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Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

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Abstract

Monoclonal antibodies are widely used in molecular biology and in analytical diagnostic tests. The antibody market is increasing every year but to the issue remains as to how to improve the antibody production. In this study, we examined the possibility of solution after antibody purification for improvement in antibody production by CHO-DP12 cells. The method was effective for cell proliferation and antibody production. Furthermore, the proteins produced by the CHO-DP12 cells also improved the cell proliferation. These results suggest that the solution after antibody purification method may be an attractive resource of growth factors.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Suzuka National College of Technology, Suzuka, Mie, 510-0294, Japan

    Akiko Ogawa & Sadaharu Fukui

  2. Department of Applied Chemistry and Biotechnology, University of Fukui, Fukui, 910-8507, Japan

    Satoshi Terada

Authors
  1. Akiko Ogawa
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  2. Sadaharu Fukui
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  3. Satoshi Terada
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Corresponding author

Correspondence to Akiko Ogawa .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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© 2010 Springer Science+Business Media B.V.

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Ogawa, A., Fukui, S., Terada, S. (2010). Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_24

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