Immunology of Chlamydia pneumoniae

  • Maija Leinonen
  • Aino Laurila
  • Kirsi Laitinen
  • Heljä M. Surcel


All chlamydia are obligate intracellular parasites and at their simplest can be regarded as highly specialized gram-negative bacteria with two developmental forms: infective elementary bodies and reproductive reticulate bodies. Both the structural components of the chlamydial cell (virulence factors) and the host cell factors — how the host resists to chlamydial infection — play a role in the immunological mechanisms associated with chlamydial infections. To date very little is known about the immunology of C. pneumoniae,but evidently most immunological mechanisms associated with other chlamydial infections are also involved in C. pneumoniae infections.


Chlamydia Trachomatis Chlamydial Infection Obligate Intracellular Parasite Chlamydial Species Lymphogranuloma Venereum 
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  1. 1.
    Black CM, Perez R (1990) Chlamydia pneumoniae multiplies within pulmonary macrophages. In: Abstr 90th Annual Meet A.S.M., Anaheim,1990; abstr D-1, Amer Soc Microbiol, Washington DC, 82Google Scholar
  2. 2.
    Kaukoranta-Tolvanen SS, Saikku P, Leinonen M (1992) Growth of Chlamydia pneumoniae in endothelial cells compared to other cell types Proc European Soc for Chlamydia Res 2: 168Google Scholar
  3. 3.
    Blasi F, Legnani D, Lombardo VM, Negretto GG, Magliano E, Pozzoli R, Chiodo F, Fasoli A, Allegra L (1993) Chlamydia pneumoniae infection in acute exacerbations of COPD. Eur Respir J 6: 19–22Google Scholar
  4. 4.
    von Herzen L, Leinonen M, Koskinen R, Liippo K, Saikku P (1994) Evidence of persistent Chlamydia pneumoniae infection in patients with chronic obstructive pulmonary disease. In: Proceedings of the 8th International Symposium on Human Chlamydial infections, Chantilly 19–24 June 1994, Orfila J et al (eds), Esculapio Bologna, pp 473–476Google Scholar
  5. 5.
    Hahn DL, Dodge RW, Golubjatnikov R (1991) Association of Chlamydia pneumoniae (strain TWAR) infection with wheezing, asthmatic bronchitis, and adult-onset asthma. JAMA 266: 225–230PubMedCrossRefGoogle Scholar
  6. 6.
    Grönhagen-Riska C, Saikku P, Riska H, Fröseth B, Grayston JT (1988) Antibodies to TWAR–a novel Type of Chlamydia - in sarcoidosis. In: Sarcoidosis and other granuloma-tous disorders. Grassi C et al (eds), Elsevier Scientific Publ, Amsterdam, pp 297–301Google Scholar
  7. 7.
    Black CM, Bullard JC, Staton GW, Hutwagner LC, Perez RL (1992) Seroprevalence of Chlamydia pneumoniae antibodies in patients with pulmonary sarcoidosis in North Central Georgia. Proc European Soc for Chlamydia Res 2: 175Google Scholar
  8. 8.
    Saikku P, Mattila K, Nieminen MS, Huttunen JK, Leinonen M, Ekman MR, Mäkelä PH, Valtonen V (1988) Serological evidence of an association of a novel Chlamydia, TWAR, with chronic heart disease and acute myocardial infarction. Lancet ii: 983–985Google Scholar
  9. 9.
    Saikku P, Leinonen M, Tenkanen L, Ekman MR, Linnanmärki E, Manninen V, Mänttäri M, Frick MM, Huttunen JK (1992) Chronic Chlamydia pneumoniae infection as a risk factor for coronary heart disease in the Helsinki Heart Study. Ann Int Med 116: 273–278PubMedGoogle Scholar
  10. 10.
    Kuo CC, Shor A, Campbell LA, Fukushi H, Patton DL, Grayston JT (1993) Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J Infect Dis 167: 845–849CrossRefGoogle Scholar
  11. 11.
    Nurminen M; Rietschel ET, Brade H (1985) Chemical characterization of Chlamydia trachomatis lipopolysaccaride. Infect Immun 48: 573–575PubMedGoogle Scholar
  12. 12.
    Brade L, Schramek S, Schade U, Brade H (1986) Chemical, biological and immunochemical properties of Chlamydia psittaci lipopolysaccharide. Infec Immun 54: 567–574Google Scholar
  13. 13.
