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Regulation of Cell Death and Survival by RNA Interference – The Roles of miRNA and siRNA

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Apoptosome

Abstract

The elucidation of the intracellular mechanism for the processing of double-stranded RNAs was followed by a rapid implementation of RNAi technology as a standard tool in molecular biology and at the same time fostered the research into miRNA biogenesis and function.

This chapter reviews the mechanism of RNA interference and relevant criteria for the application of si/shRNA-mediated gene silencing as a research tool. Then, the role of endogenous miRNA for the regulation of apoptotic cell death in development and disease is illustrated on selected key pathologies. Finally, examples are given for the successful implementation of RNAi for the dissection of apoptotic cell death pathways and in experimental therapeutic approaches using apoptosis mediators as targets.

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Authors and Affiliations

  1. Department of Neurology, Georg-August-University Göttingen, University Medicine, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    Paul Lingor

  2. DFG Research Center for Molecular Physiology of the Brain (CMPB), Göttingen, Germany

    Paul Lingor

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  1. Paul Lingor
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Correspondence to Paul Lingor .

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Editors and Affiliations

  1. Dipto. Biologia, Università di Roma, Tor Vergata, Via della Ricerca Scientifica, Roma, 00133, Italy

    Francesco Cecconi

  2. IRCCS, Fondazione Santa Lucia, Via Fosso di Fiorano, Roma, 00143, Italy

    Marcello D'Amelio

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Lingor, P. (2010). Regulation of Cell Death and Survival by RNA Interference – The Roles of miRNA and siRNA. In: Cecconi, F., D'Amelio, M. (eds) Apoptosome. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3415-1_5

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