Abstract
High-pressure molecular biophysics is a developing field for three main reasons. Pressure has a unique potential, in particular for the exploration of the energy landscape of biomolecules. Progress in instrumentation has extended the range of biophysical techniques under pressure and often relaxed technical constraints on sample confinement. Two high-resolution structural methods are now available at high pressure, NMR and macromolecular crystallography (HPMX). We describe materials and methods of HPMX, now a full-fledged technique taking advantage of purposely-built diamond-anvil cells, ultra-short wavelength synchrotron radiation and improved crystal-loading procedures.
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References
Akasaka, K., 2006, Probing conformational fluctuations of proteins by pressure perturbation, Chem. Rev. 106:1814–1835.
Anfinsen, C.B., 1973, Principles that govern the folding of protein chains, Science 181:223–230.
Balny, C., Hayashi, R., Heremans, K., and Masson, P. (ed.), 1992,High pressure and biotechnology, Colloque INSERM 224, John Libbey, London.
Boehler, R., and de Hantsetters, K., 2004, New anvil designs in diamond-cells, High Press. Res. 24:1–6.
Bridgman, P.W., 1914, The coagulation of albumin by pressure, J. Biol. Chem. 19:511–512.
Forman, R.A., Piermarini, G.J., Barnett, J.D., and Block, S., 1972, Pressure measurement made by the utilization of ruby sharp-line luminescence, Science 176:284–285.
Fourme R., 1968, Appareillage pour études radiocristallographiques sous pression et à température variable, J. Appl. Cryst. 1:23–29.
Fourme, R., Girard, E., Kahn, R., Ascone, I., Mezouar, M., Dhaussy A.C., Lin, T.W., and Johnson, J.E., 2003, Using a quasi-parallel X-ray beam of ultra-short wavelength for high-pressure virus crystallography: implications for standard macromolecular crystallography, Acta Cryst.D 59:1767–1772.
Fourme, R., Girard, E., Kahn, R., Dhaussy, A.-C., and Ascone, I., 2009, Advances in high-pressure biophysics: status and prospects of macromolecular crystallography, Ann. Rev. Biophys. 38:153–171.
Fourme, R., Kahn, R., Mezouar, M., Girard, E., Hoerentrup, C., Prange, C., Ascone, I., 2001, High pressure protein crystallography (HPPX): instrumentation, methodology and results of data collection on lysozyme crystals, J. Synchrotron Rad. 8:1149–1156.
Girard, E., Dhaussy, A.-C., Couzinet, B., Chervin, J.C., Mezouar, M., Kahn, R., Ascone, I., and Fourme, R., 2007, Toward full-fledged high-pressure macromolecular crystallography (HPMX), J. Appl. Cryst. 40:912–918.
Girard, E., Fourme, R., Ciurko, R., Joly, J., Bouis, F., Legrand, P., Jacobs, J., Dhaussy, A.-C., Ferrer, J.-L., Mezouar, M. and Kahn, R., 2010, Macromolecular crystallography at high pressure with pneumatic diamond-anvil cells handled by a six-axis robotic arm, J. Appl. Cryst. 43: xxx–xxx.
Girard, E., Prangé, T., Dhaussy, A.-C., Migianu, E., Lecouvey, M., Chervin, J.-C., Mezouar, M., Kahn, R., Fourme, R., 2007, Adaptation of base-paired double-helix to extreme hydrostatic pressure, Nucl. Acids Res. 35(14):4800–4808.
Gross, M., and Jaenicke, R., 1994, The influence of high hydrostatic pressure on structure, function and assembly of proteins and protein complexes, Eur. J. Biochem. 221:617–630.
Horikoshi, K., and Grant, W.D., 1998, Extremophiles. Microbial life in extreme environments, Wiley-Liss, New York.
Kabsch, W., 1993, Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants, J. Appl. Cryst. 26:795–800.
Katrusiak, A., and Dauter, Z., 1996, Compressibility of lysozyme crystals by X-ray diffraction, Acta Cryst. D 52:607–608.
Kundrot, C.E., and Richards, F.M., 1987, Crystal structure of hen egg-white lysozyme at a hydrostatic pressure of 1000 atmospheres, J. Mol. Biol. 193:157–170.
Le Toullec, R., Pinceaux, J.-P., and Loubeyre, P., 1988, The membrane diamond anvil cell: a new device for generating continuous pressure and temperature variations, High Press. Res. 1:77–90.
Merrill, L.L., and Bassett, W.A., 1974, Miniature diamond anvil pressure cell for single crystal X-ray diffraction studies, Rev. Sci. Instrum. 45:290–294.
Mezouar, M., Crichton, W.A., Bauchau, S., Thurel, F., Witsch, H., Torrecillas, F, Blattmann, G., Marion, P., Dabin, Y., Chavanne, J., Hignette, O., Morawe, C., and Borel, C., 2005, Development of a new state-of-the-art beamline optimized for monochromatic single-crystal and powder X-ray diffraction under extreme conditions at the ESRF, J. Synchrotron. Rad. 12:659–664.
Mozhaev, V.V., Heremans, K., Franks, J., Masson, P., and Balny, C., 1994, Exploiting the effect of high hydrostatic pressure in biotechnological applications, Trends Biotechnol. 12:493–501.
Van Valkenburg A., 1962, Visual observations of high pressure transitions, Rev. Sci. Instrum. 33:1462.
Weber, G., 1992, Protein interactions, Chapman & Hall, New York, pp. 211–214.
Weber, G., 1992, Thermodynamics of the association and the pressure dissociation of oligomeric proteins, J. Phys. Chem. 97:7108–7115.
Weir, C.E., Lippincott, E.R., Van Valkenburg, A., Bunting, E.N., 1959, Infrared studies in the 1-micron to 15-micron region to 30,000 atmospheres, J. Res. Natl. Bur. Stand. – Phys. Chem. 63A:55–62.
Yayanos, A.A., and Delong, E.F., 1987, Deep-sea bacterial fitness to environmental temperatures and pressures. In: Current perspectives in high pressure biology, edited by H.W. Jannasch et al., Academic Press, London, pp. 17–32.
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Fourme, R., Ascone, I., Mezouar, M., Dhaussy, AC., Kahn, R., Girard, E. (2010). High-Pressure Crystallography of Biomolecules: Recent Achievements. I – Introduction, Materials and Methods. In: Boldyreva, E., Dera, P. (eds) High-Pressure Crystallography. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9258-8_48
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