Abstract
Exposure of transfected Chinese hamster ovary (CHO) cells to low temperature increased transient recombinant protein yield up to 18-fold. This phenomenon was not plasmid specific, but the enhancement of protein production under hypothermic conditions varied from 2- to 18-fold depending both on the reporter gene and the presence or absence of the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). When located in the 3′-untranslated region (UTR) of the transgene it increased protein production 3-fold at 31°C compared to expression from the isogenic plasmid without the element. Cold shock proteins are also known to act in a post-transcriptional manner, but the overexpression of the cold-induced RNA binding protein (CIRBP) and the RNA binding motif 3 (Rbm3) had no effect on the enhancement of transient recombinant protein production in CHO cells.
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Abbreviations
- CIRBP:
-
Cold Induced RNA Binding Protein
- Rbm3:
-
RNA Binding Motif 3
- WPRE:
-
Woodchuck hepatitis virus Post-transcriptional Regulatory Element
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Wulhfard, S., Tissot, S., Hacker, D.L., Wurm, F.M. (2010). Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_4
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DOI: https://doi.org/10.1007/978-90-481-3419-9_4
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