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Detection and Quantification of Bacteria and Fungi Using Solid-Phase Cytometry

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Detection of Bacteria, Viruses, Parasites and Fungi

Abstract

Solid-phase cytometry (SPC) was developed to meet the demand for fast and sensitive microbial detection and quantification methods. By combining the principles of epifluorescence microscopy and flow cytometry, this technique allows accurate, fast and automated detection of single microbial cells. SPC analysis is a five-step procedure, including membrane filtration, fluorescent labelling of the retained cells, scanning of the membrane filter, data analysis by a computer and microscopic validation. The aim of this review is to present the basic principles of SPC, its advantages and disadvantages and to discuss the existing applications as well as some perspectives for future research.

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Acknowledgement

The research of Lies Vanhee was financially supported by the Bijzonder Onderzoeksfonds of Ghent University (project B/07601/02).

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Authors and Affiliations

  1. Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Lies ME Vanhee, Eva D’Haese, Ils Cools, Hans J Nelis & Tom Coenye

  2. Health Care Department, National Institute for Health and Disablement Insurance, Brussels, Belgium

    Eva D’Haese

  3. GULLIMEX BV, Erpe-Mere, Belgium

    Ils Cools

Authors
  1. Lies ME Vanhee
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  2. Eva D’Haese
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  3. Ils Cools
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  4. Hans J Nelis
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  5. Tom Coenye
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Corresponding author

Correspondence to Tom Coenye .

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Editors and Affiliations

  1. Policlinico Monteluce, Dipto. Medicina Clinica e Sperimentale, UniversitĂ  di Perugia, Perugia, 06122, Italy

    Mariapia Viola Magni

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Vanhee, L.M., D’Haese, E., Cools, I., Nelis, H.J., Coenye, T. (2010). Detection and Quantification of Bacteria and Fungi Using Solid-Phase Cytometry. In: Viola Magni, M. (eds) Detection of Bacteria, Viruses, Parasites and Fungi. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8544-3_2

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