Skip to main content

Comparison of Cell Culture Methods for Obtaining of rHU-EPO to Large Scale

  • Conference paper
  • First Online:
Cells and Culture

Part of the book series: ESACT Proceedings ((ESACT,volume 4))

  • 1136 Accesses

Abstract

Continuous culture mode has not been used usually as a production choice for the manufacturing of biopharmaceuticals from animal cell culture. Instead, fed batch and perfusion are generally used. However for the production of unstable molecules, the continuous culture might represent a suitable process alternative.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Banik, G.G. and Heath, C.A. (1995) Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture. Biotechnol. Bioeng. 48, 289–300.

    Article  PubMed  CAS  Google Scholar 

  • Europa, A.F., Gambhir A., Fu, P.-C., Hu, W.-S. (1999) Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnol. Bioeng. 67(1), 25–34.

    Article  Google Scholar 

  • Yoon, S.K., Choi, S.L., Song, J.Y., Lee, G.M. (2004) Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0°C. Biotechnol. Bioeng. 89(3), 355–366.

    Google Scholar 

Download references

Acknowledgments

Authors want to thank to ESACT meeting organizing committee for bursary to José Arquímides Castro to attend to the 20th ESACT meeting.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to E. Ojito .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer Science+Business Media B.V.

About this paper

Cite this paper

Ojito, E. et al. (2010). Comparison of Cell Culture Methods for Obtaining of rHU-EPO to Large Scale. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_44

Download citation

Publish with us

Policies and ethics