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Animal Cell Technology: Basic & Applied Aspects pp 285–287Cite as

Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis

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Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

Abstract

In our attempt to find antimelanogenesis agents from natural sources, we determined the effect of novel compounds from Thymelaea hirsuta on melanogenesis in B16 murine melanoma cells. Cytotoxicity assay was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, after which melanin content was quantified. Results of MTT assay, using 0.1 μM up to 2 μM of the compounds, showed that the compounds are not cytotoxic. The compounds, at 0.1 μM concentration, significantly reduced the melanin content. The effect of treatment with the novel compounds generated the same effect as treatment with 100 μM arbutin a known depigmenting agent. The mechanism of melanogenesis inhibition will be looked at in our future experiments.

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Author information

Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Myra O. Villareal, Yusaku Miyamae, Junkyu Han, Hideyuki Shigemori & Hiroko Isoda

  2. Alliance for Research on North Africa, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan

    Yamada Parida & Hiroko Isoda

Authors
  1. Myra O. Villareal
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  2. Yusaku Miyamae
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  3. Junkyu Han
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  4. Yamada Parida
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  5. Hideyuki Shigemori
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  6. Hiroko Isoda
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Corresponding author

Correspondence to Myra O. Villareal .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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© 2010 Springer Science+Business Media B.V.

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Villareal, M.O., Miyamae, Y., Han, J., Parida, Y., Shigemori, H., Isoda, H. (2010). Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_47

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