Abstract
RNA-mediated gene silencing or RNA silencing is a gene regulation mechanism in eukaryotes involved in RNA-mediated sequence-specific RNA degradation in the cytoplasm followed by chromatin remodelling in the nucleus that plays a crucial role in differentiation and developmental processes. Besides, the RNA silencing machinery serves as an innate defense response against viruses and transposons and is responsible for down regulating transgene expression. Therefore, the induction of RNA silencing in producer cells limits the production of recombinant proteins and of (recombinant) virus particles for use as vaccines or in gene therapies.
Viruses counteract the RNA silencing response by expressing RNA silencing suppressors (RSSs) in infected cells, thereby allowing the virus to reach higher titers.
We employed viral RSSs for improving the productivity of eukaryotic production systems. We here describe the application of viral RSSs in enhancing the production of pharmaceutical proteins including monoclonal antibodies and virus particles in mammalian cell lines.
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de Haan, P., Engel, A., de Vries, W., van der Velden, J., van Holst, GJ., Arnoldussen, E. (2010). RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_2
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DOI: https://doi.org/10.1007/978-90-481-3419-9_2
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