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Transgenesis and Genomics in Forage Crops

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Molecular Techniques in Crop Improvement

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Abstract

Forage grasses and legumes are important for animal production as well as soil conservation and environmental protection. Grass species also provide opportunities for turf and amenity. Further recently some of grasses showing high biomass have been given attention to renewable biomass energy crops. Many forage crops have polyploidy property and exhibit obligate outbreeding reproductive habits. Conventional breeding for forage crops is mainly based on synthetic varieties through polycrosses among superior selected genotypes. This system presents important challenges for the implementation of molecular breeding techniques such as transgenesis and genomics. Transgenesis and genomics approaches in forage crops have not been well performed due to many species and each species with relatively less importance compared to major cereals. However, recently transgenesis and genomics researches have been developed mainly for Lolium and Festuca species in grasses and Triflolium and Medicago species in legumes. In this charter current status of molecular breeding in forage crops is reviewed. Functionally-associated genetic markers which may be validated through association analysis will become important a tool for molecular breeding in near future. Author’s group has research activities on genetic improvement of cold temperature tolerance in forage grasses. Some of our results on candidate gene approach are also described.

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References

  • Alm V, Fang C, Busso CS, Devos KM, Vollan K, Grieg Z, Rognli OA (2003) A linkage map of meadow fescue (Festuca pratensis Huds.) and comparative mapping with other Poaceae species. Theor Appl Genet 108: 25–40

    PubMed  CAS  Google Scholar 

  • Altpeter F, Xu JP, Ahmed S (2000) Generation of large numbers of independently transformed fertile perennial ryegrass (Lolium perenne L.) plants of forage- and turf-type cultivars. Mol Breed 6:519–528

    CAS  Google Scholar 

  • Altpeter F, Fang YD, Xu JP, Ma XR (2004) Comparison of transgene expression stability after Agrobacterium-mediated or biolistic gene transfer into perennial ryegrass (Lolium perenne L.). In: Hopkins A, Wang ZY, Mian R, Sledge M, Barker RE (eds) Molecular breeding of forage and turf. Kluwer Academic, Dordrecht, pp 255–260

    Google Scholar 

  • Andersen JR, Lübberstedt T (2003) Functional markers in plants. Trends Plant Sci 8:554–560

    PubMed  CAS  Google Scholar 

  • Armstead IP, Turner LB, Farrell M, Skøt L, Gomez P, Montoya T, Donnison IS, King IP, Humphreys MO (2004) Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice. Theor Appl Genet 108:822–828

    PubMed  CAS  Google Scholar 

  • Asp T, Frei UK, Didion T, Nielsen KK, Lübberstedt T (2007) Frequency, type, and distribution of EST-SSRs from three genotypes of Lolium perenne, and their conservation across orthologous sequences of Festuca arundinacea, Brachypodium distachyon, and Oryza sativa. BMC Plant Biol 7:36

    PubMed  Google Scholar 

  • Auzanneau J, Huyghe C, Julier B, Barre P (2007) Linkage disequilibrium in synthetic varieties of perennial ryegrass. Theor Appl Genet 115:837–847

    PubMed  CAS  Google Scholar 

  • Bajaj S, Ran Y, Phillips J, Kularajathevan G, Pal S, Cohen D, Elborough K, Puthigae S (2006) A high throughput Agrobacterium tumefaciens-mediated transformation method for functional genomics of perennial ryegrass (Lolium perenne L.). Plant Cell Rep 25:651–659

    PubMed  CAS  Google Scholar 

  • Balance C-MC, McManus MT (2006) Expression of 1-aminocyclopropane-1-carboxylate (ACC) oxidase genes during the development of vegetative tissues in white clover (Trifolium repens L.) is regulated by ontological cues. Plant Mol Biol 60:451–467

    Google Scholar 

  • Barone P, Rosellini D, LaFayette P, Bouton J, Veronesi F, Parrot W (2008) Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa. Plant Cell Rep 27:893–901

    PubMed  CAS  Google Scholar 

  • Barrett B, Griffiths A, Schreiber M, Ellison N, Mercer C, Bouton J, Ong B, Forster J, Sawbridge T, Spangenberg G, Bryan G, Woodfield D (2004) A microsatellite map of white clover (Trifolium repens L.). Theor Appl Genet 109:596–608

    PubMed  CAS  Google Scholar 

  • Barrett BA, Baird IJ, Woodfield DR (2005) A QTL analysis of white clover seed production. Crop Sci 45:1844–1850

    CAS  Google Scholar 

  • Barrett BA, Baird IJ, Woodfield DR (2008) White clover seed yield: a case study in marker-assisted selection. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage crops. Springer Science + Business Media, New York, pp 241–250

    Google Scholar 

  • Bettany AJE, Dalton SJ, Timms E, Manderyck B, Dhanoa MS, Morris P (2003) Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.). Plant Cell Rep 21:437–444

    PubMed  CAS  Google Scholar 

  • Bouton JH (2008) Molecular breeding to improve forages for use in animal and biofuel production systems. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage crops. Springer Science + Business Media, New York, pp 1–13

    Google Scholar 

  • Brummer EC, Casler MD (2008) Improving selection in forage, turf, and biomass crops using molecular markers. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage crops. Springer Science + Business Media, New York, pp 193–209

    Google Scholar 

  • Cai H-W, Yuyama N, Tamaki H, Yoshizawa A (2003) Isolation and characterisation of simple sequence repeat markers in the hexaploid forage grass timothy (Phleum pratense L.). Theor Appl Genet 107:1337–1349

    PubMed  CAS  Google Scholar 

  • Cai H, Inoue M, Yuyama N, Nakayama S (2005a) An AFLP-based linkage map of Zoysiagrass (Zoysia japonica). Plant Breed 123:543–548

