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Aspergillosis: From Diagnosis to Prevention pp 105–124Cite as

Galactomannan Testing

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Abstract

Inhaled conidia (spores) of Aspergillus spp. are usually effectively cleared by phagocytic cells, such as alveolar macrophages, neutrophils and monocytes, without clinical consequence. However, a depressed immune system presents risks for invasive aspergillosis (IA), mainly affecting the lungs, and, to a lesser extent, the sinuses. At particular risk for invasive disease are allogeneic haematopoietic stem cell transplant (HSCT) recipients –, and patients who have received intensive myelotoxic therapy for haematological malignancies, resulting in deep and prolonged neutropenia .

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References

  1. Segal, B. H. (2007) Role of macrophages in host defense against aspergillosis and strategies for immune augmentation. Oncologist, 12(Suppl 2), 7–13.

    CAS  PubMed  Google Scholar 

  2. Maschmeyer, G., Haas, A. & Cornely, O. A. (2007) Invasive aspergillosis: epidemiology, diagnosis and management in immunocompromised patients. Drugs, 67, 1567–601.

    Article  CAS  PubMed  Google Scholar 

  3. Marr, K. A., Carter, R. A., Crippa, F., Wald, A. & Corey, L. (2002) Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients. Clin Infect Dis, 34, 909–17.

    Article  PubMed  Google Scholar 

  4. Pagano, L., Caira, M., Nosari, A., Van Lint, M. T., Candoni, A., Offidani, M., Aloisi, T., Irrera, G., Bonini, A., Picardi, M., Caramatti, C., Invernizzi, R., Mattei, D., Melillo, L., De Waure, C., Reddiconto, G., Fianchi, L., Valentini, C. G., Girmenia, C., Leone, G. & Aversa, F. (2007) Fungal infections in recipients of hematopoietic stem cell transplants: results of the SEIFEM B-2004 study–Sorveglianza Epidemiologica Infezioni Fungine Nelle Emopatie Maligne. Clin Infect Dis, 45, 1161–70.

    Article  CAS  PubMed  Google Scholar 

  5. Martino, R., Subira, M., Rovira, M., Solano, C., Vazquez, L., Sanz, G. F., Urbano-Ispizua, A., Brunet, S. & De La Camara, R. (2002) Invasive fungal infections after allogeneic peripheral blood stem cell transplantation: incidence and risk factors in 395 patients. Br J Haematol, 116, 475–82.

    Article  PubMed  Google Scholar 

  6. Pagano, L., Caira, M., Candoni, A., Offidani, M., Fianchi, L., Martino, B., Pastore, D., Picardi, M., Bonini, A., Chierichini, A., Fanci, R., Caramatti, C., Invernizzi, R., Mattei, D., Mitra, M. E., Melillo, L., Aversa, F., Van Lint, M. T., Falcucci, P., Valentini, C. G., Girmenia, C. & Nosari, A. (2006) The epidemiology of fungal infections in patients with hematologic malignancies: the SEIFEM-2004 study. Haematologica, 91, 1068–75.

    PubMed  Google Scholar 

  7. Hope, W. W., Walsh, T. J. & Denning, D. W. (2005) Laboratory diagnosis of invasive aspergillosis. Lancet Infect Dis, 5, 609–22.

    Article  CAS  PubMed  Google Scholar 

  8. Hachem, R., Sumoza, D., Hanna, H., Girgawy, E., Munsell, M. & Raad, I. (2006) Clinical and radiologic predictors of invasive pulmonary aspergillosis in Cancer patients: should the European Organization for Research and Treatment of Cancer/Mycosis Study Group (EORTC/MSG) criteria be revised? Cancer, 106, 1581–6.

    Article  PubMed  Google Scholar 

  9. Greene, R. E., Schlamm, H. T., Oestmann, J. W., Stark, P., Durand, C., Lortholary, O., Wingard, J. R., Herbrecht, R., Ribaud, P., Patterson, T. F., Troke, P. F., Denning, D. W., Bennett, J. E., De Pauw, B. E. & Rubin, R. H. (2007) Imaging findings in acute invasive pulmonary aspergillosis: clinical significance of the halo sign. Clin Infect Dis, 44, 373–9.

    Article  PubMed  Google Scholar 

  10. Caillot, D., Couaillier, J. F., Bernard, A., Casasnovas, O., Denning, D. W., Mannone, L., Lopez, J., Couillault, G., Piard, F., Vagner, O. & Guy, H. (2001) Increasing volume and changing characteristics of invasive pulmonary aspergillosis on sequential thoracic computed tomography scans in patients with neutropenia. J Clin Oncol, 19, 253–9.

    CAS  PubMed  Google Scholar 

  11. Kojima, R., Tateishi, U., Kami, M., Murashige, N., Nannya, Y., Kusumi, E., Sakai, M., Tanaka, Y., Kanda, Y., Mori, S., Chiba, S., Kusumoto, M., Miyakoshi, S., Hirai, H., Taniguchi, S., Sakamaki, H. & Takaue, Y. (2005) Chest computed tomography of late invasive aspergillosis after allogeneic hematopoietic stem cell transplantation. Biol Blood Marrow Transplant, 11, 506–11.

