Abstract
Regenerative medicine is the application of tissue, sciences, engineering, computations, related biological, and biochemical principles that restore the structure and function of damaged tissues and organs. This new field encompasses many novel approaches to treatment of disease and restoration of biological function. Scientists are one-step closer to create a gene therapy/stem cell combination to combat genetic diseases. This research may lead to not only curing the disease, but also repairing the damage left behind. However, the development of gene therapy vectors with sufficient targeting ability, efficiency, and safety must be achieved before gene therapy can be routinely used in human. Delivery systems based on both viruses and non-viral systems are being explored, characterized, and used for in vitro and in vivo gene delivery. Although advances in gene transfer technology have been made, an ideal system has not yet been constructed. The development of scalable computer systems constitutes one-step toward understanding dynamics and potential of this process. Therefore, the primary goal of this work is to develop a computer model that will support investigations of both viral and non-viral methods for gene transfer on regenerative tissues including genetically modified stem cells. Different simulation scenarios were implemented, and their results were encouraging compared to ex-vivo experiments, where, the error rate lies in the range of acceptable values in this domain of application
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References
A.L. David, D. Peebles, Gene therapy for the fetus: is there a future? Best Pract. Res. Clin. Obstet. Gynaecol. 22(1), 203–218 (2008)
M. Baker, (2007-12-06). Adult cells reprogrammed to pluripotency, without tumors. Nature Reports Stem Cells. Retrieved on 2007-12-11
R.S. Oliveri. Epigenetic dedifferentiation of somatic cells into pluripotency: cellular alchemy in the age of regenerative medicine? Regen. Med. 2(5), 795–816 (Sept 2007)
P. Collas, C.K. Taranger, Toward reprogramming cells to pluripotency. Ernst Schering Res Found Workshop. 2006;(60):47–67
J. Yu, J. Domen, D. Panchision, T.P. Zwaka, M.L. Rohrbaugh, et al. Regenerative medicine, NIH Report, 2006
A. Atala, Recent developments in tissue engineering and regenerative medicine. Curr. Opin. Pediatr. 18(2), 167–171, 2006
Y. Junying, J.A. Thomson, Regenerative Medicine, 2006
M. Colavito, M.A. Palladino, Gene Therapy, 1st edn. (Benjamin Cummings, Aug 2006) ISBN-10: 0805338195
D.V. Schaffer, W. Zhou, Gene Therapy and Gene Delivery Systems (Springer, Feb 2006) ISBN: 3540284044
A. Battler, J. Leor, Stem Cell and Gene-Based Therapy: Frontiers in Regenerative Medicine (Springer, June 2007) ISBN: 184628676X
J. Phillips, C. Gersbach, A. Garcia, Virus-based gene therapy strategies for bone regeneration. Biomaterials 28, 211–229 (2007)
Y. Yi, S.H. Hahm, KH. Lee, Retroviral gene therapy: safety issues and possible solutions. Curr. Gene Ther.5, 25–35 (2005)
L.F. Maxfield, C.D. Fraize, J.M. Coffin, Relationship between retroviral DNA-integration-site selection and host cell transcription. Proc. Natl. Acad. Sci. USA 102, 1436–41 (2005)
W. Weber, M. Fussenegger Pharmacologic transgene control systems for gene therapy. J Gene Med 8(5), 535–556 (2006)
S. Mehier-Humbert, R.H. Guy, Physical methods for gene transfer: improving the kinetics of gene delivery into cells. Adv. Drug Deliv. Rev. 57 733–753 (2005)
C. Capaccio, N.S. Stoykov, R. Sundararajan, D.A. Dean, Quantifying induced electric field strengths during gene transfer to the intact rat vasculature. J. Electrostat. 67 652–662, (2009)
J.P. Roberts, Gene therapy’s fall and rise (again). Scientist 18(18), 22–24 (2004)
U. Bastolla, M. Porto, H.E. Roman, M. Vendruscolo, Structural Approaches to Sequence Evolution: Molecules, Networks, Populations, 1 edn. (Springer, Jan 2007) ISBN-10: 3540353054
A.J. Nair, Introduction to Biotechnology and Genetic Engineering. Firewall/isp (May 2008) ISBN: 8131803392
S.J. Russell, P. Norvig, Artificial Intelligence: A Modern Approach, 2nd edn. (Prentice Hall, Upper Saddle River, NJ, 2003) ISBN: 0-13-790395-2
M. Golzio, J. Teissie, M.-P. Rols, Cell synchronization effect on mammalian cell permeabilization and gene delivery by electric field. Biochimica et Biophysica Acta 1563(2002), 23–28, (2002)
V. Sukhorukov, C. Djuzenova, W. Arnold, U. Zimmermann, J. Membr. Biol. 142 77–92 (1994)
M.P. Rols, J. Teissie, Biophys. J. 58 1089–1098 (1990)
H.A. Crissman, J.A. Steinkamp, Eur. J. Histochem. 37 (1993) 129–138, 1993
T.J. Nelson, A. Terzic Induced pluripotent stem cells: reprogrammed without a trace. Regen. Med. (2009). doi:10.2217/rme.09.16
C.A. Sommer, M. Stadtfeld, G.J. Murphy et al. Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Stem Cells (2009). doi:10.1634/stemcells.2008-1075
J. Chan, K. O’Donoghue, J. de la Fuente et al. Human fetal mesenchymal stem cells as vehicles for gene delivery. Stem Cells 23, 93–102 (2005)
N.B. Ivanova, J.T. Dimos, C. Schaniel et al. A stem cell molecular signature (Sept 2002). doi:10.1126/science.1073823
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Adly, A.S., Kandil, O.A.D., Ibrahim, M.S., Adly, M.S., Adly, A.S., Adly, A.S. (2010). Computational and Theoretical Concepts for Regulating Stem Cells Using Viral and Physical Methods. In: Ao, SI., Rieger, B., Amouzegar, M. (eds) Machine Learning and Systems Engineering. Lecture Notes in Electrical Engineering, vol 68. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9419-3_41
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DOI: https://doi.org/10.1007/978-90-481-9419-3_41
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