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Neural Progenitors

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Book cover Human Adult Stem Cells

Part of the book series: Human Cell Culture ((HUCC,volume 7))

Abstract

Several derivation techniques have been published [1–11] describing both free floating aggregate and adherent human NSC/NPC cultures under a variety of growth factor regimes [3, 12–45]. We describe here, a detailed reproducible methodology for the successful isolation, expansion, and preservation of bona fide human fetal (10–24 weeks) NPC that relies on a specific temporal combination of mitogenic growth factors (EGF, bFGF, and LIF) and is independent of whether cells are cultured adherent or as aggregates. We have implemented strict selection and expansion criteria to further exclude more restricted cellular phenotypes from the stem/progenitor pool during the initial derivation procedure. Selection criteria ensure that cells fulfill both an operational definition of a stem cell as well as retain engraftability in multiple experimental models. For simplicity sake, we will refrain from the stem vs. progenitor debate [46-51] and simply refer to both neural stem and progenitor cells as NPCs from here forward.

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Authors and Affiliations

  1. The Burnham Institute for Medical Research, University of California at San Diego, Graduate Program in Biomedical Sciences, 9500 Gilman Dr., La Jolla, CA 92093, USA

    Dustin R. Wakeman

  2. The Burnham Institute for Medical Research, La Jolla, CA 92037, USA

    Martin R. Hofmann

  3. Departments of Neurosurgery, and Physical Medicine and Rehabilitation, Harvard Medical School, Brigham and Women’s Hospital and Spaulding Rehabilitation Hospital; Division of Spinal Cord Injury Research, Veterans Affairs Boston Healthcare System, Boston, MA 02130, USA

    Yang D. Teng

  4. Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA

    Evan Y. Snyder

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  1. Dustin R. Wakeman
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  2. Martin R. Hofmann
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  3. Yang D. Teng
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  4. Evan Y. Snyder
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Correspondence to Evan Y. Snyder .

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Editors and Affiliations

  1. University College London Inst. Urology & Nephrology, 67 Riding House Street, London, United Kingdom W1P 7PN

    John R. Masters

  2. Dept. Bioengineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0412, USA

    Bernhard Ø. Palsson

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Wakeman, D.R., Hofmann, M.R., Teng, Y.D., Snyder, E.Y. (2009). Neural Progenitors. In: Masters, J.R., Palsson, B.Ø. (eds) Human Adult Stem Cells. Human Cell Culture, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2269-1_1

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