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FIS and Nucleoid Dynamics upon Exit from Lag Phase

  • Chapter
Bacterial Chromatin

Abstract

FIS is perhaps one of the most spectacular members of the small class of bacterial nucleoid-associated proteins. The transient pattern of FIS expression is unique and for no other protein of this class has been the spatial arrangement of multiple DNA binding sites so conspicuously related to function. The importance of the helical arrangement of FIS binding sites is especially obvious in synaptic complexes of DNA invertases and the transcription initiation complexes of stable RNA promoters. It is now apparent, that FIS can bind DNA cooperatively and that variable arrangements of DNA sites in conjunction with a wide range of binding site discrimination underlie the versatility and dynamics of the FIS nucleoprotein complexes. Furthermore, as an integral component of the homeostatic network regulating cellular DNA topology, FIS is so far the only highly abundant nucleoid-associated protein directly implicated in the control of genes coding for major cellular DNA topoisomerases. Nevertheless, the influence of FIS on global nucleoid architecture remains largely unknown. In this article we argue that modulation of DNA supercoil dynamics by FIS upon exit from lag phase facilitates conformational transitions of the nucleoprotein structures organized at two different - local and global - levels of complexity. At each level, the regulatory device involving FIS converts the analog information provided by supercoil dynamics of DNA into digital information uniquely encoded in the regulated gene(s). On this view, the mechanism of transcriptional regulation by topological transitions in the DNA molecule manifests a fractal character.

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Authors and Affiliations

  1. Jacobs University, Campus Ring 1, D-28759, Bremen, Germany

    Georgi Muskhelishvili

  2. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 OQH, UK

    Andrew Travers

  3. Fondation Pierre-Gilles de Gennes, c/o LBPA, Ecole Normale Superieure de Cachan, 9423, Cachan, France

    Andrew Travers

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  1. Georgi Muskhelishvili
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Correspondence to Georgi Muskhelishvili .

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  1. Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, Netherlands

    Remus T. Dame (Faculty of Mathematics and Natural Sciences) (Faculty of Mathematics and Natural Sciences)

  2. Division of Physics and Astronomy Section Physics of Complex Systems, VU University Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands

    Remus T. Dame (Faculty of Mathematics and Natural Sciences) (Faculty of Mathematics and Natural Sciences)

  3. Department of Microbiology School of Genetics and Microbiology, University of Dublin Trinity College, Dublin 2, Ireland

    Charles J. Dorman

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Muskhelishvili, G., Travers, A. (2010). FIS and Nucleoid Dynamics upon Exit from Lag Phase. In: Dame, R.T., Dorman, C.J. (eds) Bacterial Chromatin. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3473-1_14

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