    Google Scholar 

  • Cai H, Inoue M, Yuyama N, Takahashi W, Hirata M, Sasaki T (2005b) Isolation, characterization and mapping of simple sequence repeat markers in zoysiagrass (Zoysia spp.). Theor Appl Genet 112:158–166

    PubMed  CAS  Google Scholar 

  • Cai H-W, Inoue M, Yuyama N, Hirata M (2008) Genome mapping in cool-season forage grasses. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage crops. Springer Science + Business Media, New York, pp 173–192

    Google Scholar 

  • Cervigni GDL, Paniego N, M Di’az, Selva JP, Zappacosta D, Zanazzi, Landerreche I, Martelotto L, Felitti S, Pessino S, Spangenberg G, Echenique V (2008) Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula. Plant Mol Biol 67:1–10

    PubMed  CAS  Google Scholar 

  • Chalmers J, Johnson X, Lidgett A, Spangenberg G (2003) Isolation and characterisation of a sucrose: sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne L.). J Plant Physiol 160:1385–1391

    PubMed  CAS  Google Scholar 

  • Chalmers J, Lidgett A, Cummings N, Cao Y, Forster J, Spangenberg G (2005) Molecular genetics of fructan metabolism in perennial ryegrass. Plant Biotechnol J 3:459–474

    PubMed  CAS  Google Scholar 

  • Chatterton NJ, Harrison PA, Bennett JH, Asay KH (1989) Carbohydrate partitioning in 185 accessions of Gramineae under warm and cool temperatures. J Plant Physiol 134:169–179

    CAS  Google Scholar 

  • Chen L, Auh C, Dowling P, Bell J, Chen F, Hopkins A, Dixon RA, Wang Z-Y (2003) Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down-regulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol J 1:437–449

    PubMed  CAS  Google Scholar 

  • Chen L, Auh C, Dowling P, Bell J, Lehmann D, Wang Z-Y (2004) Transgenic down-regulation of caffeic acid O-methyltransferase (COMT) led to improved digestibility in tall fescue (Festuca arundinacea). Funct Plant Biol 31:235–245

    CAS  Google Scholar 

  • Chen X, Yang WQ, Sivamani E, Bruneau AH, Wang BH, Qu RD (2005) Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E. coli argE gene. Mol Breed 15:339–347

    CAS  Google Scholar 

  • Cho MJ, Ha CD, Lemaux PG (2000) Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues. Plant Cell Rep 19:1084–1089

    CAS  Google Scholar 

  • Cho MJ, Choi HW, Lemaux PG (2001) Transformed T0 orchardgrass (Dactylis glomerata L.) plants produced from highly regenerative tissues derived from mature seeds. Plant Cell Rep 20:318–324

    CAS  Google Scholar 

  • Christou P (1992) Genetic transformation of crop plants using microprojectile bombardment. Plant J 2:275–281

    CAS  Google Scholar 

  • Cogan NOI, Smith KF, Yamada T, Francki MG, Vecchies AC, Jones ES, Spangenberg GC, Forster JW (2005) QTL analysis and comparative genomics of herbage quality traits in perennial ryegrass (Lolium perenne L.). Theor Appl Genet 110:364–380

    PubMed  CAS  Google Scholar 

  • Cogan NOI, Abberton MT, Smith KF, Kearney G, Marshall AH, Williams A, Michaelson-Yeates TPT, Bowen C, Jones ES, Vecchies AC, Forster JW (2006a) Individual and multi-environment combined analyses identify QTLs for morphogenetic and reproductive development traits in white clover (Trifolium repens L.). Theor Appl Genet 112:1401–1415

    PubMed  CAS  Google Scholar 

  • Cogan NOI, Ponting RC, Vecchies AC, Drayton MC, George J, Dobrowolski MP, Sawbridge TI, Spangenberg GC, Smith KF, Forster JW (2006b) Gene-associated single nucleotide polymorphism (SNP) discovery in perennial ryegrass (Lolium perenne L.). Mol Genet Genomics 276:101–112

    PubMed  CAS  Google Scholar 

  • Cogan NOI, Drayton MC, Ponting RC, Vecchies AC, Bannan NR, Sawbridge TI, Smith KF, Spangenberg GC, Forster JW (2007) Validation of in silico-predicted genic SNPs in white clover (Trifolium repens L.), an outbreeding alloplyploid species. Mol Genet Genomics 277:413–425

    PubMed  CAS  Google Scholar 

  • Crane C, Wright E, Dixon RA, Wang Z-Y (2006) Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens-transformed roots and Agrobacterium rhizogenes-transformed hairy roots. Planta 223:1344–1354

    PubMed  CAS  Google Scholar 

  • Curley J, Sim SC, Warnke S, Leong S, Barker R, Jung G (2005) QTL mapping of resistance to grey leaf spot in ryegrass. Theor Appl Genet 111:1107–1117

    PubMed  CAS  Google Scholar 

  • Dalton SJ, Bettany AJE, Timms E, Morris P (1999) Co-transformed, diploid Lolium perenne (Perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (Darnel) plants produced by microprojectile bombardment. Plant Cell Rep 18:721–726

    CAS  Google Scholar 

  • Denchev PD, Songstad DD, McDaniel JK, Conger BV (1997) Transgenic orchardgrass (Dactylis glomerata) plants by direct embryogenesis from microprojectile bombarded leaf cells. Plant Cell Rep 16:813–819

    CAS  Google Scholar 

  • Diwan N, Bouton JH, Kochert G, Cregan PB (2000) Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa. Theor Appl Genet 101:165–172

    CAS  Google Scholar 

  • Dobrowolski MP, Forster JW (2007) Linkage disequilibrium-based association mapping in forage species. In: Oraguzie NC, Rikkerink E, Gardiner SE, De Silva NH (eds) Association mapping in plants. Springer, New York, pp 197–209

