Key Points
Major quality issues of molecular cytogenetics are:
• Manpower: Well-trained personal, knowing chromosomes on GTG-band level and pitfalls of FISH-techniques are a must.
• Equipment: All laboratory equipment has to be available in double, to guarantee continuous work in case of damage of one machine.
• Microscope: The maximum time of operation of fluorescence microscope lamps and filters have to be observed.
• Probes and samples: Protocols with clear instructions for handling/ work-up of DNA-probes and tissue samples must be adapted by each user to the specific requirements.
• Evaluation and reporting: While evaluation can be done by technical staff, the final approval of the result and the reporting has to be done by a correspondingly qualified PhD and/or MD.
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References
Gebhart E, Trautmann U, Reichardt S, et al. Chromosomal heterogeneity of aneuploid leukemic cell populations detected by conventional karyotyping and by fluorescence in situ hybridization (FISH). Anticancer Res 1993; 13: 1857–1862.
ISCN 2005. An International System for Human Cytogenetics Nomenclature (2005). L.G. Shaffer, N. Tommerup (eds). Basel: Karger, 2005.
ISCN 2009. An International System for Human Cytogenetics Nomenclature (2009). L.G. Shaffer, M.L. Slovak, L. Campbell (eds). Basel: Karger, 2009.
Liehr T. Multicolor fluorescence in situ hybridization (mFISH) literature, 2009a. http://www.med.uni-jena.de/fish/mFISH/mFISHlit.htm.
Liehr T Fluorescence in situ Hybridization (FISH) – Application Guide. Springer, Chapter 7 and 8, 75–98, 2009b.
Liehr T, Ziegler M. Rapid prenatal diagnostics in the interphase nucleus: procedure and cut-off rates. J Histochem Cytochem 2005; 53: 289–291.
Mitelman F, Rowley JD. ISCN (2005) is not acceptable for describing clonal evolution in cancer. Genes Chromosomes Cancer 2007; 46: 213–214.
Pinkel D, Straume T, Gray JW. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 1986; 83: 2934–2938.
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Liehr, T. (2010). Fluorescence In Situ Hybridization (FISH) – Quality Issues in Molecular Cytogenetics. In: Kristoffersson, U., Schmidtke, J., Cassiman, J. (eds) Quality Issues in Clinical Genetic Services. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3919-4_32
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DOI: https://doi.org/10.1007/978-90-481-3919-4_32
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