In Vitro Experimental Methods for Assessing Tracheobronchial Smooth Muscle Contraction

  • P. C. Braga


Both the physiological and pathological changes in the mechanical behavior of tracheobronchial smooth muscles (shortening or development of tension) have been widely studied. There have been many studies to characterize and to reproduce the muscular aspects of the “asthma phenomenon” and pharmacological correction of it.


Airway Smooth Muscle Smooth Muscle Contraction Organ Bath Bronchial Smooth Muscle Isotonic Contraction 
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  1. 1.
    Magnus R (1904) Versuche am überlebenden Dünndarm von Säugetieren. Pflugers Arch Physiol 102: 123Google Scholar
  2. 2.
    Hawkins DF, Schild HO (1951) The action of drugs on isolated human bronchial chains. Br J Pharmacol 6: 682–690Google Scholar
  3. 3.
    Department of Pharmacology, University of Edinburgh (1968) Pharmacological experiments on isolated preparations. Livingstone, EdinburghGoogle Scholar
  4. 4.
    Castillo JC,de Beer EJ (1947) A tracheal chain. 1. A preparation for the study of antispasmodics with particular reference to bronchodilator drugs. J Pharmacol 90: 104–109Google Scholar
  5. 5.
    Akcasu A (1952) The actions of drugs on the isolated trachea. J Pharm Pharmacol 4: 671–676PubMedGoogle Scholar
  6. 6.
    Akcasu A (1959) The physiologic and pharmacologic characteristics of the tracheal muscle. Arch Int Pharmacodyn Ther 122: 201–207PubMedGoogle Scholar
  7. 7.
    Foster WR (1960) The paired tracheal chain preparation. J Pharm Pharmacol 12: 189–191PubMedGoogle Scholar
  8. 8.
    McDougal MD, West GB (1953) The action of drugs on isolated mammalian bronchial muscle. Br J Pharmacol 8: 26–29Google Scholar
  9. 9.
    Constantine JW (1965) The spirally cut tracheal strip preparation. J Pharm Pharmacol 17: 384–385PubMedGoogle Scholar
  10. 10.
    Chand N, Eyre P (1978) Pharmacological study of chicken airway smooth muscle. J Pharm Pharmacol 30: 432–435PubMedGoogle Scholar
  11. 11.
    De Jongste JC, van Strik R, Bonta IL, Kerrebijn KF (1985) Measurement of human small airway smooth muscle function in vitro with the bronchiolar strip preparation. J Pharm Methods 14: 111–118Google Scholar
  12. 12.
    Rosa LM, McDowall JS (1951) The action of local hormones on the isolated human bronchus. Acta Allergol 4: 293–304PubMedGoogle Scholar
  13. 13.
    Danko G, Tozzi S, Roth FE (1968) Determination of the adrenergic mechanism of the isolated guinea pig trachea utilizing a new technique. Fed Proc Fed Am Soc Exp Biol 27: 351Google Scholar
  14. 14.
    Stephens NL, Meyers JL, Cherniack RM (1968) Oxygen, carbon dioxide, H+ ion and bronchial length tension relationships. J Appl Physiol 25: 376–383Google Scholar
  15. 15.
    Sterling GM, Holst PE, Nadel JA (1972) Effect of CO2 and pH on bronchoconstriction caused by serotonin vs acetylcholine. J Appl Physiol 32: 39–43PubMedGoogle Scholar
  16. 16.
    Souhrada JF, Dickey DW (1976) Mechanical activities of trachea as measured in vitro and in vivo. Respir Physiol 26: 27–40PubMedGoogle Scholar
  17. 17.
    Advenier C, Freslon JL ( 1985 The guinea-pig isolated bronchus for the in vitro study of small calibre airway reactivity. Br J Pharmacol 86: 367–373PubMedGoogle Scholar
  18. 18.
    Hooker CS, Calkins PJ, Fleisch JH (1977) On the measurement of vascular and respiratory smooth muscle responses in vitro. Blood Vessels 14: 1–11PubMedGoogle Scholar
  19. 19.
    Jongejan RC, de Jongste JC, van Strik R, Raatgeep HR, Bonta IL, Kerrebijn KF (1988) Measurement of human small airway smooth muscle function in vitro. Comparison of bronchiolar strip and segments. J Pharmacol Methods 20: 135–142Google Scholar
  20. 20.