    Article  PubMed  Google Scholar 

  12. Walsh, T. J., Anaissie, E. J., Denning, D. W., Herbrecht, R., Kontoyiannis, D. P., Marr, K. A., Morrison, V. A., Segal, B. H., Steinbach, W. J., Stevens, D. A., Van Burik, J. A., Wingard, J. R. & Patterson, T. F. (2008) Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis, 46, 327–60.

    Article  CAS  PubMed  Google Scholar 

  13. Kontoyiannis, D. P., Lionakis, M. S., Lewis, R. E., Chamilos, G., Healy, M., Perego, C., Safdar, A., Kantarjian, H., Champlin, R., Walsh, T. J. & RAAD, I. I. (2005) Zygomycosis in a tertiary-care cancer center in the era of Aspergillus-active antifungal therapy: a case-control observational study of 27 recent cases. J Infect Dis, 191, 1350–60.

    Article  PubMed  Google Scholar 

  14. Perfect, J. R., Cox, G. M., Lee, J. Y., Kauffman, C. A., De Repentigny, L., Chapman, S. W., Morrison, V. A., Pappas, P., Hiemenz, J. W. & Stevens, D. A. (2001) The impact of culture isolation of Aspergillus species: a hospital-based survey of aspergillosis. Clin Infect Dis, 33, 1824–33.

    Article  CAS  PubMed  Google Scholar 

  15. Ascioglu, S., Rex, J. H., De Pauw, B., Bennett, J. E., Bille, J., Crokaert, F., Denning, D. W., Donnelly, J. P., Edwards, J. E., Erjavec, Z., Fiere, D., Lortholary, O., Maertens, J., Meis, J. F., Patterson, T. F., Ritter, J., Selleslag, D., Shah, P. M., Stevens, D. A. & Walsh, T. J. (2002) Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis, 34, 7–14.

    Article  CAS  PubMed  Google Scholar 

  16. Young, R. C. & Bennett, J. E. (1971) Invasive aspergillosis. Absence of detectable antibody response. Am Rev Respir Dis, 104, 710–6.

    CAS  PubMed  Google Scholar 

  17. Bardana, E. J., Jr., Gerber, J. D., Craig, S. & Cianciulli, F. D. (1975) The general and specific humoral immune response to pulmonary aspergillosis. Am Rev Respir Dis, 112, 799–805.

    PubMed  Google Scholar 

  18. Tomee, J. F. & Kauffman, H. F. (2000) Putative virulence factors of Aspergillus fumigatus. Clin Exp Allergy, 30, 476–84.

    Article  CAS  PubMed  Google Scholar 

  19. Lehmann, P. F. & Reiss, E. (1978) Invasive aspergillosis: antiserum for circulating antigen produced after immunization with serum from infected rabbits. Infect Immun, 20, 570–2.

    CAS  PubMed  Google Scholar 

  20. Bennett, J. E., Bhattacharjee, A. K. & Glaudemans, C. P. (1985) Galactofuranosyl groups are immunodominant in Aspergillus fumigatus galactomannan. Mol Immunol, 22, 251–4.

    Article  CAS  PubMed  Google Scholar 

  21. Latge, J. P., Kobayashi, H., Debeaupuis, J. P., Diaquin, M., Sarfati, J., Wieruszeski, J. M., Parra, E., Bouchara, J. P. & Fournet, B. (1994) Chemical and immunological characterization of the extracellular galactomannan of Aspergillus fumigatus. Infect Immun, 62, 5424–33.

    CAS  PubMed  Google Scholar 

  22. Morelle, W., Bernard, M., Debeaupuis, J. P., Buitrago, M., Tabouret, M. & Latge, J. P. (2005) Galactomannoproteins of Aspergillus fumigatus. Eukaryot Cell, 4, 1308–16.

    Article  CAS  PubMed  Google Scholar 

  23. Mennink-Kersten, M. A., Donnelly, J. P. & Verweij, P. E. (2004) Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis. Lancet Infect Dis, 4, 349–57.

    Article  CAS  PubMed  Google Scholar 

  24. Sabetta, J. R., Miniter, P. & Andriole, V. T. (1985) The diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen. J Infect Dis, 152, 946–53.

    CAS  PubMed  Google Scholar 

  25. De Repentigny, L., Boushira, M., Ste-Marie, L. & Bosisio, G. (1987) Detection of galactomannan antigenemia by enzyme immunoassay in experimental invasive aspergillosis. J Clin Microbiol, 25, 863–7.

    PubMed  Google Scholar 

  26. Wilson, E. V., Hearn, V. M. & Mackenzie, D. W. (1987) Evaluation of a test to detect circulating Aspergillus fumigatus antigen in a survey of immunocompromised patients with proven or suspected invasive disease. J Med Vet Mycol, 25, 365–75.

    Article  CAS  PubMed  Google Scholar 

  27. Stynen, D., Sarfati, J., Goris, A., Prevost, M. C., Lesourd, M., Kamphuis, H., Darras, V. & Latge, J. P. (1992) Rat monoclonal antibodies against Aspergillus galactomannan. Infect Immun, 60, 2237–45.