    Leinonen M, Linnanmäki E, Mattila K, Nieminen MS, Leirisalo-Repo M, Valtonen V, Saikku P (1990) Circulating immune complexes containing Chlamydial lypopolisaccharide in acute myocardial infarction. Microbial Patoghenesis 9: 67–73CrossRefGoogle Scholar
  14. 14.
    Linnanmärki E, Leinonen M, Ekman MR, Mattila K, Nieminen MS, Valtonen V, Saikkui P (1993) Chlamydia pneumoniae specific circulating immune complexes in chronic heart disease. Circulation 87: 1130–1134Google Scholar
  15. 15.
    Gaydos CA, Quinn TC, Bobo LD, Eiden JJ (1992) Similarity of Chlamydia pneumoniae strains in the variable domain IV region of the major outer membrane protein gene. Infect Immun 60: 5319–5323PubMedGoogle Scholar
  16. 16.
    Black CM, Johnson JE, Farshy CE, Brown TM, Berdal BP (1991) Antigenic variation among strains of Chlamydia pneumoniae. J Clin Microbiol 29: 1312–1316PubMedGoogle Scholar
  17. 17.
    Lindquist S, Craig EA (1988) The heat shock proteins. Ann Rev Genet 22: 631–677PubMedCrossRefGoogle Scholar
  18. 18.
    Young DB, Ivanyi J, Cox JH, Lamb JR (1987) The 65 kDa antigen of mycobacteria-a common bacterial protein. Immunol Today 8: 215–219CrossRefGoogle Scholar
  19. 19.
    Young D, Lathigra R, Hendrix R, Sweetser R, Young RA (1988) Stress proteins are immune targets in leprosy and tuberculosis. Proc Natl Acad Sci 85: 4267–4270PubMedCrossRefGoogle Scholar
  20. 20.
    Menozzi FD, Menozzi-Dejaiffe C, Nano FE (1989) Molecular cloning of a gene encoding a Chlamydia psittaci 57 kDa protein that shares antigenic determinants with ca. 60 kDa proteins present in many gram-negative bacteria. FEBS Letters 58: 59–64Google Scholar
  21. 21.
    Cerrone MC, Ma JJ, Stephens RS (1991) Cloning and sequence of the gene for heat shock protein 60 from Chlamydia trachomatis and immunological reactivity of the protein. Infect Immun 59: 79–90PubMedGoogle Scholar
  22. 22.
    Birkelund S, Lundemose AG, Christiansen G (1990) The 75 kDa cytoplasmatioc Chlamydia trachomatis L“2 polypeptide is a DNA-like protein. Infect Immun 58: 2098–2104PubMedGoogle Scholar
  23. 23.
    Grayston JT, Campbell LA, Kuo CC, Mordhorst CH, Saikku P, Thom DH, Wang SPGoogle Scholar
  24. (1990).
    A new respiratory tract pathogen, Chlamydia pneumoniae strain TWAR. J Infect Dis 161:618–625Google Scholar
  25. 24.
    Ekman MR, Grayston JT, Visakorpi R, Kleemola M, Kuo CC, Saikku P (1993) An epidemic of infections due to Chlamydia pneumoniae in military conscripts. Clin Infect Dis 17: 420–425PubMedCrossRefGoogle Scholar
  26. 25.
    Ekman MR, Leinonen M, Syrjälä H, Linnanmäki E, Kujala P, Saikku P (1993) Evaluation of serological methods in the diagnosis of Chlamydia pneumoniae during an epidemic in Finland. Eur J Clin Microbiol 12: 756–760CrossRefGoogle Scholar
  27. 26.
    Marrie TJ, Grayston JT, Kuo CC (1987) Pneumonia associated with TWAR strain of Chlamydia. Ann Int Med 106: 507–511PubMedGoogle Scholar
  28. 27.
    Campbell LA, Kuo CC, Wang SP, Grayston JT (1990) Serological response to Chlamydia pneumoniae infection. J Clin Microbiol 28: 1261–1264PubMedGoogle Scholar
  29. 28.
    Freidank HM, Herr AS, Jacobs E (1993) Identifications of Chlamydia pneumoniae specific protein antigens in immunoblots. Eur J Clin Microbiol Infect Dis 112: 947–951CrossRefGoogle Scholar
  30. 29.
    Iijima Y, Miyashita N, Kishimoto T, Kanamoto Y, Soejima R, Matsumoto A (1994) Characterization of Chlamydia pneumoniae species specific proteins immunodominant in humans. J Clin Microbiol 32: 583–588PubMedGoogle Scholar
  31. 30.
    Puolakkainen M, Kuo CC, Shor A, Wang SP, Grayston JT, Campbell LA (1993) Serological response to Chlamydia pneumoniae in adults with coronary arterial fatty streaks and fibrolipid plaques. J Clin Microbiol 31: 2212–2214PubMedGoogle Scholar
  32. 31.