    Trendelemburg P (1912) Physiologische and Pharmakologische Untersuchungen an der isolierten Bronchialmuskulatur. Arch Exp Pathol Pharmakol 69: 79–107Google Scholar
  21. 21.
    Macht DI, Ting GC (1921) A study of antispasmodic drugs on the bronchus. J Pharmacol Exp Ther 18: 373–398Google Scholar
  22. 22.
    Gaddum JH, Stephenson RP (1958) A microbath. Br J Pharmacol 13: 394–497Google Scholar
  23. 23.
    Offermeier J, Ariens EJ (1966) Serotonin. 1. Receptors involved in its action. Arch Int Pharmacodyn Ther 164: 192–215Google Scholar
  24. 24.
    Sperlakis N (1962) Contraction of depolarized smooth muscle by electric fields Am J Physiol 202: 731–742Google Scholar
  25. 25.
    Stephens NL, Kroeger E, Mehta JA (1969) Force-velocity characteristics of respiratory airway smooth muscle. J Appl Physiol 26: 685–692PubMedGoogle Scholar
  26. 26.
    Stephens NL (1975) Physical properties of contractile systems. Methods Pharmacol 3: 265–296Google Scholar
  27. 27.
    Coburn RF, Tomita T (1973) Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle. Am J Physiol 224: 1072–1080PubMedGoogle Scholar
  28. 28.
    Kirkpatrick CT (1975) Excitation and contraction in bovine tracheal smooth muscle. J Physiol 244: 263–281PubMedGoogle Scholar
  29. 29.
    Coburn RF, Yamaguchi T (1977) Membrane potential-dependent and -independent tension in the canine tracheal muscle. J Pharmacol Exp Ther 201: 276–284PubMedGoogle Scholar
  30. 30.
    Cameron AR, Kirkpatrick CT (1977) A study of excitatory neuromuscular transmission in the bovine trachea. J Physiol 270: 733–745PubMedGoogle Scholar
  31. 31.
    Yamaguchi T, Hitzig B, Coburn RF (1976) Endogeneous prostaglandins and mechanical tension in canine trachealis muscle. Am J Physiol 230: 1737–1743PubMedGoogle Scholar
  32. 32.
    Bose R, Bose D (1977) Excitation-contraction coupling in multiunit tracheal smooth muscle during metabolic depletion: induction of rhythmicity. Am J Physiol 2: C8 - C13Google Scholar
  33. 33.
    Lulich KM, Mitchell HW, Sparrow MP (1976) The cat lung strip as an in vitro preparation of peripheral airways: a comparison of ß-adrenoceptor agonists, autacoids and anaphylactic challenge on the lung strip and trachea. Br J Pharmacol 58: 71–79PubMedGoogle Scholar
  34. 34.
    Chand N, de Roth L (1979) Responses of guinea-pig lung parenchimal strips to prostaglandins and some selected autacoids. J Pharm Pharmacol 31: 712–714PubMedGoogle Scholar
  35. 35.
    Siegl PKS, Rossi GV, Orzechowski RF (1979) Isolated lung strips of guinea pigs.Responses to beta-adrenergic agonists and antagonists. Eur J Pharmacol 54: 1–7PubMedGoogle Scholar
  36. 36.
    Burn JW, Doe JE (1978) A comparison of drug-induced responses on rat tracheal bronchial and lung strip in vitro preparations. Br J Pharmacol 64: 71–74Google Scholar
  37. 37.
    Lulich KM, Paterson JW (1980) An in vitro study of various drugs on central and peripheral airways of the rat: a comparison with human airways. Br J Pharmacol 68: 633–636PubMedGoogle Scholar
  38. 38.
    Chand N (1981) Reactivity of isolated trachea, bronchus and lung strip of cats to carbachol, 5-hydroxytryptamine and histamine; evidence for the existence of methysergide-sensitive receptors. Br J Pharmacol 73: 853–857PubMedGoogle Scholar
  39. 39.
    Chand N, de Roth L, Eyre P (1979) Pharmacology of Schultz-Dale reaction in canine lung strip in vitro: possible model for allergic asthma Br J Pharmacol 66: 511–516Google Scholar
  40. 40.