    CAS  PubMed  Google Scholar 

  28. Swanink, C. M., Meis, J. F., Rijs, A. J., Donnelly, J. P. & Verweij, P. E. (1997) Specificity of a sandwich enzyme-linked immunosorbent assay for detecting Aspergillus galactomannan. J Clin Microbiol, 35, 257–60.

    CAS  PubMed  Google Scholar 

  29. Van Cutsem, J., Meulemans, L., Van Gerven, F. & Stynen, D. (1990) Detection of circulating galactomannan by Pastorex Aspergillus in experimental invasive aspergillosis. Mycoses, 33, 61–9.

    PubMed  Google Scholar 

  30. Dupont, B., Improvisi, L. & Provost, F. (1990) Détection de galactomannane dans les aspergilloses invasives humaines et animales avec un test au latex. Bull Soc Fr Mycol Med, 19, 35–41.

    Google Scholar 

  31. Rogers, T. R., Haynes, K. A. & Barnes, R. A. (1990) Value of antigen detection in predicting invasive pulmonary aspergillosis. Lancet, 336, 1210–3.

    Article  CAS  PubMed  Google Scholar 

  32. Haynes, K. & Rogers, T. R. (1994) Retrospective evaluation of a latex agglutination test for diagnosis of invasive aspergillosis in immunocompromised patients. Eur J Clin Microbiol Infect Dis, 13, 670–4.

    Article  CAS  PubMed  Google Scholar 

  33. Verweij, P. E., Stynen, D., Rijs, A. J., De Pauw, B. E., Hoogkamp-Korstanje, J. A. & Meis, J. F. (1995) Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients. J Clin Microbiol, 33, 1912–4.

    CAS  PubMed  Google Scholar 

  34. Sulahian, A., Tabouret, M., Ribaud, P., Sarfati, J., Gluckman, E., Latge, J. P. & Derouin, F. (1996) Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis. Eur J Clin Microbiol Infect Dis, 15, 139–45.

    Article  CAS  PubMed  Google Scholar 

  35. Kappe, R., Schulze-Berge, A. & Sonntag, H. G. (1996) Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis. Mycoses, 39, 13–23.

    Article  CAS  PubMed  Google Scholar 

  36. Machetti, M., Feasi, M., Mordini, N., Van Lint, M. T., Bacigalupo, A., Latge, J. P., Sarfati, J. & Viscoli, C. (1998) Comparison of an enzyme immunoassay and a latex agglutination system for the diagnosis of invasive aspergillosis in Bone Marrow Transplant recipients. Bone Marrow Transplant, 21, 917–21.

    Article  CAS  PubMed  Google Scholar 

  37. Herrmann, J., Gugel, A., Freidank, H., Bertz, H. & Finke, J. (1998) Aspergillus antigen detection: comparison of a new sandwich ELISA with the latex agglutination test in patients with histologically proven invasive aspergillosis. Mycoses, 41(Suppl 1), 83–5.

    Article  CAS  PubMed  Google Scholar 

  38. Kappe, R. & Schulze-Berge, A. (1993) New cause for false-positive results with the Pastorex Aspergillus antigen latex agglutination test. J Clin Microbiol, 31, 2489–90.

    CAS  PubMed  Google Scholar 

  39. Maertens, J., Theunissen, K., Lodewyck, T., Lagrou, K. & Van Eldere, J. (2007) Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders. Mycoses, 50(Suppl 1), 2–17.

    Article  CAS  PubMed  Google Scholar 

  40. Stynen, D., Goris, A., Sarfati, J. & Latge, J. P. (1995) A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis. J Clin Microbiol, 33, 497–500.

    CAS  PubMed  Google Scholar 

  41. Wheat, L. J. (2003) Rapid diagnosis of invasive aspergillosis by antigen detection. Transpl Infect Dis, 5, 158–66.

    Article  CAS  PubMed  Google Scholar 

  42. De Pauw, B., Walsh, T. J., Donnelly, J. P., Stevens, D. A., Edwards, J. E., Calandra, T., Pappas, P. G., Maertens, J., Lortholary, O., Kauffman, C. A., Denning, D. W., Patterson, T. F., Maschmeyer, G., Bille, J., Dismukes, W. E., Herbrecht, R., Hope, W. W., Kibbler, C. C., Kullberg, B. J., Marr, K. A., Munoz, P., Odds, F. C., Perfect, J. R., Restrepo, A., Ruhnke, M., Segal, B. H., Sobel, J. D., Sorrell, T. C., Viscoli, C., Wingard, J. R., Zaoutis, T. & Bennett, J. E. (2008) Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis, 46, 1813–21.

    Article  PubMed  Google Scholar 

  43. Upton, A., Gugel, A., Leisenring, W., Limaye, A., Alexander, B., Hayden, R. & Marr, K. A. (2005) Reproducibility of low galactomannan enzyme immunoassay index values tested in multiple laboratories. J Clin Microbiol, 43, 4796–800.

    Article  CAS  PubMed  Google Scholar 

  44. Verweij, P. E., Erjavec, Z., Sluiters, W., Goessens, W., Rozenberg-Arska, M., Debets-Ossenkopp, Y. J., Guiot, H. F. & Meis, J. F. (1998) Detection of antigen in sera of patients with invasive aspergillosis: intra- and interlaboratory reproducibility. The Dutch Interuniversity Working Party for Invasive Mycoses. J Clin Microbiol, 36, 1612–6.