    Google Scholar 

  • Dong S, Qu R (2005) High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Sci 168:1453–1458

    CAS  Google Scholar 

  • Dumsday JL, Smith KF, Forster JW, Jones ES (2003) SSR-based genetic linkage analysis of resistance to crown rust (Puccinia coronata Corda f. sp. lolii) in perennial ryegrass (Lolium perenne L.). Plant Pathol 52:628–637

    Google Scholar 

  • Ergon A, Fang C, Jorgensen O, Aamlid TS, Rognli OA (2006) Quantitative trait loci controlling vernalisation requirement, heading time and number of panicles in meadow fescue (Festuca pratensis Huds.). Theor Appl Genet 112:232–242

    PubMed  CAS  Google Scholar 

  • Gadegaard G, Didion T, Folling M, Storgaard M, Andersen CH, Nielsen KK (2008) Improved fructan accumulation in perennial ryegrass transformed with the onion fructosyltransferase genes 1-SST and 6G-FFT. J Plant Physiol 165:1214–1225

    PubMed  CAS  Google Scholar 

  • Gao C, Jiang L, Folling M, Han L, Nielsen KK (2006) Generation of large numbers of transgenic Kentucky bluegrass (Poa pratensis L.) plants following biolistic gene transfer. Plant Cell Rep 25:19–25

    PubMed  CAS  Google Scholar 

  • Ge Y, Norton T, Wang Z-Y (2006) Transgenic zoysiagrass (Zoysia japonica) plants obtainedby Agrobacterium-mediated transformation. Plant Cell Rep 25:792–798

    PubMed  CAS  Google Scholar 

  • Gill G P, Wilcox PL, Whittaker DJ, Winz RA, Bickerstaff P, Echt CE, Kent J, Humphreys MO, Elborough KM, Gardner RC (2006) A framework linkage map of perennial ryegrass based on SSR markers. Genome 49:354–364

    PubMed  CAS  Google Scholar 

  • Gondo T, Tsuruta S, Akashi R, Kawamura O, Hoffmann F (2005) Green herbicide-resistant plants by particle inflow gun-mediated gene transfer to diploid bahiagrass (Paspalum notatum). J Plant Physiol 162:1367–1375

    PubMed  CAS  Google Scholar 

  • Gondo T, Matsumoto J, Tsuruta S, Yoshida M, Kawakami A, Terami F, Ebina M, Yamada T, Akashi R (2009) Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana). J Plant Physiol 166:435–441

    PubMed  CAS  Google Scholar 

  • Guo Y-D, Hisano H, Shimamoto Y, Yamada T (2009) Transformation of androgenic-derived Festulolium plants (Lolium perenne L. x Festuca pratensis Huds.) by Agrobacterium tumefaciens. Plant Cell Tissue Organ Cult 96:219–227

    CAS  Google Scholar 

  • Fang C, Aamlid TS, Jorgensen O, Rognli OA (2004) Phenotypic and genotypic variation in seed production traits within a full-sib family of meadow fescue. Plant Breed 123:241–246

    Google Scholar 

  • Farrar K, Asp T, Lübberstedt T, Xu M, Thomas AM, Christiansen C, Humphreys MO, Donnison IS (2007) Construction of two Lolium perenne BAC libraries and identification of BACs containing candidate genes for disease resistance and forage quality. Mol Breed 19:15–23

    CAS  Google Scholar 

  • Faville M, Vecchies AC, Schreiber M, Drayton MC, Hughes LJ, Jones ES, Guthridge KM, Smith KF, Sawbridge T, Spangenberg GC, Bryan GT, Forster JW (2004) Functionally-associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.). Theor Appl Genet 110:12–32

    PubMed  CAS  Google Scholar 

  • Forster JW, Jones ES, Kölliker R, Drayton MC, Dumsday J, Dupal MP, Guthridge KM, Mahoney NL, van Zijll de Jong E, Smith KF (2001) Development and Implementation of Molecular Markers for Forage Crop Improvement. In: Spangenberg G (ed) Molecular breeding of forage crops. Kluwer Academic, Dordrecht, pp 101–133

    Google Scholar 

  • Forster JW, Jones ES, Batley J, Smith KF (2004) Molecular marker-based genetic analysis of pasture and turf grasses. In: Hopkins A, Wang Z-Y, Sledge M, Barker RE (eds) Molecular breeding of Forage and Turf. Kluwer Academic, Dordrecht, pp 197–239

    Google Scholar 

  • Francia E, Barabaschi D, Tondelli A, Laidò G, Rizza F, Stanca AM, Busconi M, Fogher C, Stockinger EJ, Pecchioni N (2007) Fine mapping of a Hv CBF gene cluster at the frost resistance locus Fr-H2 in barley. Theor Appl Genet 115:1083–1091

    PubMed  CAS  Google Scholar 

  • Fredslund J, Madsen LH, Hougaard BK, Nielsen AM, Bertioli D, Sandal N, Stougaard J, Schauser L (2006) A general pipeline for the development of anchor markers for comparative genomics in plants. BMC Genomics 7:207

    PubMed  Google Scholar 

  • Ha CD, Lemaux PG, Cho MJ (2001) Stable transformation of a recalcitrant Kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues. In Vitro Cell Dev Biol - Plant 37:6–11

    CAS  Google Scholar 

  • Hendry GAF (1993) Evolutionary origins and natural functions of fructans - a climatological, biogeographic and mechanistic appraisal. New Phytol 123:3–14

    CAS  Google Scholar 

  • Herrmann D, Boller B, Studer B, Widmer F, Kölliker R (2006) QTL analysis of seed yield components in red clover (Trifolium pratense L.). Theor Appl Genet 112:536–545

    PubMed  CAS  Google Scholar 

  • Herrmann D, Boller B, Studer B, Widmer F, Kölliker R (2008) Improving persistence in red clover: insights from QTL analysis and comparative phenotypic evaluation. Crop Sci 48:269–277