    Goldie RG, Paterson JW, Wale JL (1982) Pharmacological responses of human and porcine lung parenchima, bronchus and pulmonary artery. Br J Pharmacol 76: 515–521PubMedGoogle Scholar
  41. 41.
    Mirbahar KB, Eyre P (1980) Bovine lung parenchyma strip has both airway and vascular characteristics ( Pharmacological comparison with bronchus, pulmonary artery and vein ). Res Commun Chem Pathol Pharmacol 29: 15–25Google Scholar
  42. 42.
    Ghelani AM, Holroyde MC, Sheard P (1980) Response of human isolated bronchial and lung parenchimal strips to SRS-A and other mediators of asthmatic bronchospasm. Br J Pharmacol 71: 107–112PubMedGoogle Scholar
  43. 43.
    Drazen JM, Schneider MW (1978) Comparative responses of tracheal spirals and parenchimal strips to histamine and carbachol in vitro. J Clin Invest 61: 1441–1448PubMedGoogle Scholar
  44. 44.
    Kapanci Y, Assimacopoulos A, Irle C, Zwahlen A, Gabbiani G (1974) Contractile interstitial cells in pulmonary alveolar septa: a possible regulator of ventilation/perfusion ratio? J Cell Biol 60: 375–392PubMedGoogle Scholar
  45. 45.
    Goldie RG, Bertram JF, Papadimitriou JM, Paterson JW (1984) The lung parenchyma strip. Trends Pharmacol Sci 5: 7–10Google Scholar
  46. 46.
    Eyre P, Mirbahar KN (1981) Is the lung parenchyma strip a true airway preparation? Agents Actions 11: 173–177PubMedGoogle Scholar
  47. 47.
    Bertram JF, Goldie RG, Papadimitriou JM, Paterson JW (1983) Correlation between pharmacological responses and structure of human lung parenchyma strips. Br J Pharmacol 80: 107–114PubMedGoogle Scholar
  48. 48.
    Farmer JB, Coleman RA (1970) A new preparation of the isolated intact trachea of the guinea-pig. J Pharm Pharmacol 22: 46–50PubMedGoogle Scholar
  49. 49.
    Carlyle RF (1964) The response of the guinea pig isolated intact trachea to trans-mural stimulation and the release of an acetylcholine-like substance under conditions of rest and stimulation. Br J Pharmacol 22: 126–136Google Scholar
  50. 50.
    Finkleman B (1930) On the nature of inhibition in the intestine. J Physiol 70: 145–147PubMedGoogle Scholar
  51. 51.
    Gaddum JH (1953) The technique of superfusion. Br J Pharmacol Chemother 8: 321PubMedGoogle Scholar
  52. 52.
    Coleman RA, Nials AT (1989) Novel and versatile superfusion system. J Pharmacol Methods 21: 71–86PubMedGoogle Scholar
  53. 53.
    Bouhuys A (1974) Bronchial asthma. In: Bouhuys A (ed) Breathing, physiology, environment and lung disease. Grune and Stratton, New York, pp 441–489Google Scholar
  54. 54.
    Armitage AK, Vane JR (1964) A sensitive method for the assay of catecholamines. Br J Pharmacol Chemother 22: 204–210PubMedGoogle Scholar
  55. 55.
    Henman MC, Naylor IL, Leach GDH (1978) A critical evaluation of the use of a cascade superfusion technique for the detection and estimation of biological activity. J Pharmacol Methods 1: 13–26Google Scholar
  56. 56.
    Willis AL (1969) Parallel assay of prostaglandine-like activity in rat inflammatory exudate by means of cascade superfusion. J Pharm Pharmacol 1: 126–128Google Scholar
  57. 57.
    Naylor IL (1977) A simple and inexpensive piece of apparatus for cascade super-fusion procedures. Br J Pharmacol 59: 529 PGoogle Scholar
  58. 58.
    Hong E (1974) Differential pattern of activity of some prostaglandins in diverse superfused tissues. Prostaglandins 8: 213–220PubMedGoogle Scholar
  59. 59.
    Gilmore N, Vane JR, Wyllie JH (1968) Prostaglandins released by the spleen. Nature 218: 1125–1140Google Scholar
  60. 60.
