Mr. M. Lagoela for their valuable technical assistance in this researchwork.
- 1.Abramoff, M., Magelhaes, P., Ram, S.: Image processing with image J. Biophotonics Int. 11, 36–42(2004)Google Scholar
- 3.Chien, S., Usami, S., Skalak, R.: Blood flow in small tubes In: Renkins, M., Michel, C.C. (eds.) Handbook of Physiology–The Cardiovascular System IV, pp.217–249. American Physiological Society, Bethesda(1984)Google Scholar
- 9.Fernandes, C.S., Dias, R.P., etal.: Laminar flow in chevron-type plate heat exchangers: CFD analysis of tortuosity, shape factor and friction factor. Chem. Eng. Process.: Process Intensif. 46, 825–833(2007)Google Scholar
- 11.Goldsmith, H., Turitto, V.: Rheological aspects of thrombosis and haemostasis: Basic principles and applications. ICTH-Report-Subcommittee on Rheology of the International Committee on Thrombosis and Haemostasis. Thromb. Haemost. 55, 415–435(1986)Google Scholar
- 14.Lima, R., Ishikawa, T., etal.: Blood flow behavior in microchannels: Advances and future trends. In: Single and Two-Phase Flows on Chemical and Biomedical Engineering. Bentham (in press) (2011)Google Scholar
- 22.Oliveira, B., Lagoela, M., etal.: Analyses of the blood flow in a microchannel with a bifurcation. In: Proceedings of 3∘Congresso Nacional de Biomecânica, Bragança, Portugal(2009)Google Scholar
- 23.Omori, T., Ishikawa, T. etal.: Behavior of a red blood cell in a simple shear flow simulated by a boundary element method, In: Proceedings of Bioengineering 08, London, UK(2008)Google Scholar
- 27.Pries, A., Secomb, T., etal.: Resistance to blood flow in microvessels in vivo. Circ. Res. 75, 904–915(1994)Google Scholar
- 29.Succi, S.: The Lattice Boltzmann Equation for Fluid Mechanics and Beyond, Clarendon Press, Oxford(2001)Google Scholar
- 35.Wilkinson, W.L.: Non-Newtonian fluids: Fluid mechanics, mixing and heat transfer, pp.61–63. Pergamon Press, London(1960)Google Scholar