Abstract
MicroRNAs form a novel class of functional biomolecules with a great potential to be used as informative biomarkers for clinical diagnostics such as for Alzheimer’s disease (AD). For this purpose, we tested the prerequisite: can microRNAs be isolated and quantified from the spinal fluid of patients at risk for or diagnosed with Alzheimer disease? Our data show that quantitative analysis of all currently known microRNA’s (n = 667) is technically possible in spinal fluid of patients with Alzheimer disease. For this, we applied the Megaplex protocol with Taqman Array MicroRNA cards on small RNA isolated with the MirVana Paris kit.
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Abbreviations
- AD:
-
Alzheimer’s disease
- CSF:
-
Cerebrospinal fluid
- qRT-PCR:
-
Quantitative reverse transcriptase PCR
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van Harten, A. et al. (2010). MicroRNA Analysis in the Spinal Fluid of Alzheimer Patients: A Methodological Feasibility Study. In: Gahan, P. (eds) Circulating Nucleic Acids in Plasma and Serum. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9382-0_37
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DOI: https://doi.org/10.1007/978-90-481-9382-0_37
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