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Animal Cell Technology: Basic & Applied Aspects pp 51–55Cite as

Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines

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Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

Abstract

We developed that induced with a sodium butyrate mass production of cell-size vesicles from several human lymphoma cells. Ramos, a human burkitt lymphoma was the best induction rate among several human cell lines. However, all cells were dead after NaB treatment. The trypan blue dye staining showed the leakless of the vesicle. Therefore, it is suggested that the vesicle could be available for a drug carrier. The immunostaining analysis showed the retainment of surface antigen such as IgM, implying the possibility of specific vesicle production by transfection of desired gene into viable cells followed by the NaB treatment. This study is applicable to membrane protein isolation, drug delivery system, and microreactor development.

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References

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Author information

Authors and Affiliations

  1. Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu-shi, Fukuoka, 808-0196, Japan

    Makoto Yamanaka & Takashi Yasuda

  2. The Cell Engineering Center, Kitakyushu National College of Technology, 802-0985, Kitakyushu-shi, Fukuoka, Japan

    Shigeki Nakamura, Aiko Inoue, Yuichi Inoue & Hiroharu Kawahara

  3. The Cell Engineering Center, Kitakyushu National College of Technology, Kokuraminami-ku, 802-0985, Kitakyushu, Japan

    Aiko Inoue & Yuichi Inoue

Authors
  1. Makoto Yamanaka
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  2. Shigeki Nakamura
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  3. Aiko Inoue
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  4. Takashi Yasuda
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  5. Yuichi Inoue
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  6. Hiroharu Kawahara
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Corresponding author

Correspondence to Makoto Yamanaka .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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Cite this paper

Yamanaka, M., Nakamura, S., Inoue, A., Yasuda, T., Inoue, Y., Kawahara, H. (2010). Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_9

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