    Google Scholar 

  • Hirata M, Cai H, Inoue M, Yuyama N, Miura Y, Komatsu T, Takamizo T, Fujimori M (2006) Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 113:270–279

    PubMed  CAS  Google Scholar 

  • Hisano H, Kanazawa A, Kawakami A, Yoshida M, Shimamoto Y, Yamada T (2004) Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. Plant Sci 167:861–868

    CAS  Google Scholar 

  • Hisano H, Kanazawa A, Yoshida M, Humphreys MO, Iizuka M, Kitamura K, Yamada T (2008) Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass. New Phytol 178:766–780

    PubMed  CAS  Google Scholar 

  • Ikeda S, Takahashi W, Oishi H (2004) Generation of expressed sequence tags from cDNA libraries of Italian ryegrass (Lolium multiflorum Lam.). Grassl Sci 49:593–598

    Google Scholar 

  • Inoue M, Gao ZS, Hirata M, Fujimori M, Cai HW (2004a) Construction of a high-density map of Italian ryegrass (Lolium multiflorum Lam.) using restriction fragment length polymorphism, amplified fragment length polymorphism and telomeric repeat-associated sequence markers. Genome 47:57–65

    PubMed  CAS  Google Scholar 

  • Inoue M, Gao ZS, Cai HW (2004b) QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 109:1576–1585

    PubMed  CAS  Google Scholar 

  • International Rice Genome Sequencing Project (2005) The map-based sequence of the rice genome. Nature 436:793–800

    Google Scholar 

  • Ishikawa G, Yonemaru J, Saito M, Nakamura T (2007) PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes. BMC Genomics 8:135

    PubMed  Google Scholar 

  • Isobe S, Klimenko I, Ivashuta S, Gau M, Kozlov NN (2003) First RFLP linkage map of red clover (Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm. Theor Appl Genet 108:105–112

    PubMed  CAS  Google Scholar 

  • Isobe S, Nakaya A, Tabata S (2007) Genotype matrix mapping: searching for quantitative trait loci interactions in genetic variation in complex traits. DNA Res 14:217–225

    PubMed  CAS  Google Scholar 

  • Jenkins CLD, Snow AJ, Simpson RJ, Higgins TJ, Jacques NA, Pritchard J, Gibson J, Larkin PJ (2002) Fructan formation in transgenic white clover expressing a fructosyltransferase from Streptococcus salivarius. Funct Plant Biol 29:1287–1298

    CAS  Google Scholar 

  • Jensen LB, Andersen JR, Frei U, Xing Y, Taylor C, Holm PB, Lübberstedt T (2005) QTL mapping of vernalisation response in perennial ryegrass (Lolium perenne L.) reveals co-location with an orthologue of wheat VRN1. Theor Appl Genet 110:527–536

    PubMed  CAS  Google Scholar 

  • Jensen LB, Holm PB, Lübberstedt T (2007) Cross-species amplification of 105 Lolium perenne SSR loci in 23 species within the Poaceae. Mol Ecol Not 7:1155–1161

    CAS  Google Scholar 

  • Jones ES, Dupal MP, Kölliker R, Drayton MC, Forster JW (2001) Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.). Theor Appl Genet 102:405–415

    CAS  Google Scholar 

  • Jones ES, Mahoney NL, Hayward MD, Armstead IP, Jones JG, Humphreys MO, King IP, Kishida T, Yamada T, Balfourier F, Charmet C, Forster JW (2002a) An enhanced molecular marker-based map of perennial ryegrass (Lolium perenne L.) reveals comparative relationships with other Poaceae species. Genome 45:282–295

    PubMed  CAS  Google Scholar 

  • Jones ES, Dupal MD, Dumsday JL, Hughes LJ, Forster JW (2002b) An SSR-based genetic linkage map for perennial ryegrass (Lolium perenne L.). Theor Appl Genet 105:577–584

    PubMed  CAS  Google Scholar 

  • Jones ES, Hughes LJ, Drayton MC, Abberton MT, Michaelson-Yeates TPT, Forster JW (2003) An SSR and AFLP molecular marker-based genetic map of white clover (Trifolium repens L.). Plant Sci 165:531–539

    CAS  Google Scholar 

  • Kalla R, Chu P, Spangenberg G (2001) Moleular breeding of forage luguems for virus resistance. In: Spangenberg G (ed) Molecular breeding of forage and turf. Kluwer Academic, Dordrecht, pp 219–237

    Google Scholar 

  • Kawakami A, Yoshida M (2002) Molecular characterization of sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase associated with fructan accumulation in winter wheat during cold hardening. Biosci Biotechnol Biochem 66:2297–2305

    PubMed  CAS  Google Scholar 

  • Kölliker R, Jones ES, Drayton MC, Dupal MP, Forster JW (2001) Development and characterisation of simple sequence repeat (SSR) markers for white clover (Trifolium repens L.). Theor Appl Genet 102:416–424

    Google Scholar 

  • Kubik C, Sawkins M, Meyer WA, Gaut BS (2001) Genetic diversity in seven perennial ryegrass (Lolium perenne L.) cultivars based on SSR markers. Crop Sci 41:1565–1572

    CAS  Google Scholar 

  • Lasseur B, Lothier J, Djoumad A, De Coninck B, Smeekens S, Van Laere A, Morvan-Bertrand A, Van den Ende W, Prud’homme MP (2006) Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/ fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.). J Exp Bot 57:2719–2734

    PubMed  CAS  Google Scholar 

  • Lee S-H, Lee D-G, Woo H-S, Lee K-W, Kim D-H, Kwak S-S, Kim J-S, Kim H, Ahsan N, Choi MS, Yang J-K, Lee B-H (2006) Production of transgenic orchardgrass via Agrobacterium-mediated transformation of seed-derived callus tissues. Plant Sci 171:408–414