    Forsey T, Darougar S, Treharne JD, Jones BR, Herring J (1986) Prevalence in human beings of antibodies to Chlamydia IOL-207, an atypical strain of Chlamydia. J Infect 12: 145–152PubMedCrossRefGoogle Scholar
  33. 32.
    Wang SP, Grayston JT (1990) Population prevalence antibody to Chlamydia pneumoniae, strain TWAR. In: Proceedings of the 7th International Symposium on Human Chlamydial Infections, Bowie WR et al (eds) pp 402–405Google Scholar
  34. 33.
    Saikku P (1992) The epidemiology and significance of Chlamydia pneumoniae. J Infect 25 (S1): 27–34PubMedCrossRefGoogle Scholar
  35. 34.
    Bruu AL, Aasen S, Tjaland S, Birkeland S, Flugsrud L (1984) An outbreak of ornithosis in Norway in 1981. Scand J Infect Dis 16: 145–152PubMedCrossRefGoogle Scholar
  36. 35.
    Grayston JT, Mordhorst CH, Bruu AL, Vene S, Wang SP (1989) Countrywide epoidemics of Chlamydia pneumoniae, strain TWAR, in Scandinavia, 1981–1983. J Infect Dis 159: 11111114Google Scholar
  37. 36.
    Kleemola M, Saikku P, Viasakorpi R, Wang SP, Grayston JT (1988) Epidemics of pneumonia caused by TWAR, a new Chlamydia organism, in military trainees in Finland J Infect Dis 157: 230–236Google Scholar
  38. 37.
    Töyrylä M, Isoaho R, Puolijoki H, Huhti E, Kivelä SL, Saikku P (1992) Prevalence of Chlamydia pneumoniae antibodies of enderly population in Finnish rural district. Proc European Soc for Chlamydia Res 2: 293Google Scholar
  39. 38.
    Patnode D, Wang SP, Grayston JT (1990) Persistence of Chlamydia pneumoniae, strain TWAR, microimmunofluorescent antibody. In: Proceedings of the 7th International Symposium on Human Chlamydial Infections, Bowie WR et al (eds) pp 406–409Google Scholar
  40. 39.
    Grayston JT, Woolridge RL, Wang SP (1962) Trachoma vaccine studies on Taiwan. Ann N.Y. Acad Sci 98: 352–367Google Scholar
  41. 40.
    Grayston JT, Wang SP, Yreh LJ, Kuo CC (1985) Importance of reinfection in the pathogenesis of trachoma. Rev Inf Dis 7: 717–725CrossRefGoogle Scholar
  42. 41.
    Wang SP, Grayston JT, Alexander ER (1967) Trachoma vaccine studies in monkeys. Am J Ophtalmol 63: 1615–1630Google Scholar
  43. 42.
    Watkins NG, Hadlow WJ, Moos AB, Caldwell HD (1986) Ocular delayed bypersensitivity: apathogenetic mechanism of Chlamydial conjunctivitis in guinea pig. Proc Natl Acad Sci 83: 7480–7487PubMedCrossRefGoogle Scholar
  44. 43.
    Moller BR, Weström L, Ahrons S, Ripa T, Swensson L, Mecklelburg C, Henrikson H, Märdh Pa (1979) Chlamydia trachomatis infection of the fallopian tubes. Br J Vener Dis 55: 422–429Google Scholar
  45. 44.
    Morrison RP, Belland RJ, Lyng K, Calwell HD (1989) Chlamydial hypersensitivity antigen is a stress response protein. J Exp Med 170: 1271–1283PubMedCrossRefGoogle Scholar
  46. 45.
    Brunham RC, Peeling R, Maclean I, McDowell J, Persson K, Osser S (1987) Postabortal Chlamydia trachomatis salpingitis: correlating risk with antigen-specific serological responses and with neutralization. J Infect Dis 155: 749–755PubMedCrossRefGoogle Scholar
  47. 46.
    Morrison RP (1990) Chlamydial 57 kDa stress response protein is deleterious immune target. In: Microbial determinants of virulence and host response. Ayuoub EM et al (eds) American Society of Microbiology, Washington DC, 243–250Google Scholar
  48. 47.
    Bavoil P, Stephens RS, Falkow S (1990) A soluble 60 kDa antigen of Chlamydia spp. is homologue of Escherichia coli GroEl. Molec Microbiol 4: 461–469CrossRefGoogle Scholar
  49. 48.
    Rank RGL, Soderberg LSF, Sanders MM, Batteiger B (1989) Role of cell mediated immunity in the resolution of secondary Chlamydial genital infection in guinea pigs infected with the agent of guinea pig inclusion conjunctivitis. Infect Immun 57: 706–710PubMedGoogle Scholar
  50. 49.
    Surcel HM, Syrjälä H, Leinonen M, Saikku P, Herva E (1993) Cell-mediated immunity to Chlamydia pneumoniae measured as lymphocytes blast transformation in vitro. Infect Immun 61: 2196–2199PubMedGoogle Scholar