    Widmark, E, Waldeck B ( 1986 Physiological and pharmacological characterization of an in vitro vagus nerve-trachea preparation from guinea-pig. J Auton Pharmacol 6: 187–194PubMedGoogle Scholar
  61. 61.
    Empey DW, Laitinen LA, Jacobs L, Gold WM, Nadel JA (1976) Mechanisms of bronchial hyperreactivity in normal subjects following upper respiratory tract infection. Am Rev Respir Dis 113: 131–139PubMedGoogle Scholar
  62. 62.
    Farmer SG (1986) Neutrophils and ozone-induced airway hyper-reactivity: cause or effect? Trends Pharmacol Sci 7: 169–170Google Scholar
  63. 63.
    Farmer SG (1987) Airway smooth muscle responsiveness modulation by the epithelium. Trends Pharmacol Sci 81: 8–10Google Scholar
  64. 64.
    Flavahan NA, Aarhus T, Rimele TJ, van Houtte PM (1985) Respiratory epithelium inhibits bronchial smooth muscle tone. J Appl Physiol 58: 834–838PubMedGoogle Scholar
  65. 65.
    de Mey JG, van Houtte PM (1981) Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries. J Physiol 316: 347–355PubMedGoogle Scholar
  66. 66.
    Furchgott RF, Zawdazki JV (1980) The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288: 373–376PubMedGoogle Scholar
  67. 67.
    Hisayama T, Takayanagi I, Nakazato F, Hirano F (1988) Epithelium selectively controls hypersensitization of the response of smooth muscle to leukotriene D4 by endogenous prostanoid(s) in guinea-pig trachea. Naunyn-Schmiedebergs Arch Pharmacol 337: 296–300PubMedGoogle Scholar
  68. 68.
    Goldie RG, Papadimitrious JM, Paterson JW, Rigby PJ, Self HM, Spina D (1986) Influence of the epithelium on the responsiveness of guinea-pig isolated trachea to contractile and relaxant agonists. Br J Pharmacol 87: 5–14PubMedGoogle Scholar
  69. 69.
    Hay DWP, Farmer SG, Raeburn D, Robinson VA, Fleming WW, Fedan JS (1986) Airway epithelium modulates the reactivity of guinea-pig respiratory smooth muscle. Eur J Pharmacol 129: 11–18PubMedGoogle Scholar
  70. 70.
    Holroyde MC (1986) The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol 87: 501–507PubMedGoogle Scholar
  71. 71.
    Hay DWP, Raeburn D, Farmer SG, Fleming WW, Fedant JS (1986) Epithelium modulates the reactivity of ovalbumin-sensitized guinea-pig airway smooth muscle. Life Sci 38: 2461–2468PubMedGoogle Scholar
  72. 72.
    Raeburn D, Hay DWP, Robinson VA, Farmer SG, Fleming WW, Fedan JS (1986) The effect of verapamil is reduced in isolated airway smooth muscle preparations lacking the epithelium. Life Sci 38: 809–816PubMedGoogle Scholar
  73. 73.
    Barnes PJ, Cuss FM, Palmer JB (1985) The effect of airway epithelium on smooth muscle contractility in bovine trachea. Br J Pharmacol 86: 685–692PubMedGoogle Scholar
  74. 74.
    Raeburn D, Hay DWP, Farmer SG, Fedan JS (1986) Epithelium removal increases the reactivity of human isolated tracheal muscle to methacholine and reduces the effect of verapamil. Eur J Pharmacol 123: 451–453PubMedGoogle Scholar
  75. 75.
    Fine JM, Gordon T, Sheppard D (1989) Epithelium removal alters responsiveness of guinea-pig trachea to substance P. J Appl Physiol 66: 232–237PubMedGoogle Scholar
  76. 76.
    Aarrhus LL, Rimele TJ, van Houtte PM (1984) Removal of epithelium causes bronchial supersensitivity to acetylcholine and 5-hydroxytryptamine. Fed Proc 43: 995–999Google Scholar
  77. 77.
    Niemeier RW, Bingham E (1972) An isolated perfused lung preparation for metabolic studies. Life Sci 11: 807–820Google Scholar
  78. 78.