    CAS  Google Scholar 

  • Lem P, Lallemand J (2003) Grass consensus STS markers: an efficient approach for detecting polymorphism in Lolium. Theor Appl Genet 107:1113–1122

    PubMed  CAS  Google Scholar 

  • Li Q, Robson PRH, Bettany AJE, Donnison IS, Thomas H, Scott IM (2004) Modification of senescence in ryegrass transformed with IPT under the control of a monocot senescence-enhanced promoter. Plant Cell Rep 22:816–821

    PubMed  CAS  Google Scholar 

  • Mackie JM, Musial JM, Armour DJ, Phan HTT, Ellwood SE, Aitken KS, Irwin JAG (2007) Identification of QTL for reaction to three races of Colletotrichum trifolii and further analysis of inheritance of resistance in autotetraploid Lucerne. Theor Appl Genet 114:1417–1426

    PubMed  CAS  Google Scholar 

  • Miura Y, Ding CL, Ozaki R, Hirata M, Fujimori M, Takahashi W, Cai HW, Mizuno K (2005) Development of EST-derived CAPS and AFLP markers linked to a gene for resistance to ryegrass blast (Pyricularia sp.) in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 111:811–818

    PubMed  CAS  Google Scholar 

  • Montague A, Ziauddin A, Lee R, Ainley WM, Strommer J (2007) High-efficiency phosphinothricin-based selection for alfalfa transformation. Plant Cell Tissue Organ Cult 91:29–36

    CAS  Google Scholar 

  • Musial JM, Aitken KS, Mackie JM, Irwin JAG (2005) A genetic linkage map in autotetraploid lucerne adapted to northern Australia, and the use of the map to identify DNA markers linked to resistance to Phytophthora medicaginis. Aust J Agric Res 56:333–344

    CAS  Google Scholar 

  • Musial JM, Aitken KS, Mackie JM, Irwin JAG (2006) DNA markers linked to yield, yield components, and morphological traits in autotetraploid lucerne (Medicago sativa L.). Aust J Agric Res 57:801–810

    CAS  Google Scholar 

  • Musial JM, Mackie JM, Armour DJ, Phan HTT, Ellwood SE, Aitken KS, Irwin JAG (2007) Identification of QTL for resistance and susceptibility to Stagonospora meliloti in autotetraploid Lucerne. Theor Appl Genet 114:1427–1435

    PubMed  CAS  Google Scholar 

  • Muylle H, Baert J, Van Bockstaele E, Petijs J, Roldán-Ruiz I (2005a) Four QTLs determine crown rust (Puccinia coronata f.sp. lolii) resistance in a perennial ryegrass (Lolium perenne) population. Heredity 95:348–357

    PubMed  CAS  Google Scholar 

  • Muylle H, Baert J, Van Bockstaele E, Moerkerke B, Goetghebeur E, Roldán-Ruiz I (2005b) Identification of molecular markers linked with crown rust (Puccinia coronata f.sp. lolii) resistance in perennial ryegrass (Lolium perenne) using AFLP markers and a bulked segregant approach. Euphytica 143:135–144

    CAS  Google Scholar 

  • Nakashima K, Yamaguchi-Shinozaki K (2006) Regulons inovolved in osmotic stress-responsive and cold stress-responsive gene expression in plants. Physiol Plant 126:62–71

    CAS  Google Scholar 

  • Narasimhamoorthy B, Bouton JH, Olsen KM, Sledge MK (2007) Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa. Theor Appl Genet 114:901–913

    PubMed  CAS  Google Scholar 

  • Pavis N, Boucaud J, Prud’homme MP (2001) Fructans and fructan-metabolizing enzymes in leaves of Lolium perenne. New Phytol 150:97–109

    CAS  Google Scholar 

  • Petrovska N, Wu X, Donato R, Wang Z-Y, Ong E-K, Jones E, Forster J, Emmerling M, Sidoli A, O’Hehir R, Spangenberg G (2004) Transgenic ryegrasses (Lolium spp.) with down-regulation of main pollen allergens. Mol Breed 14:489–501

    CAS  Google Scholar 

  • Ponting RC, Drayton MC, Cogan NOI, Dobrowolski MP, Spangenberg GC, Smith KF, Forster JW (2007) SNP discovery, validation, haplotype structure and linkage disequilibrium in full-length herbage nutritive quality genes of perennial ryegrass (Lolium perenne L.). Mol Genet Genomics 278:585–597

    PubMed  CAS  Google Scholar 

  • Richards HA, Rudas VA, Sun H, McDaniel JK, Tomaszewski Z, Conger BV (2001) Genetic transformation and hybridization: construction of a GFP-BAR plasmid and its use for switchgrass transformation. Plant Cell Rep 20:48–54

    CAS  Google Scholar 

  • Robins JG, Luth D, Campbell TA, Bauchan GR, He C, Viands DR, Hansen JL, Brummer EC (2007a) Genetic mapping of biomass production in tetraploid alfalfa. Crop Sci 47:1–10

    CAS  Google Scholar 

  • Robins JG, Bauchan GR, Brummer EC (2007b) Genetic mapping forage yield, plant height, and regrowth at multiple harvests in tetraploid alfalfa (Medicago sativa L.). Crop Sci 47:11–18

    CAS  Google Scholar 

  • Rosellini R, Capomaccio S, Ferradini N, Sardaro MLS, Nicolia A, Veronesi F (2007) Non-antibiotic, efficient selection for alfalfa genetic engineering. Plant Cell Rep 26:1035–1044

    PubMed  CAS  Google Scholar 

  • Saha MC, Mian MAR, Eujayl I, Zwonitzer JC, Wang L, May GD (2004) Tall fescue EST-SSR markers with transferability across several grass species. Theor Appl Genet 109:783–791

