Next-generation RNA sequencing
The Next-generation RNA sequencing (RNA-seq) is an high-throughput experimental technique (and design) that allows sequencing of cDNA at very high redundancy (depth), in the order of 107 individual sequences (reads) per sample. This technique can provide a quantification-by-sequencing of RNA abundance and analysis of RNA sequence variation from biological samples. The development of high-throughput technologies applied to biology has opened exciting avenues for computational and data-driven biology1.
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