    CAS  PubMed  Google Scholar 

  45. Marr, K. A., Balajee, S. A., Mclaughlin, L., Tabouret, M., Bentsen, C. & Walsh, T. J. (2004) Detection of galactomannan antigenemia by enzyme immunoassay for the diagnosis of invasive aspergillosis: variables that affect performance. J Infect Dis, 190, 641–9.

    Article  CAS  PubMed  Google Scholar 

  46. Sheppard, D. C., Marr, K. A., Fredricks, D. N., Chiang, L. Y., Doedt, T. & Filler, S. G. (2006) Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis. Clin Microbiol Infect, 12, 376–80.

    Article  CAS  PubMed  Google Scholar 

  47. Klont, R. R., Mennink-Kersten, M. A. & Verweij, P. E. (2004) Utility of Aspergillus antigen detection in specimens other than serum specimens. Clin Infect Dis, 39, 1467–74.

    Article  PubMed  Google Scholar 

  48. Meersseman, W., Lagrou, K., Maertens, J., Wilmer, A., Hermans, G., Vanderschueren, S., Spriet, I., Verbeken, E. & Van Wijngaerden, E. (2008) Galactomannan in bronchoalveolar lavage fluid: a tool for diagnosing aspergillosis in intensive care unit patients. Am J Respir Crit Care Med, 177, 27–34.

    Article  PubMed  Google Scholar 

  49. Husain, S., Clancy, C. J., Nguyen, M. H., Swartzentruber, S., Leather, H., Lemonte, A. M., Durkin, M. M., Knox, K. S., Hage, C. A., Bentsen, C., Singh, N., Wingard, J. R. & Wheat, L. J. (2008) Performance characteristics of the platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid. Clin Vaccine Immunol, 15, 1760–3.

    Article  CAS  PubMed  Google Scholar 

  50. Husain, S., Kwak, E. J., Obman, A., Wagener, M. M., Kusne, S., Stout, J. E., Mccurry, K. R. & Singh, N. (2004) Prospective assessment of Platelia Aspergillus galactomannan antigen for the diagnosis of invasive aspergillosis in lung transplant recipients. Am J Transplant, 4, 796–802.

    Article  PubMed  Google Scholar 

  51. Husain, S., Paterson, D. L., Studer, S. M., Crespo, M., Pilewski, J., Durkin, M., Wheat, J. L., Johnson, B., Mclaughlin, L., Bentsen, C., Mccurry, K. R. & Singh, N. (2007) Aspergillus galactomannan antigen in the bronchoalveolar lavage fluid for the diagnosis of invasive aspergillosis in lung transplant recipients. Transplantation, 83, 1330–6.

    Article  CAS  PubMed  Google Scholar 

  52. Boutboul, F., Alberti, C., Leblanc, T., Sulahian, A., Gluckman, E., Derouin, F. & Ribaud, P. (2002) Invasive aspergillosis in allogeneic stem cell transplant recipients: increasing antigenemia is associated with progressive disease. Clin Infect Dis, 34, 939–43.

    Article  PubMed  Google Scholar 

  53. Maertens, J., Glasmacher, A., Selleslag, D., Ngai, A., Ryan, D., Layton, M., Taylor, A., Sable, C. & Kartsonis, N. (2005) Evaluation of serum sandwich enzyme-linked immunosorbent assay for circulating galactomannan during caspofungin therapy: results from the caspofungin invasive aspergillosis study. Clin Infect Dis, 41, e9–14.

    Article  PubMed  Google Scholar 

  54. Woods, G., Miceli, M. H., Grazziutti, M. L., Zhao, W., Barlogie, B. & Anaissie, E. (2007) Serum Aspergillus galactomannan antigen values strongly correlate with outcome of invasive aspergillosis: a study of 56 patients with hematologic Cancer. Cancer, 110, 830–4.

    Article  PubMed  Google Scholar 

  55. Miceli, M. H., Grazziutti, M. L., Woods, G., Zhao, W., Kocoglu, M. H., Barlogie, B. & Anaissie, E. (2008) Strong correlation between serum aspergillus galactomannan index and outcome of aspergillosis in patients with hematological cancer: clinical and research implications. Clin Infect Dis, 46, 1412–22.

    Article  PubMed  Google Scholar 

  56. Pfeiffer, C. D., Fine, J. P. & Safdar, N. (2006) Diagnosis of invasive aspergillosis using a galactomannan assay: a meta-analysis. Clin Infect Dis, 42, 1417–27.

    Article  CAS  PubMed  Google Scholar 

  57. Herbrecht, R., Letscher-Bru, V., Oprea, C., Lioure, B., Waller, J., Campos, F., Villard, O., Liu, K. L., Natarajan-Ame, S., Lutz, P., Dufour, P., Bergerat, J. P. & Candolfi, E. (2002) Aspergillus galactomannan detection in the diagnosis of invasive aspergillosis in cancer patients. J Clin Oncol, 20, 1898–906.