    Mehendale HM, Angevine LS, Ohmiya Y (1981) The isolated perfused lung: a critical evaluation. Toxicology 21: 1–36PubMedGoogle Scholar
  79. 79.
    Niemeier RW (1984) The isolated perfused lung. Environ Health Perspect 56: 35–41PubMedGoogle Scholar
  80. 80.
    Kroll F, Karlsson JA, Nilsson E, Persson CGA, Ryrfeldt A (1986) Lung mechanics of the guinea-pig isolated perfused lung. Acta Physiol Scand 128: 1–8PubMedGoogle Scholar
  81. 81.
    McLaughlin RJ (1977) Systematic design of cantilever beams for muscle research. J Appl Physiol 42: 786–794PubMedGoogle Scholar
  82. 82.
    Norris G, Carmeci P (1965) Isotonic muscle transducer. J Appl Physiol 20: 355–356Google Scholar
  83. 83.
    Illingworth DR, Naylor IL (1982) An improved, ultrasonic auxotonic transducer for use with isolated tissues. J Pharmacol Methods 8: 59–71PubMedGoogle Scholar
  84. a.Blattner R, Classen HG, Dehnert H, Döring HJ (1978) Experiments on isolated smooth muscle preparations. HSE Biological Measuring Techniques. Sachs, Freiburg, p4Google Scholar
  85. 84.
    Paton WDM (1975) The recording of mechanical responses of smooth muscle. Methods Pharmacol 3: 261–264Google Scholar
  86. 85.
    Santesson CG (1891) Studien über die allgemeine Mechanik des Muskels. Skand Arch Physiol 3: 381–436Google Scholar
  87. 86.
    Paton WDM (1957) A pendulum auxotonic lever. J Physiol 137: 35P - 36 PPubMedGoogle Scholar
  88. 87.
    Von Frey N (1908) Allegemeine muskelmechanik. In: Tigersted R (ed) Handbuch der physiologischen Methodik, vol 2. Hirzel, Leipzig, pp 87–119Google Scholar
  89. 88.
    Gaddum JH (1965) An improved microbath. Br J Pharmacol 23: 613–619Google Scholar
  90. 89.
    Jewell BR, Wilkie DR (1958) An analysis of the mechanical components in frog’s striated muscle. J Physiol 143: 515–540PubMedGoogle Scholar
  91. 90.
    Lewis AF (1969) An improved isotonic transducer. J Physiol 203: 15–17 PGoogle Scholar
  92. 91.
    Mc Intosh M, Duggan DE, Watt DD, Goodson LH (1965) A photopotentiometric transducer for detecting in vitro muscle contractions. J Appl Physiol 20: 349–350Google Scholar
  93. 92.
    Ross SM, Brust M (1966) A photopotentiometric isotonic myogram. J Appl Physiol 21: 293–294PubMedGoogle Scholar
  94. 93.
    Deby G, Espreux G, Topa C (1972) Appareil d’enregistrement electrique des contractions musculaires de faible amplitude. Experientia 28: 114–115PubMedGoogle Scholar
  95. 94.
    Bucking J, Herbst M, Piontek P (1973) Ein optischer Messwertwandler zur Registrierung der Muskelkontraktion. Experientia 29: 1311–1312PubMedGoogle Scholar
  96. 95.
    Erdos EG, Jackman V, Barnes WC (1962) Instrument for recording isotonic contractions of smooth muscles. J Appl Physiol 17: 367–368PubMedGoogle Scholar
  97. 96.
    Hansom DN, Hughes IE, Letley E (1970) An isotonic transducer for general use. J Pharm Pharmacol 22: 309–310Google Scholar
  98. 97.
    Jewell BR, Kretzschmar M, Woledge RC (1967) Length and tension transducer. J Physiol 191: 10P - 12 PPubMedGoogle Scholar
  99. 98.
    Mellor PM (1984) A precision isotonic measuring system for isolated tissues. J Pharmacol Methods 12: 259–264PubMedGoogle Scholar
  100. 99.
    Boucek RJ, Murphy WP, Paff GA (1959) Electrical and mechanical properties of chick embryo heart chambers. Circ Res 7: 787–793PubMedGoogle Scholar
  101. 100.
    Cambridge GW, Haines J (1959) A new versatile transducer system inductance/capacitance change. J Physiol 149: 2P - 3 PGoogle Scholar
  102. 101.
