Chlamydia pneumoniae Infections: Applications of Laboratory Methods

  • R. W. Peeling


Since the recognition of Chlamydia pneumoniae as a species distinct from C. psittaci in 1989, laboratory methods have gradually been developed as tools for the management of C. pneumoniae infection, for defining the epidemiology of disease and for research into the pathogenesis of chlamydial infections [1, 2]. These laboratory methods include culture, antigen detection, serology and the polymerase chain reaction (PCR). Early seroepidemiological studies were based on the micro-immunofluorescence (MIF) assay, originally developed for C. trachomatis. As the conditions for culture of C. pneumoniae become optimised, antigenic and genetic differences of strains from around the world were documented. Antigen detection and PCR assays represent a new generation of tests which can provide rapid diagnosis with ease of specimen transport. The attributes and limitations of each of these laboratory methods will be presented below, followed by a discussion on how these tests can be applied appropriately in different settings.


Chlamydial Infection Antigen Detection Complement fIxation Test Direct Fluorescent Antibody Chlamydia Species 
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© Springer-Verlag Italia, Milano 1999

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  • R. W. Peeling

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