Abstract
Primary malignant brain tumors are highly lethal cancers and vary in their histology, due to the many cell types in the brain and also the multipotent cells of origin (Singh et al. 2003, 2004). The most notorious among these is glioblastoma multiforme (GBM), the most aggressive glioma subtype, carrying the highest World Health Organization (WHO) grade of IV. As the name suggests, GBM can have features of several cell types, which can be regionally distinct, depending on the evolution of the tumor from CNS precursor cells. The varied cellular composition of these tumors can give rise to differences in phenotype, which alter therapeutic response. To better characterize the varied molecular phenotype of malignant brain tumors, we devised methodology which utilizes preoperative MR imaging to define areas of interest for stereotactic biopsy, image guided biopsy, followed by nucleic acid extraction with an “in-process” histopathological scoring during tissue processing. The procedure is followed by molecular phenotyping using any highly reliable oligonucleotide microarray platform for molecular characterization. We have used Affymetrix gene expression microarrays due to the high reproducibility of these assays. The global gene expression profiling approach also allows a highly robust analysis to be performed on individual tumors, revealing a much more complete assessment of the overall molecular phenotype.
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Van Meter, T.E., Tye, G., Dumur, C., Broaddus, W.C. (2011). Assessment of Heterogeneity in Malignant Brain Tumors. In: Hayat, M. (eds) Methods of Cancer Diagnosis, Therapy, and Prognosis. Methods of Cancer Diagnosis, Therapy and Prognosis, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8665-5_3
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DOI: https://doi.org/10.1007/978-90-481-8665-5_3
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