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Lectin Microarrays: Simple Tools for the Analysis of Complex Glycans

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Functional and Structural Proteomics of Glycoproteins

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Abstract

The emerging roles for post-translational modifications in the regulation of cellular function have turned the spotlight on glycosylation. Given the prevalence of protein and lipid glycosylation, it has become imperative to create and utilize new tools to study these critical biopolymers. In particular, there has been an emphasis on the development of high-throughput methodologies to study the structural and functional aspects of glycan-protein interactions. The use of carbohydrate binding proteins (i.e. lectins) in a microarray format has greatly enhanced our ability to de-convolute the structural aspects of the glycome. This simple and unique technology provides a rapid method for glycomic analysis, which opens up the field of glycobiology to more systems-based approaches towards function.

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Abbreviations

CHO:

Chinese hamster ovary

DC-SIGN:

dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin

Fuc:

fucose

Gal:

galactose

GlcNAc:

N-acetylglucosamine

HIV:

human immunodeficiency virus

LacNAc:

N-acetyllactosamine

NHS:

N-hydroxysuccinimidyl

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Acknowledgements

L.K.M would like to acknowledge the Alfred P. Sloan foundation for funding.

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Authors and Affiliations

  1. Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY, 10003, USA

    Lakshmi Krishnamoorthy & Lara K. Mahal

Authors
  1. Lakshmi Krishnamoorthy
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  2. Lara K. Mahal
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Correspondence to Lara K. Mahal .

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Editors and Affiliations

  1. Oxford Protein Production Facility, Roosevelt DR, Oxford, OX3 7BN, United Kingdom

    Raymond Owens

  2. Oxford Protein Production Facility, Roosevelt Dr, Oxford, OX3 7BN, United Kingdom

    Joanne Nettleship

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Krishnamoorthy, L., Mahal, L.K. (2010). Lectin Microarrays: Simple Tools for the Analysis of Complex Glycans. In: Owens, R., Nettleship, J. (eds) Functional and Structural Proteomics of Glycoproteins. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9355-4_4

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