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Endotoxins: Structure, Function and Recognition pp 27–51Cite as

Purification and Characterization of Lipopolysaccharides

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Part of the book series: Subcellular Biochemistry ((SCBI,volume 53))

Abstract

Lipopolysaccharides are the major components on the surface of most Gram-negative bacteria, and recognized by immune cells as a pathogen-associated molecule. They can cause severe diseases like sepsis and therefore known as endotoxins. Lipopolysaccharide consists of lipid A, core oligosaccharide and O-antigen repeats. Lipid A is responsible for the major bioactivity of endotoxin. Because of their specific structure and amphipathic property, purification and analysis of lipopolysaccharides are difficult. In this chapter, we summarize the available approaches for extraction, purification and analysis of lipopolysaccharides.

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Abbreviations

LPS:

lipopolysaccharide

R-LPS:

rough type LPS

S-LPS:

smooth type LPS

PCP:

phenol, chloroform and petroleum ether

RNase:

ribonuclease

DNase I:

deoxyribonuclease I

SDS:

sodium dodecyl sulfate

SDS-PAGE:

sodium dodecyl sulfate-polyacylamide gel electrophoresis

DOC:

deoxycholate

CE:

capillary electrophoresis

FT-ICR MS:

Fourier-transform ion cyclotron resonance mass spectrometer

ESI MS:

electrospray ionization mass spectrometry

EtBr:

ethidium bromide

TAE:

triethylamine

TLR2:

Toll-like receptor 2

TLR4:

Toll-like receptor 4

TLC:

thin layer chromatography

CFU:

colony forming unit

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Acknowledgements

Funding was provided by grants from the National Natural Science Foundation of China (NSFC 30770114, NSFC30870074), the Program of State Key Laboratory of Food Science and Technology (SKLF-MB-200801), the 111 project (111-2-06), the Basic Research Programs of Jiangsu Province (BK2009003).

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  1. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China

    Xiaoyuan Wang, Chan Zhang, Feng Shi & Xiaoqing Hu

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  1. Xiaoyuan Wang
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  2. Chan Zhang
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  3. Feng Shi
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  4. Xiaoqing Hu
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Correspondence to Xiaoyuan Wang .

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  1. Dept. Biochemistry, Jiangnan University, Wuxi, Jiangsu, 214122, China, People's Republic

    Xiaoyuan Wang

  2. European Southern Observatory (ESO), Karl-Schwarzschild-Str. 2, Garching, 85748, Germany

    Peter J. Quinn

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Wang, X., Zhang, C., Shi, F., Hu, X. (2010). Purification and Characterization of Lipopolysaccharides. In: Wang, X., Quinn, P. (eds) Endotoxins: Structure, Function and Recognition. Subcellular Biochemistry, vol 53. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9078-2_2

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