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Reversing Age-Related DNA Damage Through Engineered DNA Repair

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The Future of Aging

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Abstract

The last two decades have shown significant advances in our understanding of age- and disease-related alterations in the regulation of gene expression and the underlying endogenous gene repair pathways. As a result, there have been some important strides in the development of engineered technologies for repairing mutated or damaged DNA via the development, delivery, and integration of specific and selective modified DNA into precise locations. This chapter describes available strategies for engineered gene repair including homologous recombination, the use of ribozymes, antisense nucleotides and DNA ribonucleases, single strand replacement/ chimeraplasty, and triplex DNA. The evolution of these approaches together with RNA interference is discussed, and relevant mechanisms and pathways are described. Present demonstrations of the utility of each of these gene modification approaches for therapeutic use imply that these methods can be employed to correct inherited and aging-related mutations and their consequences in future applications.

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Authors and Affiliations

  1. Department of Medicine, VFW Cancer Research Center, University of Minnesota Medical School, 406 Harvard Street SE, Minneapolis, MN, USA

    Clifford J. Steer

  2. Department of Medicine, University of Minnesota Medical School, 420 Delaware Street SE, MMC 36, Minneapolis, MN, USA

    Betsy T. Kren

Authors
  1. Clifford J. Steer
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  2. Betsy T. Kren
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Correspondence to Clifford J. Steer .

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Editors and Affiliations

  1. Intervene Biomedical, LLC, Box 478, Norco, CA, 92860, USA

    Gregory M. Fahy

  2. BioTime, Inc., 1301 Harbor Bay Parkway, Alameda, CA, 94502, USA

    Michael D. West

  3. Institute of Molecular Biology Department of Chemistry and Biochemistry Young Hall, University of California, Los Angeles, CA, 90095-1569, USA

    L. Stephen Coles

  4. Critical Care Research, Inc., 10743 Civic Center Drive, Rancho Cucamonga, CA, 91730, USA

    Steven B. Harris

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Steer, C.J., Kren, B.T. (2010). Reversing Age-Related DNA Damage Through Engineered DNA Repair. In: Fahy, G.M., West, M.D., Coles, L.S., Harris, S.B. (eds) The Future of Aging. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3999-6_21

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