Abstract
Following exposure to X-radiation (10 cGy), human mesenchymal stem cells (MSCs) appear to exhibit transposition of 1q12 loci of the homologous chromosomes from the perimembrane regions deep into the nucleus, their approximation and activation of chromosomal nucleolus-forming regions – the initial stages of the adaptive response (AR) development. Transfer of the culture medium containing irradiated MSCs to the intact bystander cells induced in the latter all three reactions being studied, i.e., the bystander effect (BE). Previously, similar results concerning radiation-induced AR and BE were obtained for human G0-lymphocytes. The cumulative findings obtained suggest a universal nature of the chromosomal-homolog loci-transposition reaction revealed during the development of AR in the differentiated (lymphocytes) or undifferentiated (MSCs) human cells, and the possibility of the development of the radiation-induced BE in both suspended and monolayer-cell cultures during transfer of stress-signalling factors through the incubation medium. We believe that these factors are the fragments of extracellular genomic DNA of apoptotic cells.
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Abbreviations
- AR:
-
Adaptive response
- BE:
-
Bystander effect
- ecDNA:
-
Extracellular DNA
- ECM:
-
Exposed-cell medium
- FISH:
-
Fluorescent in situ hybridization
- GAPDH:
-
Glyceraldehyde-3-phosphate-dehydrogenase
- ICM:
-
Intact-cell medium
- MSCs:
-
Mesenchymal stem cells
- MyD88:
-
Myeloid differentiation primary response protein
- NFRs:
-
Nucleolar-forming regions
- PCR:
-
Polymerase chain reaction
- SS:
-
Stress signalling
- TLR9:
-
Toll-like receptor 9
References
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Ermakov, A.V. et al. (2010). Development of the Adaptive Response and Bystander Effect Induced by Low-Dose Ionising Radiation in Human Mesenchymal Stem Cells. In: Gahan, P. (eds) Circulating Nucleic Acids in Plasma and Serum. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9382-0_31
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DOI: https://doi.org/10.1007/978-90-481-9382-0_31
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