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Techniques in Plant Proteomics

  • Ludovít Škultéty
  • Maxym Danchenko
  • Anna Pret’ová
  • Martin Hajduch
Chapter

Abstract

Plant proteomics is a relatively new research field that focuses on large-scale functional analysis of plant proteins. This new research field has already demonstrated immense potential for getting significantly deeper insight into the functional interaction of plant proteins and their roles in plant growth and development. The knowledge of key proteins responsible for valuable crop traits in the context of expression of other involved proteins plays pivotal role in discovery phase of crop improvement effort. This chapter introduces the field of plant proteomics in practical manner. Set of six figures helps to understand principles of protein extraction, separation and identification by mass spectrometry. Protein and peptide separation is introduced in greater details taking into account “gel-based” and “gel-free” methods.

Keywords

Sodium Dodecyl Sulphate Protein Identification Peptide Mass Fingerprinting Poppy Seed Column Packing Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The work was supported by FP7 of the European Union (MIRG-CT-2007-200165). This chapter reflects only the author’s views and the Community is not liable for any use that might be made of information contained herein.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ludovít Škultéty
    • 1
  • Maxym Danchenko
    • 2
  • Anna Pret’ová
    • 2
  • Martin Hajduch
    • 2
  1. 1.Institute of Virology, Centre of Molecular Medicine, Slovak Academy of SciencesBratislavaSlovakia
  2. 2.Institute of Plant Genetics and Biotechnology, Slovak Academy of SciencesNitraSlovakia

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