Metallothionein in Antarctic Fish

  • Vincenzo Carginale
  • Antonio Capasso
  • Clemente Capasso
  • Peter Kille
  • Elio Parisi
  • Gianluca L. Passaretti
  • Guido di Prisco
  • Marilisa Riggio
  • Rosaria Scudiero


Zinc occupies a prominent position among transition and d10 elements because of the role played in many biological processes [1,2], including catalysis [3], transcription and translation [4]. The functional role of zinc depends on the large variety of metallorganic complexes produced by this element as a result of its interaction with different protein families [3,5].


Leader Sequence Antarctic Fish Cysteine Content Stone Loach Mitochondrial Calcium Uniporter 
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  1. 1.
    Danks DM (1986) Of mice and men, metals and mutations. J Med Genet 23:99–106PubMedCrossRefGoogle Scholar
  2. 2.
    Prasad AS (1991) Discovery of human zinc deficiency and studies in an experimental human model. Am J Clin Nutr 53:403–412PubMedGoogle Scholar
  3. 3.
    O’Dell BL (1992) Zinc plays both structural and catalytic roles in metalloproteins. Nutr Rev 50:48–50CrossRefGoogle Scholar
  4. 4.
    Giedroc DP, Keating KM, Martin CT, Williams KR, Coleman JE (1986) Zinc metalloproteins involved in replication and transcription. J Inorg Biochem 28:155–169PubMedCrossRefGoogle Scholar
  5. 5.
    Vallee BL, Auld DS (1995) Zinc metallochemistry in biochemistry. Exs 73:259–277PubMedGoogle Scholar
  6. 6.
    Coto JA, Hadden EM, Sauro M, Zorn N, Hadden JW (1992) Interleukin 1 regulates secretion of zinc-thymulin by human thymic epithelial cells and its action on T-lymphocyte proliferation and nuclear protein kinase C. Proc Natl Acad Sci U S A 89:7752–7756PubMedCrossRefGoogle Scholar
  7. 7.
    Saris NE, Niva K (1994) Is Zn2+ transported by the mitochondrial calcium uniporter? Febs Lett 356:195–198PubMedCrossRefGoogle Scholar
  8. 8.
    Kille P, Hemmings A, Lunney EA (1994) Memories of metallothionein. Biochim Biophys Acta 1205:151–161PubMedCrossRefGoogle Scholar
  9. 9.
    Vallee BL (1995) The function of metallothionein. Neurochem Int 27:23–33PubMedCrossRefGoogle Scholar
  10. 10.
    De SK, Dey SK, Andrews GK (1990) Cadmium teratogenicity and its relationship with metallothionein gene expression in midgestation mouse embryos. Toxicology 64:89–104PubMedCrossRefGoogle Scholar
  11. 11.
    Camhi SL, Lee P, Choi AM (1995) The oxidative stress response. New Horiz 3:170–182PubMedGoogle Scholar
  12. 12.
    Kagi JHR, Nordberg M (1979) Metallothionein. Birkhaser Verlag. BaselGoogle Scholar
  13. 13.
    Robbins AH, Stout CD (1991) X-ray structure of metallothionein. Methods Enzymol 205:485–502PubMedCrossRefGoogle Scholar
  14. 14.
    Narula SS, Brouwer M, Hua Y, Armitage IM (1995) Three-dimensional solution structure of Callinectes sapidus metallothionein-1 determined by homonuclear and heteronuclear magnetic resonance spectroscopy. Biochemistry 34:620–631PubMedCrossRefGoogle Scholar
  15. 15.
    Andersen RD, Piletz JE, Birren BW, Herschman HR (1983) Levels of metallothionein messenger RNA in foetal, neonatal and maternal rat liver. Eur J Biochem 131:497–500PubMedCrossRefGoogle Scholar
  16. 16.
    Bonham K, Gedamu L (1984) Induction of metallothionein and metallothionein mRNA in rainbow-trout liver following cadmium treatment. Biosci Rep 4:633–642PubMedCrossRefGoogle Scholar
  17. 17.
    George SG, Todd K, Wright J (1996) Regulation of metallothionein in teleosts: induction of MT mRNA and protein by cadmium in hepatic and extrahepatic tissues of a marine flatfish, the turbot (Scophthalmus maximus). Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 113:109–115PubMedCrossRefGoogle Scholar
  18. 18.
    Schwarz MA, Lazo JS, Yalowich JC, Allen WP, Whitmore M, Bergonia HA, Tzeng E, Billiar TR, Robbins PD, Lancaster J, Jr (1995) Metallothionein protects against the cytotoxic and DNA-damaging effects of nitric oxide. Proc Natl Acad Sci U S A 92:4452–4456PubMedCrossRefGoogle Scholar
  19. 19.
    Dalton T, Palmiter RD, Andrews GK (1994) Transcriptional induction of the mouse metallothionein-I gene in hydrogen peroxide-treated Hepa cells involves a composite major late transcription factor/antioxidant response element and metal response promoter elements. Nucleic Acids Res 22:5016–5023PubMedCrossRefGoogle Scholar
  20. 20.
    Xie T, Belinsky M, Xu Y, Jaiswal AK (1995) ARE-and TRE-mediated regulation of gene expression. Response to xenobiotics and antioxidants. J Biol Chem 270:6894–6900PubMedCrossRefGoogle Scholar
  21. 21.
    Lazo JS, Kondo Y, Dellapiazza D, Michalska AE, Choo KH, Pitt BR (1995) Enhanced sensitivity to oxidative stress in cultured embryonic cells from transgenic mice deficient in metallothionein I and II genes. J Biol Chem 270:5506–5510PubMedCrossRefGoogle Scholar
  22. 22.
    Kille P, Stephens PE, Kay J (1991) Elucidation of cDNA sequences for metallothioneins from rainbow trout, stone loach and pike liver using the polymerase chain reaction. Biochim Biophys Acta 1089:407–410PubMedCrossRefGoogle Scholar
  23. 23.
    Peitsch MC (1996) Promod and Swiss model, internet-bond tools for automated comparative protein modelling. Biochem Soc Trans 24:274–279PubMedGoogle Scholar
  24. 24.
    Scudiero R, De Prisco PP, Camardella L, D’Avino R, di Prisco G, Parisi E (1992) Apparent deficiency of metallothionein in the liver of the Antarctic icefish Chionodraco hamatus. Identification and isolation of a zinc-containing protein unlike metallothionein. Comp Biochem Physiol 103B:201–207Google Scholar
  25. 25.
    Scudiero R, Carginale V, Riggio M, Capasso C, Capasso A, Kille P, di Prisco G, Parisi E (1997) Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. Biochem J 322:207–211PubMedGoogle Scholar

Copyright information

© Springer-Verlag Italia 1998

Authors and Affiliations

  • Vincenzo Carginale
    • 1
  • Antonio Capasso
    • 1
  • Clemente Capasso
    • 1
  • Peter Kille
    • 2
  • Elio Parisi
    • 1
  • Gianluca L. Passaretti
    • 1
  • Guido di Prisco
    • 1
  • Marilisa Riggio
    • 1
  • Rosaria Scudiero
    • 1
  1. 1.Institute of Protein Biochemistry and EnzymologyCNRNapoliItaly
  2. 2.School of Molecular and Medical BiosciencesUniversity of WalesCardiffUK

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