Abstract
The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the intervening variable regions. The variable portions of the 16S rRNA gene provide unique signatures that can be analyzed to provide an identification of the bacteria species in the sample.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Clinical and Laboratory Standards Institute (2007) Interpretive criteria for microorganism identification by DNA target sequencing; proposed guideline. 27(22):MP18-P
Drancourt M, Raoult D (2005) Sequence based identification of new bacteria: a proposition for creation of an orphan bacterium repository. J Clin Microbiol 43:4311–4315
Relman DA (1993) Universal bacterial 16S rRNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ (eds) Diagnostic Molecular Biology, Principles and Applications. Mayo Foundation Rochester, MN, USA, pp 489–495
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
James, G. (2010). Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene. In: Schuller, M., Sloots, T., James, G., Halliday, C., Carter, I. (eds) PCR for Clinical Microbiology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9039-3_28
Download citation
DOI: https://doi.org/10.1007/978-90-481-9039-3_28
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-9038-6
Online ISBN: 978-90-481-9039-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)