Abstract
In recent years, the peptide immunotherapy using dominant T-cell epitopes has been expected as an effective treatment for allergic diseases such as Japanese cedar pollen allergy. To generate genetically manipulated chickens expressing T-cell epitope peptides of Japanese cedar pollen allergens, we constructed retroviral vector plasmids encoding an expression cassette of seven dominant human T-cell epitopes derived from the Japanese cedar pollen allergens (7crp) fused with the Fc region of immunoglobulin A or G, or chicken lysozyme. Retroviral vectors produced using the plasmids were concentrated by ultracentrifugation and injected into developing chicken embryos. The manipulated embryos were cultured to hatch with 44% hatchability. The eggs produced by the genetically manipulated chickens may be used as an oral drug for Japanese cedar pollen allergy.
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Kawabe, Y., Hayashida, Y., Numata, K., Hishigae, A., Ito, A., Kamihira, M. (2010). Production of Therapeutic Proteins Composed of Seven Dominant Human T Cell Epitopes Derived from the Japanese Cedar Pollen Allergens. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_34
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DOI: https://doi.org/10.1007/978-90-481-3892-0_34
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