Abstract
Abstract Transgenic avian bioreactors have been expected as the next generation production system for pharmaceutical proteins. When the target proteins have physiological effects on chickens, they should be produced only in the oviduct where egg white proteins such as ovalbumin and conalbumin are produced. In this study, we developed an oviduct-specific promoter system in which the tTA-TRE synthetic promoter system was combined with oviduct-specific promoters to realize the oviduct-specific and high-level expression of a target gene. When a target protein is expressed under the control of TRE promoter, the abundant level of tTA proteins promotes the high-level expression of target protein. To suppress leak expression of tTA and target genes, a synthetic promoter consisting of TRE and TATA-box neighboring regulatory regions derived from ovalbumin and conalbumin was constructed. Using this promoter, the cell-specific expression of target genes was confirmed in the primary culture of oviduct cells. Since the synthetic promoter system is compact in size compared with the original ovalbumin and conalbumin promoters, the system may be useful to construct oviduct-specific expression vectors for generating transgenic avian bioreactors.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Rudolph, N.S., (1999) Biopharmaceutical production in transgenic livestock. Trends. Biotechnol. 17: 367–374.
Kues, W.A., and Niemann, H. (2004) The contribution of farm animals to human health. Trends. Biotechnol. 22: 286–294.
Sang, H.M. (2004) Prospects for transgenesis in the chick. Mech. Dev. 121: 1179–1186.
Ivarie, R. (2003) Avian transgenesis: progress towards the promise. Trends. Biotechnol. 21 : 14–19.
Mizuarai, S. Ono, K. Yamaguchi, K. Nishijima, K., Kamihira, M., and Iijima, S. (2001) Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector. Biochem. Biophys. Res. Commun. 286: 456–463.
Kamihira, M. Ono, K. Esaka, K. Nishijima, K., Kigaku, R., Komatsu, H., Yamashita, T., Kyogoku, K., and Iijima, S. (2005) High-level expression of scFv-Fc fusion protein in serum and egg white of genetically manipulated chickens using a retroviral vector. J. Virol. 79 : 10864–10874.
Kawabe, Y., Kamihira, M., Ono, K., Kyogoku, K., Nishijima, K., and Iijima, S. (2006) Production of scFv-Fc fusion protein using genetically manipulated quails. J. Biosci. Bioeng. 102 : 297–303.
Kodama, D., Nishimiya, D., Iwata, K., Yamaguchi, K., Yoshida, K., Kawabe, Y., Motono, M., Watanabe, H., Yamashita, T., Nishijima, K., Kamihira, M., and Iijima, S. (2008) Production of human erythropoietin by chimeric chickens. Biochem. Biophys. Res. Commun. 367 : 834–839.
Kyogoku, K., Yoshida, K., Watanabe, H., Yamashita, T., Kawabe, Y., Motono, M., Nishijima, K., Kamihira, M., and Iijima, S. (2008) Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens. J. Biosci. Bioeng. 105: 454–459.
Park, H.M., Haecker, S.E., Hagen, S.G., and Sanders, M.M. (2000) COUP-TF plays a dual role in the regulation of the ovalbumin gene. Biochemistry 39: 8537–8545.
Huber, M.C., Jägle, U., Krüqer, G., and Bonifer, C. (1997) The developmental activation of the chicken lysozyme locus in transgenic mice requires the interaction of a subset of enhancer elements with the promoter. Nucleic. Acids. Res. 25: 2992–3000.
Dierich, A., Gaub, M. P., LePennec, J.P, Astinotti, D., and Chambon, P. (1987) Cell-specificity of the chicken ovalbumin and conalbumin promoters. EMBO J. 6 : 2305–2312.
Gossen, M., and Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA. 89 : 5547–5551.
Sanders, M.M., and McKnight, G.S. (1985) Chicken egg white genes: multihormonal regulation in a primary cell culture system. Endocrinology 166 : 398–405.
Lillico, S.G., Sherman, A. McGrew, M.J., Robertson, C. D., Smith, J., Haslam, C., Barnard, P., Radcliffe, P.A., Mitrophanous, K.A., Elliot, E.A., and Sang, H.M. (2007) Oviduct-specific expression of two therapeutic proteins in transgenic hens. Proc. Natl. Acad. Sci. USA. 104(6): 1771–1776.
Zhu, L., van de Lavoir, M. C., Albanese, J., Beenhouwer, D. O., Cardarelli, P.M., Cuison, S. Deng, D.F., Deshpande, S., Diamond, J. H., Green, L., Halk, E.L., Heyer, B. S., Kay, R.M., Kerchner, A., Leighton, P.A., Mather, C.M., Morrison, S. L., Nikolov, Z.L., Passmore, D.B., Pradas-Monne, A., Preston, B.T., Rangan, V. S., Shi, M., Srinivasan, M., White, S. G., Winters-Digiacinto, P., Wong, S., Zhou, W., and Etches, R.J. (2005) Production of human monoclonal antibody in eggs of chimeric chickens. Nat. Biotechnol. 23(9): 1159–1169.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media B.V.
About this paper
Cite this paper
Kawabe, Y., Numata, K., Teramori, M., Ito, A., Kamihira, M. (2010). Development of Oviduct-Specific Gene Expression System for Transgenic Avian Bioreactor. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_33
Download citation
DOI: https://doi.org/10.1007/978-90-481-3892-0_33
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-3891-3
Online ISBN: 978-90-481-3892-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)