    PubMed  Google Scholar 

  • Saha MC, Mian R, Zwonitzer JC, Chekhovskiy K, Hopkins AA (2005) An SSR- and AFLP-based genetic linkage map of tall fescue (Festuca arundinacea Schreb.). Theor Appl Genet 110:323–336

    Google Scholar 

  • Saha MC, Cooper JD, Mian MAR, Chekhovskiy K, May GD (2006) Tall fescue genomic SSR markers: development and transferability across multiple grass species. Theor Appl Genet 113:1449–1458

    PubMed  CAS  Google Scholar 

  • Sanford JC (1988) The biolistic process. Trends Biotechnol 6:299–302

    CAS  Google Scholar 

  • Sato H, Takamizo T (2006) Agrobacterium tumefaciens-mediated transformation of forage-type perennial ryegrass (Lolium perenne L.). Grassland Sci 52:95–98

    CAS  Google Scholar 

  • Sato S, Isobe S, Asamizu E, Ohmido N, Kataoka R, Nakamura Y, Kaneko T, Sakurai N, Okumura K, Klimenko I, Sasamoto S, Wada T, Watanabe A, Kohara M, Fujishiro T, Tabata S (2005) Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.). DNA Res 12:301–364

    Google Scholar 

  • Sawbridge T, Ong E-K, Binnion C, Emmerling M, McInnes R, Meath K, Nguyen N, Nunan K, O’Neill M, O’Toole F, Rhodes C, Simmonds J, Tian P, Wearne K, Webster T, Winkworth A, Spangenberg G (2003a) Generation and analysis of expressed sequence tags in perennial ryegrass (Lolium perenne L.). Plant Sci 165:1089–1100

    CAS  Google Scholar 

  • Sawbridge T, Ong E-K, Binnion C, Emmerling M, Meath K, Nunan K, O’Neill O, O’Toole F, Simmonds J, Wearne K, Winkworth A, Spangenberg G (2003b) Generation and analysis of expressed sequence tags in white clover (Trifolium repens L.). Plant Sci 165:1077–1089

    CAS  Google Scholar 

  • Shinozuka H, Hisano H, Ponting RC, Jones ES, Cogan NOI, Forster JW, Yamada T (2005) Molecular cloning and genetic mapping of perennial ryegrass protein kinase CK2â–¡-subunit genes. Theor Appl Genet 112:167–177

    PubMed  CAS  Google Scholar 

  • Shinozuka H, Hisano H, Yoneyama S, Shimamoto Y, Jones ES, Forster JW, Yamada T, Kanazawa A (2006) Cold-responsive gene expression, linkage mapping and protein localization in the nucleus of glycine-rich RNA-binding protein gene from perennial ryegrass suggest a role for cold adaptation. Mol Genet Genomics 275:399–408

    PubMed  CAS  Google Scholar 

  • Sim S, Chang T, Curley J, Warnke SE, Barker R, Jung G (2005) Chromosomal rearrangements differentiating the ryegrass genome from the Triticeae, oat and rice genomes using common heterologous RFLP probes. Theor Appl Genet 110:1011–1019

    PubMed  CAS  Google Scholar 

  • Sim S, Diesburg K, Casler M, Jung G (2007) Mapping and comparative analysis of QTL for crown rust resistance in an Italian x perennial ryegrass population. Phytopathology 97:767–776

    PubMed  CAS  Google Scholar 

  • Skinner JS, Zitzewitz von J, Szűcs P, Marquez-Cedillo L, Filichkin T, Amundsen K, Stockinger EJ, Thomashow MF, Chen THH, Hayes PM (2005) Structural, functional, and phylogenetic characterization of a large CBF gene family in barley. Plant Mol Biol 59:533–551

    PubMed  CAS  Google Scholar 

  • Skøt L, Humphreys J, Humphreys MO, Thorogood D, Gallagher J, Sanderson R, Armstead IP, Thomas ID (2007) Association of candidate genes with flowering time and water-soluble carbohydrate content in Lolium perenne L. Genetics 177:535–547

    PubMed  Google Scholar 

  • Sledge MK, Ray IM, Jiang G (2005) An expressed sequence tag SSR map of tetraploid alfalfa (Medicago sativa L.). Theor Appl Genet 111:980–992

    PubMed  CAS  Google Scholar 

  • Smith KF, Dobrowolski MP, Cogan NOI, Spangenberg GC, Forster JW (2008) Utilizing linkage disequilibrium and association mapping to implement candidate gene based markers in perennial ryegrass breeding. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage and turf. Springer Science + Business Media, New York, pp 335–340

    Google Scholar 

  • Somleva MN, Tomaszewski Z, Conger BV (2002) Agrobacterium-mediated genetic transformation of switchgrass. Crop Sci 42:2080–2087

    CAS  Google Scholar 

  • Somleva MN, Snell KD, Beaulieu JJ, Peoples OP, Garrison BR, Patterson NA (2008) Production of polyhydroxybutyrate in switchgrass, a value-added co-product in an important lignocellulosic biomass crop. Plant Biotechnol J 6:663–678

    PubMed  CAS  Google Scholar 

  • Spangenberg G, Wang Z-Y, Wu XL, Nagel J, Iglesias VA, Potrykus I (1995a) Transgenic tall fescue (Festuca arundinacea) and red fescue (F. rubra) plants from microprojectile bombardment of embryogenic suspension cells. J Plant Physiol 145:693–701

    CAS  Google Scholar 

  • Spangenberg G, Wang Z-Y, Wu XL, Nagel J, Potrykus I (1995b) Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells. Plant Sci 108:209–217

    CAS  Google Scholar 

  • Spangenberg G, Kalla R, Lidgett A, Sawbridge T, Ong EK, John U (2001) Breeding forage plants in the genome era. In: Spangenberg G (ed) Molecular breeding of forage crops. Kluwer Academic, Dordrecht, pp 1–39