    Article  PubMed  Google Scholar 

  58. Fortun, J., Martin-Davila, P., Alvarez, M. E., Sanchez-Sousa, A., Quereda, C., Navas, E., Barcena, R., Vicente, E., Candelas, A., Honrubia, A., Nuno, J., Pintado, V. & Moreno, S. (2001) Aspergillus antigenemia sandwich-enzyme immunoassay test as a serodiagnostic method for invasive aspergillosis in liver transplant recipients. Transplantation, 71, 145–9.

    Article  CAS  PubMed  Google Scholar 

  59. Kwak, E. J., Husain, S., Obman, A., Meinke, L., Stout, J., Kusne, S., Wagener, M. M. & Singh, N. (2004) Efficacy of galactomannan antigen in the Platelia Aspergillus enzyme immunoassay for diagnosis of invasive aspergillosis in liver transplant recipients. J Clin Microbiol, 42, 435–8.

    Article  PubMed  Google Scholar 

  60. Stephens-Romero, S. D., Mednick, A. J. & Feldmesser, M. (2005) The pathogenesis of fatal outcome in murine pulmonary aspergillosis depends on the neutrophil depletion strategy. Infect Immun, 73, 114–25.

    Article  CAS  PubMed  Google Scholar 

  61. Balloy, V., Huerre, M., Latge, J. P. & Chignard, M. (2005) Differences in patterns of infection and inflammation for corticosteroid treatment and chemotherapy in experimental invasive pulmonary aspergillosis. Infect Immun, 73, 494–503.

    Article  CAS  PubMed  Google Scholar 

  62. Verweij, P. E., Weemaes, C. M., Curfs, J. H., Bretagne, S. & Meis, J. F. (2000) Failure to detect circulating Aspergillus markers in a patient with chronic granulomatous disease and invasive aspergillosis. J Clin Microbiol, 38, 3900–1.

    CAS  PubMed  Google Scholar 

  63. Aquino, V. R., Goldani, L. Z. & Pasqualotto, A. C. (2007) Update on the contribution of galactomannan for the diagnosis of invasive aspergillosis. Mycopathologia, 163, 191–202.

    Article  CAS  PubMed  Google Scholar 

  64. Maertens, J., Verhaegen, J., Demuynck, H., Brock, P., Verhoef, G., Vandenberghe, P., Van Eldere, J., Verbist, L. & Boogaerts, M. (1999) Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive Aspergillosis. J Clin Microbiol, 37, 3223–8.

    CAS  PubMed  Google Scholar 

  65. Maertens, J., Verhaegen, J., Lagrou, K., Van Eldere, J. & Boogaerts, M. (2001) Screening for circulating galactomannan as a noninvasive diagnostic tool for invasive aspergillosis in prolonged neutropenic patients and stem cell transplantation recipients: a prospective validation. Blood, 97, 1604–10.

    Article  CAS  PubMed  Google Scholar 

  66. Maertens, J., Theunissen, K., Verbeken, E., Lagrou, K., Verhaegen, J., Boogaerts, M. & Eldere, J. V. (2004) Prospective clinical evaluation of lower cut-offs for galactomannan detection in adult neutropenic cancer patients and haematological stem cell transplant recipients. Br J Haematol, 126, 852–60.

    Article  CAS  PubMed  Google Scholar 

  67. Maertens, J. A., Klont, R., Masson, C., Theunissen, K., Meersseman, W., Lagrou, K., Heinen, C., Crepin, B., Van Eldere, J., Tabouret, M., Donnelly, J. P. & Verweij, P. E. (2007) Optimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay. Clin Infect Dis, 44, 1329–36.

    Article  CAS  PubMed  Google Scholar 

  68. Hope, W. W., Kruhlak, M. J., Lyman, C. A., Petraitiene, R., Petraitis, V., Francesconi, A., Kasai, M., Mickiene, D., Sein, T., Peter, J., Kelaher, A. M., Hughes, J. E., Cotton, M. P., Cotten, C. J., Bacher, J., Tripathi, S., Bermudez, L., Maugel, T. K., Zerfas, P. M., Wingard, J. R., Drusano, G. L. & Walsh, T. J. (2007) Pathogenesis of Aspergillus fumigatus and the kinetics of galactomannan in an in vitro model of early invasive pulmonary aspergillosis: implications for antifungal therapy. J Infect Dis, 195, 455–66.

    Article  CAS  PubMed  Google Scholar 

  69. Penack, O., Rempf, P., Graf, B., Blau, I. W. & Thiel, E. (2008) Aspergillus galactomannan testing in patients with long-term neutropenia: implications for clinical management. Ann Oncol, 19, 984–9.

    Article  CAS  PubMed  Google Scholar 

  70. Nguyen, M. H., Jaber, R., Leather, H. L., Wingard, J. R., Staley, B., Wheat, L. J., Cline, C. L., Baz, M., Rand, K. H. & Clancy, C. J. (2007) Use of bronchoalveolar lavage to detect galactomannan for diagnosis of pulmonary aspergillosis among nonimmunocompromised hosts. J Clin Microbiol, 45, 2787–92.

    Article  PubMed  Google Scholar 

  71. Clancy, C. J., Jaber, R. A., Leather, H. L., Wingard, J. R., Staley, B., Wheat, L. J., Cline, C. L., Rand, K. H., Schain, D., Baz, M. & Nguyen, M. H. (2007) Bronchoalveolar lavage galactomannan in diagnosis of invasive pulmonary aspergillosis among solid-organ transplant recipients. J Clin Microbiol, 45, 1759–65.

