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Biophotonics: Harnessing Light for Biology and Medicine

Nonlinear Optical Imaging and Light Induced Therapy

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Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation

Abstract

Biophotonics is a new multidisciplinary frontier which utilizes light–matter interactions for bioimaging, sensing diagnostics and light activated as well as optically tracked therapies. This article is focused on use of nonlinear optical processes for bio imaging as well as for light induced therapy. Examples illustrating recent achievements in these research fields are provided from our work at the Institute for Lasers, Photonics and Biophotonics. We present our progress in multimodal bioimaging using a combination of nonlinear optical phenomena such as two-photon induced fluorescence, coherent anti-Stokes Raman scattering (CARS), second harmonic generation and sum frequency generation. Synergy of the techniques employing these phenomena in a multimodal imaging approach enables chemically selective imaging and probing of the local macro molecular content in biological specimens. We also provide examples of two-photon induced fluorescence microscopy and two-photon induced therapy using specially designed molecules and nanoparticles.

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Authors and Affiliations

  1. Institute for Lasers, Photonics and Biophotonics, SUNY at Buffalo, Buffalo, NY, 14260, USA

    T. Y. Ohulchanskyy, A. M. Pliss & P. N. Prasad

Authors
  1. T. Y. Ohulchanskyy
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  2. A. M. Pliss
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  3. P. N. Prasad
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Corresponding author

Correspondence to P. N. Prasad .

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Editors and Affiliations

  1. Dept. Physics, Boston College, Commonwealth Ave 140, Chestnut Hill, 02467, Massachusetts, USA

    Baldassare Di Bartolo

  2. , Department of Physics and Astronomy, Wheaton College, Norton, 02766, Massachusetts, USA

    John Collins

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Ohulchanskyy, T.Y., Pliss, A.M., Prasad, P.N. (2011). Biophotonics: Harnessing Light for Biology and Medicine. In: Bartolo, B., Collins, J. (eds) Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9977-8_1

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