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Quantitative Approaches to Nuclear Architecture Analysis and Modelling

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Advances in Nuclear Architecture

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Abstract

The spatial organisation of the genome in the cell nucleus has emerged as a key element to understand gene function. A wealth of molecular and microscopic information has been accumulated, resulting in a variety of – sometimes contradictory – models of nuclear architecture. So far, however, a large part of this structural information and in consequence also the models derived from them are ‘qualitative’. In this overview, a brief introduction will be given into quantitative experimental and modelling approaches to large scale nuclear genome architecture in human cells. As a biomedical application example, the use of a quantitative computer model of the 3D architecture allowed to explore different implications of nuclear structure on chromosomal aberrations. In addition, we shall present two novel examples for quantitative computer modelling: (1) The impact of SC 35 splicing domains on nuclear genome structure; (2) The dynamics of large scale nuclear genome structure in a Brownian motion model. Finally, we shall discuss some perspectives to extend quantitative nuclear structure analysis to the nanoscale.

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Acknowledgements

This work was supported by grants from the Deutsche Forschungsge­meinschaft and the European Union. For discussions and other help we especially thank Manuel Gunkel, Rainer Kaufmann and Thomas Cremer.

Author information

Authors and Affiliations

  1. Applied Optics and Information Processing, University Heidelberg, Im Neuenheimer Feld, D-69120, Heidelberg, Germany

    Daniel Hübschmann & Gregor Kreth

  2. Applied Optics and Information Processing and BioQuant Center, University Heidelberg, Im Neuenheimer Feld, D-69120, Heidelberg, Germany

    Nikolaus Kepper

  3. Applied Optics and Information Processing; BioQuant Center, Institute for Pharmacy and Molecular Biotechnology, University Heidelberg, Im Neuenheimer Feld, D-69120, Heidelberg, Germany

    Christoph Cremer

  4. Institute for Molecular Biophysics, The Jackson Laboratory, 04609, Bar Harbor, ME, USA

    Christoph Cremer

Authors
  1. Daniel Hübschmann
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  2. Nikolaus Kepper
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  3. Christoph Cremer
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  4. Gregor Kreth
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Corresponding authors

Correspondence to Christoph Cremer or Gregor Kreth .

Editor information

Editors and Affiliations

  1. Department of Mathematics, Imperial College, Queen's Gate 180, London, SW7 2AZ, United Kingdom

    Niall M. Adams

  2. Div. Molecular Biosciences, Imperial College London, London, SW7 2AZ, United Kingdom

    Paul S. Freemont

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Hübschmann, D., Kepper, N., Cremer, C., Kreth, G. (2011). Quantitative Approaches to Nuclear Architecture Analysis and Modelling. In: Adams, N., Freemont, P. (eds) Advances in Nuclear Architecture. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9899-3_3

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