    Google Scholar 

  • Spangenberg GS, Forster JW, Edwards D, John U, Mouradov A, Emmerling M, Batley J, Felitti S, Cogan NOI, Smith KF, Dobrowolski MP (2005) Future directions in the molecular breeding of forage and turf. In: Humphreys MO (ed) Molecular breeding for the genetic improvement of forage crops and turf. Wageningen Academic, The Netherlands, pp 83–97

    Google Scholar 

  • Studer B, Boller B, Herrmann D, Bauer E, Posselt UK, Widmer F, Kölliker R (2006) Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.) Theor Appl Genet 113:66–671

    Google Scholar 

  • Studer B, Boller B, Bauer E, Posselt UK, Widmer F, Kölliker R (2007) Consistent detection of QTLs for crown rust resistance in Italian ryegrass (Lolium multiforum Lam.) across environments and phenotyping methods. Theor Appl Genet 115:9–17

    PubMed  Google Scholar 

  • Studer B, Asp T, Frei U, Hentrup S, Meally H, Guillard A, Barth S, Muylle H, Rolda’n-Ruiz I, Barre P, Koning-Boucoiran C, Uenk-Stunnenberg G, Dolstra O, Skøt L, Skøt KP, Turner LB, Humphreys MO, Köllliker R, Roulund N, Nielsen KK, Lübberstedt T (2008) Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.) Mol Breed 21:533–548

    CAS  Google Scholar 

  • Takahashi W, Oishi H, Ebina M, Takamizo T, Komatsu T (2002) Production of transgenic Italian ryegrass (Lolium multiflorum Lam.) via microprojectile bomberdment of embryogenic calli. Plant Biotechnol 19:241–249

    CAS  Google Scholar 

  • Takahashi W, Fujimori M, Miura Y, Komatsu T., Nishizawa Y, Hibi T, Takamizo T (2005) Increased resistance to crown rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene. Plant Cell Rep 23:811–818

    PubMed  CAS  Google Scholar 

  • Tamura K, Yamada T (2007) A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species. Theor Appl Genet 114:273–283

    PubMed  CAS  Google Scholar 

  • Tamura T, Yonemaru J, Hisano H, Kanamori H, King J, King IP, Tase K, Sanada Y, Komatsu T, Yamada T (2009) Development of the intron-flanking EST markers for the Lolium-Festuca complex using rice genomic information. Theor Appl Genet 118:1549–1560

    PubMed  CAS  Google Scholar 

  • Thorogood D, Kaiser WJ, Jones JG, Armstead I (2002) Self-incompatibility in ryegrass 12: genotyping and mapping the S and Z loci of Lolium perenne L. Heredity 88:385–390

    PubMed  CAS  Google Scholar 

  • Tobias CM, Twigg P, Hayden DM, Vogel KP, Mitchell RM, Lazo GR, Chow EK, Sarath G (2005) Analysis of expressed sequence tags and the identification of associated short tandem repeats in switchgrass. Theor Appl Genet 111:956–964

    PubMed  Google Scholar 

  • Tobias CM, Hayden DM, Twigg P, Sarath G (2006) Genic microsatellite markers derived from EST sequencesof switchgrass (Panicum virgatum L.). Mol Ecol Not 6:185–187

    CAS  Google Scholar 

  • Turner LB, Cairns AJ, Armstead IP, Ashton J, Skøt K, Whittaker D, Humphreys MO (2006) Dissecting the regulation of fructan metabolism in perennial ryegrass (Lolium perenne) with quantitative trait locus mapping. New Phytol 169:45–58

    PubMed  CAS  Google Scholar 

  • Turner LB, Cairns AJ, Armstead IP, Thomas H, Humphreys MW, Humphreys MO (2008) Does fructan have a functional role in physiological traits? Investigation by quantitative trait locus mapping. New Phytol 179:765–775

    PubMed  CAS  Google Scholar 

  • Wang Z-Y, Ge Y (2005a) Agrobacterium-mediated high efficiency transformation of tall fescue (Festuca arundinacea Schreb.). J Plant Physiol 162:103–113

    PubMed  CAS  Google Scholar 

  • Wang Z-Y, Ge Y (2005b) Rapid and efficient production of transgenic bermudagrass and creeping bentgrass bypassing the callus formation phase. Funct Plant Biol 32:769–776

    CAS  Google Scholar 

  • Wang Z-Y, Yamada T (2008) Cool-season forage grasses. In: Kole C, Hall TC (eds) Compendium of transgenic crop plants: transgenic cereals and forage grasses. Wiley-Blackwell, Hoboken, NJ, pp 199–210

    Google Scholar 

  • Wang Z-Y, Ye XD, Nagel J, Potrykus I, Spangenberg G (2001) Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants. Plant Cell Rep 20:213–219

    CAS  Google Scholar 

  • Wang Z-Y, Bell J, Ge YX, Lehmann D (2003a) Inheritance of transgenes in transgenic tall fescue (Festuca arundinacea Schreb.). In Vitro Cell Dev Biol Plant 39:277–282

    CAS  Google Scholar 

  • Wang Z-Y, Scott M, Bell J, Hopkins A, Lehmann D (2003b) Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies. Theor Appl Genet 107:406–412

    PubMed  CAS  Google Scholar 

  • Wang Z-Y, Lawrence R, Hopkins A, Bell J, Scott M (2004) Pollen-mediated transgene flow in the wind-pollinated grass species tall fescue (Festuca arundinacea Schreb.). Mol Breed 14:47–60

    Google Scholar 

  • Wang Z-Y, Bell J, Cheng X, Ge Y, Ma X, Wright E, Xiao X, Zhang J, Bouton J (2008) Transgenesis in forage crops. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage and turf. Springer Science + Business Media, New York, pp 335–340

