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Purinergic Signalling in Pancreatic Islet Endothelial Cells

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Book cover Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function

Abstract

Both the microvascular endothelium and the endocrine cells in the pancreatic islet can release and react upon ATP. In support for the idea that intermittently released ATP, related to exocytosis of insulin secretory granules, affects the secretory activity of the capillary endothelium, we have demonstrated that isolated endothelial cells respond to activation of P2Y2 receptors with pronounced and extended rises of [Ca2+]i. The presence of such ATP effect is consistent with reports that β-cells regulate the blood flow within islets, where adenosine is a key mediator, and that the endothelial cell produce pro-angiogenic and angiostatic factors. In β-cells down-regulation of P2Y1 receptors results in disappearance of the transients of [Ca2+]i supposed to entrain these cells into a common rhythm. Since the islet endothelial cells respond to activation of P2Y2 receptors with extended elevation of [Ca2+]i, it is likely that the accompanying release of ATP is prolonged. Accordingly, the endothelial cells may have a tonic inhibitory action on the coordination of islet release pulses.

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Acknowledgments

Financial support for our own studies referred to in the article was received from the Swedish Research Council, the Swedish Diabetes Association, the Juvenile Diabetes Research Foundation and the Family Ernfors Fund.

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Authors and Affiliations

  1. Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Husargatan 3, Box 571, SE-751 23, Uppsala, Sweden

    Leif Jansson

  2. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden

    Eva Grapengiesser & Bo Hellman

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  1. Leif Jansson
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  2. Eva Grapengiesser
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Correspondence to Leif Jansson .

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Editors and Affiliations

  1. Dept. Pediatrics, Pediatric Clinical Services, University of Colorado, Denver, East 19th Avenue 12700, Aurora, 80045, USA

    Evgenia Gerasimovskaya

  2. Beth Israel Deaconess Medical Center, Harvard Medical School, Brookline Ave. 99, Boston, 02215, USA

    Elzbieta Kaczmarek

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Jansson, L., Grapengiesser, E., Hellman, B. (2010). Purinergic Signalling in Pancreatic Islet Endothelial Cells. In: Gerasimovskaya, E., Kaczmarek, E. (eds) Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3435-9_12

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