Abstract
The Flaviviruses responsible for most cases of encephalitis in Australia and South East Asia include Murray Valley Encephalitis virus, Kunjin virus, Japanese B Encephalitis virus and West Nile virus. Flavivirus infection is usually diagnosed by the detection of virus-specific IgM antibody or by measuring a rising titre of IgG antibodies in the acute phase of illness. Local IgM antibody can also be detected in the CSF of patients with CNS disease. Because many infections with flaviviruses are inapparent, caution should be exercised in the interpretation of serological results. Patients may also have viral RNA in the blood or CSF and its presence constitutes a definite indicator of current infection.
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References
Jalal H, Stephen H, Curran MD et al (2006) Development and validation of a RotorGene real-time PCR assay for detection, identification and quantification of Chlamydia trachomatis in a single reaction. J Clin Microbiol 44:206–213
Stanley KK, Szewezuk SA (2005) Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acid Res 33:e180
Sykes PJ, Neoh MJ, Hughes E et al (1992) Quantitation of targets for PCR by use of limiting dilution. Biotechniques 13:444–449
Tanaka M (1993) Rapid identification of flaviviruses using the polymerase chain reaction. J Virol Methods 41:311–322
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We would like to thank Eileen J. Pratt for valuable technical assistance provided.
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Harnett, G.B., Cattell, J.A. (2010). Flavivirus. In: Schuller, M., Sloots, T., James, G., Halliday, C., Carter, I. (eds) PCR for Clinical Microbiology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9039-3_34
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DOI: https://doi.org/10.1007/978-90-481-9039-3_34
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