Unbiased clustering methods

  • Alessandro Cellerino
  • Michele Sanguanini
Part of the CRM Series book series (PSNS, volume 17)


After DEG analysis, the RNA-seq dataset becomes a matrix of vertically-organised vectors, where the rows correspond to the genes (with identifiers associated with gene annotation databases) and the columns to attributes such as fold-change (e.g., up-regulated, downregulated, non-differentially regulated genes), p-value, FDR corrected p-value, or other relevant information and measures (Table 5.1). At this point, the numerosity of the data could easily overwhelm the human user and it is not unusual for a ‘wet-lab’ scientist to struggle and fail to extract relevant information from and make biological sense out of long lists of DEGs.


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Copyright information

© Scuola Normale Superiore Pisa 2018

Authors and Affiliations

  • Alessandro Cellerino
    • 1
  • Michele Sanguanini
    • 2
  1. 1.Scuola Normale SuperiorePisaItaly
  2. 2.Gonville and Caius CollegeUniversity of CambridgeCambridge, CambridgeshireUnited Kingdom

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