Abstract
Fluorescent ions and molecules are important probes for the investigation of biological systems. To initiate the fluorescence, such ions and molecules are usually excited by absorption of a single photon. It is often advantageous, however, to excite the molecule using two or more photons. In this article we examine the multiphoton absorption process, and discuss of the advantages and disadvantages it offers for the investigation of biological systems. The main point of this article is to review the basic physics of two-photon absorption process. We also define the two-photon absorption cross section and discuss how it is measured. Finally, the advantages of two photon absorption are discussed and some applications to biological systems are presented.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Lakowicz J. R. (2006), Principles of Fluorescence Spectroscopy, 3rd ed., Springer, New York.
Herschel J. F. W. (1845), Phil. Trans. Roy. Soc. (London) 135, 143
Gregory J. (ed.) (1966) Handbook of fluorescent probes and research products, 9th ed., Molecular Probes Inc., Eugene, OR
Jablonski A. (1935), Z. Phys. 94, 38
Stokes G. G. (1852), Phil. Trans. R. Soc. (London) 142, 463
Kasha M. (1950), Disc. Faraday Soc. 9, 14
Berlman I. B. (1971), Handbook of aromatic molecules, 2nd ed., Academic, New York
Stern O. and Volmer M. (1919), Phys. Z. 20, 183
Albani J. R. (2007), Principles and Applications of Fluorescence Spectroscopy, Blackwell Publishing, New York
Stryer L. (1978), Annu. Rev. Biochem. 47, 819
Berberan-Santos M. N. (2001), in New Trends in Fluorescence Spectroscopy: Applications to Chemical and Life Sciences, 18, 733, B. Valeur and J.C. Brochon eds, Springer, New York
Royer C. A. (1966), Biophys. J. 68, 1191
Eyring H., Walter J and Kimball G. F. (1944), Quantum Chemistry, Wiley, New York, p. 351
Morgenau H. (1931), Phys. Rev. 38, 747
Morgenau H. (1939), Rev. Mod. Phys. 11, 1
Carlson B. C. and Rushbrooke G. S. (1950), Proc. Camb. Phil. Soc. 46, 626
Watts R. K. (1975), in Optical Properties of Ions in Solids, B. Di Bartolo ed., Plenum Press, New York and London, p. 307
Perrin F. (1928), Compt. Rend. 178, 1978
Stern O. and Volmer M. (1919), Physik Z. 20, 183
Förster Th. (1949), Z. Naturforsch 4a, 321
Förster Th. (1959), Discussions Faraday Soc. 27, 7
Galanin M. D, (1955), Sov. Phys. JETP 1, 317
Reif F. (1965), Fundamentals of Statistical and Thermal Physics, McGraw Hill, New York, p.483
Yokota M. and Tanimoto O. (1967), J. Phys. Soc. Japan 22, 779
Watts R. K. and Richter H. J. (1972), Phys. Rev. B6, 1584
Karpick J. T. and Di Bartolo B. (1971), J. of Luminescence 4, 309
Lakowicz J. R. (2006), Principles of Fluorescence Spectroscopy, 3rd ed., Springer, New York
Clegg R. M. (1966), in Fluorescence Imaging Spectroscopy, X. F. Wang and B. Herman, eds., p. 179, Wiley, New York
Yokota M. and Tanimoto O. (1967), J. Phys. Soc. Japan 22(3), 779
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media B.V.
About this paper
Cite this paper
Collins, J. (2011). Two-Photon Absorption and Applications to Biological Systems. In: Bartolo, B., Collins, J. (eds) Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9977-8_12
Download citation
DOI: https://doi.org/10.1007/978-90-481-9977-8_12
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-9976-1
Online ISBN: 978-90-481-9977-8
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)