    Schilling MO (1960) Capacitance transducer for muscle research. Rev Sci Instr 31: 1215–1217Google Scholar
  103. 102.
    Duxbury AJ (1971) A simple linear transducer for physiological and pharmacological applications. Med Biol Eng 9: 719–720PubMedGoogle Scholar
  104. 103.
    Lewis AF (1969) An isometric strain-gauge transducer unit. J Physiol 203: 17P - 19 PPubMedGoogle Scholar
  105. 104.
    Feigl EO, Simon GA, Fry DL (1967) Auxotonic and isometric cardiac force transducers. J Appl Physiol 23: 597–600PubMedGoogle Scholar
  106. 105.
    Hedlund L, Valentich F, Ralph CL, Cepko J, Lynch HJ (1971) Serotonin assay: a tissue chamber and recording lever for the fundus strip method. J Appl Physiol 30: 787–791PubMedGoogle Scholar
  107. 106.
    Rosenblueth A, Alanis J, Robio R (1958) A comparative study of the isometric and isotonic contractions of striated muscle. Arch Int Physiol Biochem 66: 330–353Google Scholar
  108. 107.
    Taylor RR (1970) Active length-tension relations compared in isometric, after-load and isotonic contractions of cat papillary muscle–their dependence on inotropic state. Circ Res 26: 279–288PubMedGoogle Scholar
  109. 108.
    Michelson MJ, Shelkovnikov SA (1976) Isotonic and isometric responses of different tonic muscles to agonists and antagonists. Br J Pharmacol 56: 457–467PubMedGoogle Scholar
  110. 109.
    Stephens NL, Van Niekerk W (1976) Isometric and isotonic contractions in airway smooth muscle. Can J Physiol Pharmacol 55: 833–838Google Scholar
  111. 110.
    De Jongste JC, Mons H, van Strik R, Bonta IL, Kerrebijn KF (1987) Comparison of isometric and isotonic responses of human small airway smooth muscle in vitro. J Pharmacol Methods 17: 165–171PubMedGoogle Scholar
  112. 111.
    Christensen JH, ostgaard SE, Andreasen F (1982) The influence of pO2i pH and albumin on the in vitro contraction of vascular smooth muscle. J Pharmacol Methods 8: 99–108PubMedGoogle Scholar
  113. 112.
    Lockwood APM (1961) Ringer solutions and some notes on the physiological basis of their ionic compositions. Comp Biochem Physiol 2: 241–289PubMedGoogle Scholar
  114. 113.
    Perry WLM (1968) Pharmacological experiments on isolated preparations. Livingstone, EdinburghGoogle Scholar
  115. 114.
    Mac Conail M (1985) Calcium precipitation from mammalian physiological salines (Ringer solutions) and the preparation of high [Ca] media. J Pharmacol Methods 14: 147–155Google Scholar
  116. 115.
    Chang AA, Detar R (1980) Oxygen and vascular smooth muscle contraction revisited. Am J Physiol 238: H716 - H728PubMedGoogle Scholar
  117. 116.
    Stephens NL, Meyer JL, Cherniack RM (1968) Oxygen, carbon dioxide, H+ ion, and bronchial length-tension relationships. J Appl Physiol 25: 376–383Google Scholar
  118. 117.
    Ringer S (1883) A further contribution regarding the influence of the different constituents of the blood on the contraction of the heart. J Physiol 4: 249–254Google Scholar
  119. 118.
    Locke FS, Rosenheim O (1907) Contributions to the physiology of the isolated heart. The consumption of dextrose by mammalian cardiac muscle. J Physiol 36: 205–220Google Scholar
  120. 119.
    Chenoweth MB, Koelle ES (1946) An isolated heart perfusion system adapted to the determination of nongasesous metabolites. J Lab Clin Med 31: 600–608PubMedGoogle Scholar
  121. 120.
    Goodford PJ, Freeman-Narrod M (1962) Sodium and potassium content of the smooth muscle of the guinea-pig taenia coli at different temperatures and tensions. J Physiol 163: 399–410PubMedGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • P. C. Braga
    • 1
  1. 1.Center for Respiratory Pharmacology, School of MedicineUniversity of MilanMilanoItaly

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