Abstract
Festulolium are intergeneric hybrids developed to combine the agronomic advantages of ryegrass (Lolium) with the persistence and abiotic stress tolerance of fescues (Festuca). To survey genetic evolution of tetraploid Festulolium populations bred from F. glaucescens (Fg) × L. multiflorum (Lm) cross, we examined 47 EST-SSR and STS public markers for: their amplification efficiency in Fg and Lm; the genome specificity of their PCR products; and ability for high throughput genotyping (band size ≤ 500 bp). A total of 322 bands were scored among which 93 were Fg-specific, 173, Lm-specific and 56 common to both species. F. arundinacea originating EST-SSR amplified Fg DNA at a significant higher rate than L. perenne EST-SSR and showed a significant higher number of Fg-specific bands. In fine, we selected 12 markers among which two primer pairs need to be redefined for use in ABI sequencer. We also present the genotypic distribution of 7 markers in a population of tetraploid Lm × Fg F1 hybrids.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Doyle, J.J., Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bul. 19:11–15.
Ghesquière, M., Emile, J.C., Jadas-Hécart, J., et al. 1996. First in vivo assesment of feeding value of Festulolium hybrids derived from Festuca arundinacea var. glaucescens and selection for palatability. Plant Breed. 115:238–244.
Kopecky, D., Loureiro, J., Zwierzykowski, Z., Ghesquiere, M., Dolezel, J. 2006. Genome constitution and evolution in Lolium × Festuca hybrid cultivars (Festulolium). Theor. Appl. Genet. 113:731–742.
Lem, P., Lallemand, J. 2003. Grass consensus STS markers: An efficient approach for detecting polymorphism in Lolium. Theor. Appl. Genet. 107:1113–1122.
Mian, M., Saha, M., Hopkins, A., Wang, Z. 2005. Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses. Genome. 48:637–647.
Saha, M., Mian, M., Eujayl, I., et al. 2004. Tall fescue EST-SSR markers with transferability across several grass species. Theor. Appl. Genet. 109:781–791.
Studer, B., Asp, T., Frei, U., et al. 2008. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Mol. Breeding. 21:533–548.
Zwierzykowski, Z., Kosmala, A., Zwierzykowska, E., et al. 2006. Genome balance in six successive generations of the allotetraploid Festuca pratensis x Lolium perenne. Theor. Appl. Genet. 113:539–547.
Acknowledgments
The authors would like to thank all colleagues who contributed to this study and La Région Poitou-Charentes for its financial support.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media B.V.
About this paper
Cite this paper
Missi-Bakour, N., Durand, JL., Ghesquière, M. (2010). Test and Selection of Festuca/Lolium Specific Markers for Festulolium Genetic Investigations. In: Huyghe, C. (eds) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8706-5_34
Download citation
DOI: https://doi.org/10.1007/978-90-481-8706-5_34
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-8705-8
Online ISBN: 978-90-481-8706-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)