    Article  CAS  PubMed  Google Scholar 

  72. Siemann, M., Koch-Dorfler, M. & Gaude, M. (1998) False-positive results in premature infants with the Platelia Aspergillus sandwich enzyme-linked immunosorbent assay. Mycoses, 41, 373–7.

    Article  CAS  PubMed  Google Scholar 

  73. Maertens, J., Van Eldere, J., Verhaegen, J., Verbeken, E., Verschakelen, J. & Boogaerts, M. (2002) Use of circulating galactomannan screening for early diagnosis of invasive aspergillosis in allogeneic stem cell transplant recipients. J Infect Dis, 186, 1297–306.

    Article  CAS  PubMed  Google Scholar 

  74. Mennink-Kersten, M. A., Klont, R. R., Warris, A., Op Den Camp, H. J. & Verweij, P. E. (2004) Bifidobacterium lipoteichoic acid and false ELISA reactivity in aspergillus antigen detection. Lancet, 363, 325–7.

    Article  CAS  PubMed  Google Scholar 

  75. Mennink-Kersten, M. A., Ruegebrink, D., Klont, R. R., Warris, A., Gavini, F., Op Den Camp, H. J. & Verweij, P. E. (2005) Bifidobacterial lipoglycan as a new cause for false-positive platelia Aspergillus enzyme-linked immunosorbent assay reactivity. J Clin Microbiol, 43, 3925–31.

    Article  CAS  PubMed  Google Scholar 

  76. Sulahian, A., Touratier, S. & Ribaud, P. (2003) False positive test for aspergillus antigenemia related to concomitant administration of piperacillin and tazobactam. N Engl J Med, 349, 2366–7.

    Article  CAS  PubMed  Google Scholar 

  77. Viscoli, C., Machetti, M., Cappellano, P., Bucci, B., Bruzzi, P., Van Lint, M. T. & Bacigalupo, A. (2004) False-positive galactomannan platelia Aspergillus test results for patients receiving piperacillin-tazobactam. Clin Infect Dis, 38, 913–6.

    Article  PubMed  Google Scholar 

  78. Adam, O., Auperin, A., Wilquin, F., Bourhis, J. H., Gachot, B. & Chachaty, E. (2004) Treatment with piperacillin-tazobactam and false-positive Aspergillus galactomannan antigen test results for patients with hematological malignancies. Clin Infect Dis, 38, 917–20.

    Article  PubMed  Google Scholar 

  79. Singh, N., Obman, A., Husain, S., Aspinall, S., Mietzner, S. & Stout, J. E. (2004) Reactivity of platelia Aspergillus galactomannan antigen with piperacillin-tazobactam: clinical implications based on achievable concentrations in serum. Antimicrob Agents Chemother, 48, 1989–92.

    Article  CAS  PubMed  Google Scholar 

  80. Walsh, T. J., Shoham, S., Petraitiene, R., Sein, T., Schaufele, R., Kelaher, A., Murray, H., Mya-San, C., Bacher, J. & Petraitis, V. (2004) Detection of galactomannan antigenemia in patients receiving piperacillin-tazobactam and correlations between in vitro, in vivo, and clinical properties of the drug-antigen interaction. J Clin Microbiol, 42, 4744–8.

    Article  CAS  PubMed  Google Scholar 

  81. Penack, O., Schwartz, S., Thiel, E. & Wolfgang Blau, I. (2004) Lack of evidence that false-positive Aspergillus galactomannan antigen test results are due to treatment with piperacillin-tazobactam. Clin Infect Dis, 39, 1401–2; author reply 2–3.

    Article  PubMed  Google Scholar 

  82. Wu, D. H. (2004) Platelia Aspergillus assay and potential cross-reaction. Clin Infect Dis, 39, 1402; author reply.

    Article  PubMed  Google Scholar 

  83. Mattei, D., Rapezzi, D., Mordini, N., Cuda, F., Lo Nigro, C., Musso, M., Arnelli, A., Cagnassi, S. & Gallamini, A. (2004) False-positive Aspergillus galactomannan enzyme-linked immunosorbent assay results in vivo during amoxicillin-clavulanic acid treatment. J Clin Microbiol, 42, 5362–3.

    Article  CAS  PubMed  Google Scholar 

  84. Maertens, J., Theunissen, K., Verhoef, G. & Van Eldere, J. (2004) False-positive Aspergillus galactomannan antigen test results. Clin Infect Dis, 39, 289–90.

    Article  PubMed  Google Scholar 

  85. Xavier, M. O., Pasqualotto, A. C., Cardoso, I. C. & Severo, L. C. (2009) Cross-reactivity of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Cryptococcus species in the commercial kit Platelia Aspergillus EIA. Clin Vaccine Immunol, 16, 132–3.

    Google Scholar 

  86. Cummings, J. R., Jamison, G. R., Boudreaux, J. W., Howles, M. J., Walsh, T. J. & Hayden, R. T. (2007) Cross-reactivity of non-Aspergillus fungal species in the Aspergillus galactomannan enzyme immunoassay. Diagn Microbiol Infect Dis, 59, 113–5.