  51. 50.
    Holtman H, Shemer-Avni Y, Wessel K, Sarov I, Wallach D (1990) Inhibition of growth of Chlamydia trachomatis by tumor necrosis factor is accompanied by increased prostaglandin synthesis. Infect Immun 58: 3168–3172Google Scholar
  52. 51.
    Williams DM, Magee DM, Bonewald LF, Smith JG, Bleicker CA, Byrne GI, Schacter J (1990) A role in vivo for tumor necrosis factor alpha in host defence against Chlamydia trachomatis. Infect Immun 58: 1572–1576PubMedGoogle Scholar
  53. 52.
    Kawakami M, Pekala PH, Lance MD, Cerami A (1983) Lipoprotein lipase supression in 3T3 Ll cells by an induced mediator from exudate cells. Proc Natl Acad Sci 79: 912–916CrossRefGoogle Scholar
  54. 53.
    Kaukoranta-Tolvanen SS, Teppo AM, Leinonen M, Saikku P, Laitinen K (1992) Chlamydia pneumoniae induces the production of TNFa, IL-lb, and IL-6 by human monocytes. Proc European Soc for Chlamydia Res 2: 85Google Scholar
  55. 54.
    Howard LV (1975) Neutralization of Chlamydia trachomatis in cell culture. Infect Immun 11: 698–703PubMedGoogle Scholar
  56. 55.
    Ainsworth S, Allan I, Pearce JH (1979) Differential neutralization of spontaneous and centrifuge assisted Chlamydial activity. J Gen Microbiol 114: 61–67PubMedGoogle Scholar
  57. 56.
    Williams DM, Schacter J, Coalson J, Grubbs B (1984) Cellular immunity to the mouse pneumonitis agent. J Infect Dis 149: 630–639PubMedCrossRefGoogle Scholar
  58. 57.
    Cui ZD, LaScolea U, Fischer J, Ogra PL (1989) Immunoprophylaxis of Chlamydia trachomatis lymphogranuloma venereum pneumonitis in mice by oral immunizations. Infect Immun 57: 739–744PubMedGoogle Scholar
  59. 58.
    Williams DM, Schacter J, Drutz DJ, Sumaya CV (1981) Pneumonia due to Chlamydia trachomatis in the immunocompromized (nude) mouse. J Infect Dis 143: 238–241PubMedCrossRefGoogle Scholar
  60. 59.
    Rank RG, Soderberg LS, Barron Al (1985) Chronic Chlamydial infection in congenitally athymic mice. Infect Immun 48: 847–849PubMedGoogle Scholar
  61. 60.
    Ramsey KH, Rank RG (1991) Resolution of Chlamydial genital infection with antigen specific T-lymphocyte lines. Infect Immun 59: 925–931PubMedGoogle Scholar
  62. 61.
    Romagnani S (1992) Induction of TH1 and TH2 responses: a key role for the natural immune response. Immunology Today 13: 379–381PubMedCrossRefGoogle Scholar
  63. 62.
    Igiewtseme JU, Rank RG (1991) Susceptibility to reinfection after a primary Chlamydial genital infection is associated with a decrease of antigen specific T -cells in the genital tract. Infect Immun 59: 1346–1351Google Scholar
  64. 63.
    Yang ZP, Kuo CC, Grayston JT (1993) A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis. Infect Immun 61: 2037–2040PubMedGoogle Scholar
  65. 64.
    Kaukoranta-Tolvanen SS, Laurila Al, Saikku P, Leinonen M, Liesirova L, Laitinen K (1993) Experimental infection of Chlamydia pneumoniae in mice. Microbial Pathogenesis 15: 293–302PubMedCrossRefGoogle Scholar
  66. 65.
    Laitinen K, Laurila A, Leinonen M, Saikku P (1994) Experimental Chlamydia pneumoniae infection in mice: effect of reinfection and passive protection by immune serum. In: Proceedings of the 8th International Symposium on Human Chlamydial infections, Chantilly 19–24 June 1994, Orfila J et al (eds) Esculapio, Bologna, pp 545–548Google Scholar
  67. 66.
    Yang YS, Kuo CC, Chen WJ (1983) Reactivation of Chlamydia trachomatis lung infection in mice by cortisone. Infect Immun 39: 655–658PubMedGoogle Scholar

Copyright information

© Springer-Verlag Italia, Milano 1995

Authors and Affiliations

  • Maija Leinonen
  • Aino Laurila
  • Kirsi Laitinen
  • Heljä M. Surcel

There are no affiliations available

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