Abstract
Fine-leaved fescue (Festuca L.) species are valuable breeding material with potential use for marginal land cover. However, the breeding of these species is complicated by morphological similarity among species and high heterogenity within species. Molecular DNA markers are needed to facilitate species identification. Inter simple sequence repeat polymerase chain reaction (ISSR-PCR) was used for the genetic analysis of the five fine-leaved fescue species encountered in Lithuania, namely Festuca sabulosa, Festuca polesica, Festuca ovina, Festuca trachyphylla and Festuca psammophila. Fifty six ISSR markers were scored for 21 fescue accessions. Similarity indicies were calculated and UPGMA dendrogram constructed with NTSYSpc 2.2. Three species (F. ovina, F. trachyphylla and F. psammophila) could be distinguished as separate clusters in the dendrogram. Further analysis revealed two ISSR fragments of 600 and 950 bp to be species-specific for F. psammophila. These fragments are potential targets for more specific SCAR marker development. The remaining two highly related species (F. sabulosa and F. polesica) formed one intermixed cluster and could not be distinguished from each other. The results of this work show the suitability of the ISSR method to investigate genetic diversity of fine-leaved fescue species encountered in Lithuania and provide valuable data for the identification of these species.
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Acknowledgements
This work was partly supported by the Lithuanian Science Council Student Research Fellowship Award (RA) and by the Lithuanian State Science and Studies Foundation (grant N21/2008).
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Armonienė, R., Stukonis, V., Paplauskienė, V., Brazauskas, G. (2010). The Genetic Diversity of Fine-Leaved Fescue (Festuca L.) Species in Lithuania. In: Huyghe, C. (eds) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8706-5_4
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DOI: https://doi.org/10.1007/978-90-481-8706-5_4
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