    Article  CAS  PubMed  Google Scholar 

  87. Wheat, L. J., Hackett, E., Durkin, M., Connolly, P., Petraitiene, R., Walsh, T. J., Knox, K. & Hage, C. (2007) Histoplasmosis-associated cross-reactivity in the BioRad Platelia Aspergillus enzyme immunoassay. Clin Vaccine Immunol, 14, 638–40.

    Article  CAS  PubMed  Google Scholar 

  88. Hamaki, T., Kami, M., Kanda, Y., Miyakoshi, S., Ueyama, J., Morinaga, S. & Mutou, Y. (2001) False-positive results of Aspergillus enzyme-linked immunosorbent assay in a patient with chronic graft-versus-host disease after allogeneic Bone Marrow Transplantation. Bone Marrow Transplant, 28, 633–4.

    Article  CAS  PubMed  Google Scholar 

  89. Racil, Z., Kocmanova, I., Lengerova, M., Winterova, J. & Mayer, J. (2007) Intravenous PLASMA-LYTE as a major cause of false-positive results of platelia Aspergillus test for galactomannan detection in serum. J Clin Microbiol, 45, 3141–2.

    Article  PubMed  Google Scholar 

  90. Sheppard, D., Chabot, J., Kirkpatrick, W. R. et al. (2007) Effect of antifungal agents on galactomannan kinetics in vivo. in 47th Interscience Conference on Antimicrobial Agents and Chemotherapy. Chicago, USA.

    Google Scholar 

  91. Martino, R. & Viscoli, C. (2006) Empirical antifungal therapy in patients with neutropenia and persistent or recurrent fever of unknown origin. Br J Haematol, 132, 138–54.

    Article  CAS  PubMed  Google Scholar 

  92. Marr, K. A., Laverdiere, M., Gugel, A. & Leisenring, W. (2005) Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin Infect Dis, 40, 1762–9.

    Article  CAS  PubMed  Google Scholar 

  93. Mennink-Kersten, M. A., Ruegebrink, D., Klont, R. R., Warris, A., Blijlevens, N. M., Donnelly, J. P. & Verweij, P. E. (2008) Improved detection of circulating Aspergillus antigen by use of a modified pretreatment procedure. J Clin Microbiol, 46, 1391–7.

    Article  PubMed  Google Scholar 

  94. Sanguinetti, M., Posteraro, B., Pagano, L., Pagliari, G., Fianchi, L., Mele, L., La Sorda, M., Franco, A. & Fadda, G. (2003) Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis. J Clin Microbiol, 41, 3922–5.

    Article  CAS  PubMed  Google Scholar 

  95. Becker, M. J., Lugtenburg, E. J., Cornelissen, J. J., Van Der Schee, C., Hoogsteden, H. C. & De Marie, S. (2003) Galactomannan detection in computerized tomography-based broncho-alveolar lavage fluid and serum in haematological patients at risk for invasive pulmonary aspergillosis. Br J Haematol, 121, 448–57.

    Article  CAS  PubMed  Google Scholar 

  96. Musher, B., Fredricks, D., Leisenring, W., Balajee, S. A., Smith, C. & Marr, K. A. (2004) Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. J Clin Microbiol, 42, 5517–22.

    Article  CAS  PubMed  Google Scholar 

  97. Platelia [Package insert]. Marnes-La-Coquette, Bio-Rad.

    Google Scholar 

  98. Rohrlich, P., Sarfati, J., Mariani, P., Duval, M., Carol, A., Saint-Martin, C., Bingen, E., Latge, J. P. & Vilmer, E. (1996) Prospective sandwich enzyme-linked immunosorbent assay for serum galactomannan: early predictive value and clinical use in invasive aspergillosis. Pediatr Infect Dis J, 15, 232–7.

    Article  CAS  PubMed  Google Scholar 

  99. Bretagne, S., Marmorat-Khuong, A., Kuentz, M., Latge, J. P., Bart-Delabesse, E. & Cordonnier, C. (1997) Serum Aspergillus galactomannan antigen testing by sandwich ELISA: practical use in neutropenic patients. J Infect, 35, 7–15.

    Article  CAS  PubMed  Google Scholar 

  100. Sulahian, A., Boutboul, F., Ribaud, P., Leblanc, T., Lacroix, C. & Derouin, F. (2001) Value of antigen detection using an enzyme immunoassay in the diagnosis and prediction of invasive aspergillosis in two adult and pediatric hematology units during a 4-year prospective study. Cancer, 91, 311–8.

    Article  CAS  PubMed  Google Scholar 

  101. Ulusakarya, A., Chachaty, E., Vantelon, J. M., Youssef, A., Tancrede, C., Pico, J. L., Bourhis, J. H., Fenaux, P. & Munck, J. N. (2000) Surveillance of Aspergillus galactomannan antigenemia for invasive aspergillosis by enzyme-linked immunosorbent assay in neutropenic patients treated for hematological malignancies. Hematol J, 1, 111–6.

    Article  CAS  PubMed  Google Scholar 

  102. Pinel, C., Fricker-Hidalgo, H., Lebeau, B., Garban, F., Hamidfar, R., Ambroise-Thomas, P. & Grillot, R. (2003) Detection of circulating Aspergillus fumigatus galactomannan: value and limits of the Platelia test for diagnosing invasive aspergillosis. J Clin Microbiol, 41, 2184–6.

