Abstract
A vascularized tissue construction approach is promising for large-scale tissue engineering. Regular alignment of endothelial cells within 3D tissue is a key technique for vascular network construction. We focused on endothelial cell-covered spheroids of 100 μm in diameter and their integration. Such a spheroid is a candidate tissue unit for the initial alignment of endothelial cells at regular 100 μm intervals within tissue. To form vascularized tissue, we packed endothelial cell-covered spheroids into the internal space of hollow fibers. The spatial distribution of the endothelial cells was then investigated.
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Acknowledgements
This study was supported in part by a Grant-in-Aid for Scientific Research (B) 19360375 from the Japan Society for the Promotion of Science and supported by a grant from the Global-Centre of Excellence in Novel Carbon Resource Sciences, Kyushu University.
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Inamori, M., Mizumoto, H., Kajiwara, T. (2010). Integration of Endothelial Cell-Covered Hepatocyte Spheroids for Construction of Vascularized Liver Tissue. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_8
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DOI: https://doi.org/10.1007/978-90-481-3892-0_8
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