Abstract
The haptophyte Isochrysis galbana is widely used as a food source for bivalve aquaculture as it is rich in fatty acid. In this study, the effect of different colors of intermittent light on the multiplication of cells of I. galbana and their fatty acid composition was investigated. I. galbana was cultivated under conditions of white, blue (dominant wavelength; 470 nm), green (525 nm), or red (660 nm) intermittent light (frequency; 10,000 Hz, duty ratio; 50%, light intensity; 52 μmol photons m−2 s−1, water temperature; 20°C).
The concentration of cells and fatty acid composition were measured at 6 days after the beginning of cultivation.
Cell concentration of I. galbana increased with time. The cell concentration on the 6th day under the blue light condition was significantly higher than in other light conditions. The numbers of cells under white, red, and green light were 65%, 60%, and 40% of the blue light condition, respectively.
I. galbana contains mostly lipids of 16:0, 16:1n-7, and 20:5n-3 (EPA), which account for approximately 50% of the total lipids.
In conclusion, the number of cells on the 6th day under the blue intermittent light was approximately double that under white continuous light, and therefore the amount of fatty acids available is also considered to be approximately double.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
AOCS Official Method Ce 1b-89 (1989) Official method for marine oil fatty acid composition by GLC. In: Official methods and recommended practices of the American Oil Chemists’ Society, 4th ed. AOCS Press, Campaign, IL
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Guillard RR, Ryther L (1962) Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can J Microbiol 8:229–239
Hochberg Y (1988) A sharper Bonferonni procedure for multiple tests of significance. Biometrika 75:800–803
Phatarpekar PV, Sreepada RA, Pednekar C, Achuthankutty CT (2000) A comparative study on growth performance and biochemical composition of mixed culture of Isochrysis galbana and Chaetoceros calsitrans with monocultures. Aquaculture 181: 141–155
Toro JE (1989) The growth rate of two species of microalgae used in shellfish hatcheries cultured under two light regimes. Aquac Fish Manage 20:249–254
Tzovenis I, Pauw ND, Sorgeloos P (2003) Optimisation of T-ISO biomass production rich in essential fatty acids. I. Effect of different light regimes on growth and biomass production. Aquaculture 216:203–222
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media B.V.
About this paper
Cite this paper
Yago, T., Arakawa, H., Morinaga, T., Yoshie-Stark, Y., Yoshioka, M. (2010). Effect of Wavelength of Intermittent Light on the Growth and Fatty Acid Profile of the Haptophyte Isochrysis galbana . In: Ceccaldi, HJ., Dekeyser, I., Girault, M., Stora, G. (eds) Global Change: Mankind-Marine Environment Interactions. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8630-3_8
Download citation
DOI: https://doi.org/10.1007/978-90-481-8630-3_8
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-8629-7
Online ISBN: 978-90-481-8630-3
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)