    Article  CAS  PubMed  Google Scholar 

  103. Rovira, M., Jimenez, M., De La Bellacasa, J. P., Mensa, J., Rafel, M., Ortega, M., Almela, M., Martinez, C., Fernandez-Aviles, F., Martinez, J. A., Urbano-Ispizua, A., Carreras, E. & Montserrat, E. (2004) Detection of Aspergillus galactomannan by enzyme immunoabsorbent assay in recipients of allogeneic hematopoietic stem cell Transplantation: a prospective study. Transplantation, 77, 1260–4.

    Article  CAS  PubMed  Google Scholar 

  104. Kawazu, M., Kanda, Y., Nannya, Y., Aoki, K., Kurokawa, M., Chiba, S., Motokura, T., Hirai, H. & Ogawa, S. (2004) Prospective comparison of the diagnostic potential of real-time PCR, double-sandwich enzyme-linked immunosorbent assay for galactomannan, and a (1-->3)-beta-D-glucan test in weekly screening for invasive aspergillosis in patients with hematological disorders. J Clin Microbiol, 42, 2733–41.

    Article  CAS  PubMed  Google Scholar 

  105. Buchheidt, D., Hummel, M., Schleiermacher, D., Spiess, B., Schwerdtfeger, R., Cornely, O. A., Wilhelm, S., Reuter, S., Kern, W., Sudhoff, T., Morz, H. & Hehlmann, R. (2004) Prospective clinical evaluation of a LightCycler-mediated polymerase chain reaction assay, a nested-PCR assay and a galactomannan enzyme-linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients. Br J Haematol, 125, 196–202.

    Article  CAS  PubMed  Google Scholar 

  106. Pazos, C., Ponton, J. & Del Palacio, A. (2005) Contribution of (1->3)-beta-D-glucan chromogenic assay to diagnosis and therapeutic monitoring of invasive aspergillosis in neutropenic adult patients: a comparison with serial screening for circulating galactomannan. J Clin Microbiol, 43, 299–305.

    Article  CAS  PubMed  Google Scholar 

  107. Yoo, J. H., Choi, J. H., Choi, S. M., Lee, D. G., Shin, W. S., Min, W. S. & Kim, C. C. (2005) Application of nucleic acid sequence-based amplification for diagnosis of and monitoring the clinical course of invasive aspergillosis in patients with hematologic diseases. Clin Infect Dis, 40, 392–8.

    Article  CAS  PubMed  Google Scholar 

  108. Busca, A., Locatelli, F., Barbui, A., Limerutti, G., Serra, R., Libertucci, D. & Falda, M. (2006) Usefulness of sequential Aspergillus galactomannan antigen detection combined with early radiologic evaluation for diagnosis of invasive pulmonary aspergillosis in patients undergoing allogeneic stem cell transplantation. Transplant Proc, 38, 1610–3.

    Article  CAS  PubMed  Google Scholar 

  109. Suankratay, C., Kanitcharaskul, P. & Arunyingmongkol, K. (2006) Galactomannan antigenemia for the diagnosis of invasive aspergillosis in neutropenic patients with hematological disorders. J Med Assoc Thai, 89, 1851–8.

    PubMed  Google Scholar 

  110. Steinbach, W. J., Addison, R. M., Mclaughlin, L., Gerrald, Q., Martin, P. L., Driscoll, T., Bentsen, C., Perfect, J. R. & Alexander, B. D. (2007) Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients. Pediatr Infect Dis J, 26, 558–64.

    Article  PubMed  Google Scholar 

  111. Lai, C. C., Hsu, H. L., Lee, L. N. & Hsueh, P. R. (2007) Assessment of Platelia Aspergillus enzyme immunoassay for the diagnosis of invasive aspergillosis. J Microbiol Immunol Infect, 40, 148–53.

    PubMed  Google Scholar 

  112. Yoo, J. H., Choi, S. M., Lee, D. G., Park, S. H., Choi, J. H., Kwon, E. Y. & Shin, W. S. (2007) Comparison of the real-time nucleic acid sequence-based amplification (RTi-NASBA) with conventional NASBA, and galactomannan assay for the diagnosis of invasive aspergillosis. J Korean Med Sci, 22, 672–6.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Haematology, University Hospitals Leuven, Leuven, Belgium

    Johan Maertens

  2. Department of Haematology, Virga Jesse Hospital, Hasselt, Belgium

    Koen Theunissen

  3. Department of Medical Diagnostic Sciences, University Hospitals Leuven, Leuven, Belgium

    Katrien Lagrou

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  1. Johan Maertens
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  2. Koen Theunissen
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  3. Katrien Lagrou
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Correspondence to Johan Maertens .

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  1. Porto Alegre, Complexo Hospitalar Santa Casa de, Av. Independencia 155, Porto Alegre, 90035-075, Brazil

    Alessandro Comarú Pasqualotto

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Maertens, J., Theunissen, K., Lagrou, K. (2009). Galactomannan Testing. In: Comarú Pasqualotto, A. (eds) Aspergillosis: From Diagnosis to Prevention. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2408-4_7

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