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Growth and Production Characteristics of Four Mammalian Cell Lines on a Cost-Effective Serum-Free Medium

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Cells and Culture

Part of the book series: ESACT Proceedings ((ESACT,volume 4))

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Abstract

In the current work we present results of using the simple cost-effective serum-free media for growth, transfection and protein expression in four mammalian producer cell lines in combination with simple and economical transfection procedure. SP2/0, 5E1/H3 hybridoma, CHO and EBNA 293 cell lines were adapted to suspension growth in DMEM/F12 based serum-free media. Biological activity of monoclonal antibodies produced by 5E1/H3 hybridomas was excellent for in vitro diagnostic use. Transient transfection procedure for suspension CHO cells with 25 kDa linear polyethylenimine (L-PEI) was optimised using expression of green fluorescent marker protein (EGFP). Using optimised transfection conditions the expression of therapeutically important recombinant protein CD44-3MUT in spinner flasks was maximal in cells at 48 h and in culture supernatant 72 h posttransfection.

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References

  • Hunt, R., Bragina, O., Drews, M., Kasak, L., Timmusk, S., Valkna, A., Kogerman, P., and Järvekülg, L. (2007) Generation and Characterization of mouse monoclonal antibody 5E1 against human transcription factor Gli3. Hybridoma 26(3), 131–138.

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  • Pall, T., Gad, A., Kasak, L., Drews, M., Stromblad, S., and Kogerman, P. (2004) Recombinant CD44-HABD is a novel and potent direct angiogenesis inhibitor enforcing endothelial cell-specific growth inhibition independently of hyaluronic acid binding. Oncogene 23(47), 7874–7881.

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Acknowledgment

This work was supported by EU Structural Fund and Estonian Governmental Fund.

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Authors and Affiliations

  1. Tallinn University of Technology and Competence Centre for Cancer Research, Akadeemia tee 15, 12618, Tallinn, Estonia

    M. Drews & R. Hunt

Authors
  1. M. Drews
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  2. R. Hunt
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Corresponding author

Correspondence to M. Drews .

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Editors and Affiliations

  1. Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

    Thomas Noll

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Drews, M., Hunt, R. (2010). Growth and Production Characteristics of Four Mammalian Cell Lines on a Cost-Effective Serum-Free Medium. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_38

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