    Google Scholar 

  • Warnke SE, Barker RE, Jung G, Rouf Mian MA, Saha MC, Brilman LA, Dupal MD, Forster JW (2004) Genetic linkage mapping of an annual x perennial ryegrass population. Theor Appl Genet 109:294–304

    PubMed  CAS  Google Scholar 

  • Wright E, Dixon RA, Wang Z-Y (2006) Medicago truncatula transformation using cotyledon explants. In: Wang K (ed) Agrobacterium protocols, 2nd edn. Humana Press, Totowa, NJ, pp 129–135

    Google Scholar 

  • Wu YY, Chen QJ, Chen M, Chen J, Wang XC (2005) Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na+/H+ antiporter gene. Plant Sci 169:65–73

    CAS  Google Scholar 

  • Wu YY, Chen QJ, Cui, XH, Chen H, Chen J, Wang XC (2007) Efficient regeneration and Agrobacterium-mediated stable transformation of perennial ryegrass. Russ J Plant Physiol 54:524–529

    CAS  Google Scholar 

  • Xie D-Y, Sharma SB, Wright E, Wang Z-Y, Dixon RA (2006) Metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor. Plant J 45:895–907

    PubMed  CAS  Google Scholar 

  • Xiong Y, Fei S-Z, Brummer EC, Moore KJ, Barker RE, Jung G, Curley J, Warnke SE (2007a) QTL analyses of fiber components and crude protein in an annual x perennial ryegrass interspecific hybrid population. Mol Breed 18:327–340

    Google Scholar 

  • Xiong Y, Fei S-Z, Arora R, Brummer EC, Barker RE, Jung G, Warnke SE (2007b) Identification of quantitative trait loci controlling winter hardiness in an annual x perennial ryegrass interspecific hybrid population. Mol Breed 19:125–136

    Google Scholar 

  • Xu JP, Schubert J, Altpeter F (2001) Dissection of RNA-mediated ryegrass mosaic virus resistance in fertile transgenic perennial ryegrass (Lolium perenne L.). Plant J 26:265–274

    PubMed  CAS  Google Scholar 

  • Yamada T, Forster JW (2005) QTL analysis and trait dissection in ryegrasses (Lolium spp.). In: Humphreys MO (ed) Molecular breeding for the genetic improvement of forage crops and turf. Wageningen Academic, The Netherlands, pp 43–53

    Google Scholar 

  • Yamada T, Jones ES, Cogan NOI, Vecchies AC, Nomura T, Hisano H, Shimamoto Y, Smith KF, Forster JW (2004) QTL analysis of morphological, developmental and winter hardiness-associated traits in perennial ryegrass (Lolium perenne L.). Crop Sci 44:925–935

    CAS  Google Scholar 

  • Yamada T, Forster JW, Humphreys MW, Takamizo T (2005) Genetics and molecular breeding in Lolium/Festuca grass species complex. Grassl Sci 51:89–106

    CAS  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stressed. Annu Rev Plant Biol 57:781–803

    PubMed  CAS  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (2008) DREB regulons in abiotic-stress-responsive gene expression in plants. In: Yamada T, Spangenberg G (eds) Molecular breeding of forage crops. Springer Science + Business Media, New York, pp 15–27

    Google Scholar 

  • Ye X, Wang Z-Y, Wu X, Potrykus I, Spangenberg G (1997) Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Rep 16:379–384

    CAS  Google Scholar 

  • Ye XD, Wu XL, Zhao H, Frehner M, Nosberger J, Potrykus I, Spangenberg G (2001) Altered fructan accumulation in transgenic Lolium multiflorum plants expressing a Bacillus subtilis sacB gene. Plant Cell Rep 20:205–212

    CAS  Google Scholar 

  • Yoshida M, Abe J, Moriyama M, Kuwabara T (1998) Carbohydrate levels among wheat cultivars varying in freezing tolerance and snow mold resistance during autumn and winter. Physiol Plant 103:8–16

    CAS  Google Scholar 

  • Zhang J-Y, Broeckling CD, Blancaflor EB, Sledge MK, Sumner LW, Wang Z-Y (2005) Overexpression of WXPI, a putative Medicago truncatula AP2 domain–containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerence in transgenic alfalfa (Medicago sativa). Plant J 42:689–707

    Google Scholar 

  • Zhang H, Lomba P, Altpeter F (2007a) Improved turf quality of transgenic bahiagrass (Paspalum notatum Flugge) constitutively expressing the ATHB16 gene, a repressor of cell expansion. Mol Breed 20:415–423

    CAS  Google Scholar 

  • Zhang L, Wu D, Zhang L, Yang C (2007b) Agrobacterium-mediated transformation of Japanese lawngrass (Zoysia japonica Steud.) containing a synthetic cryIA(b) gene from Bacillus thuringiensis Plant Breed 126:428–432

    CAS  Google Scholar 

  • Zhang Y, Sledge MK, Bouton JH (2007c) Genome mapping of white clover (Trifolium repens L.) and comparative analysis within the Trifolieae using cross-species SSR markers. Theor Appl Genet 114:1367–1378

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan

    Toshihiko Yamada, Xun Wang & Yukiko Aoyagi

  2. National Agricultural Research Center for Hokkaido Region, Sapporo, Japan

    Ken-ichi Tamura

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  1. Toshihiko Yamada
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  2. Ken-ichi Tamura
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  3. Xun Wang
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  4. Yukiko Aoyagi
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Correspondence to Toshihiko Yamada .

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  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Institute (IRRI), Social Sciences Division, International Rice Research, Metro Manila, Philippines

    D.S. Brar

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Yamada, T., Tamura, Ki., Wang, X., Aoyagi, Y. (2010). Transgenesis and Genomics in Forage Crops. In: Jain, S., Brar, D. (eds) Molecular Techniques in Crop Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2967-6_31

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