Exploring Cerebral Metabolism by Microdialysis

  • L. Persson
  • L. Hillered
Conference paper


Microdialysis (MD) has been widely used for sampling of chemical substances from the interstitial fluid of the brain in experimental research. In the late 1980s, MD was introduced for studies on the human brain and neurochemical sampling was carried out in conjunction with neurosurgical procedures [1, 2, 3]. In 1992 we reported the use of intracerebral MD for chemical monitoring of brain injured patients during neurointensive care (NIC) [4]. Since then, there has been a remarkable development in the use of this technique in many neurosurgical centers. One may say that frequent chemical sampling, at the bed side, has brought NIC monitoring from a “physiological level” to a “chemical level”, and the term neurochemical monitoring has been coined to describe this development [5].


Cerebral Metabolism Brain Injured Patient Neurointensive Care Glutamate Aspartate Intracerebral Microdialysis 


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  1. 1.
    Persson L, Hillered L, Ponten U et al (1989) Intracerebral microdialysis for continuous metabolic monitoring of neurosurgical patients: Preliminary methodological considerations. J Cerebr Blood Flow Metab 9[Suppl 1]:584Google Scholar
  2. 2.
    Hillered L, Persson L, Ponten U, Ungerstedt U (1990) Neurometabolic monitoring of the ischemic human brain using microdialysis. Acta Neurochir 102:91–97PubMedCrossRefGoogle Scholar
  3. 3.
    Meyerson BA, Linderoth B, Karlsson H, Ungerstedt U (1990) Microdialysis in the human brain: extracellular measurements in the thalamus of parkinsonian patients. Life Sei 46: 301–308CrossRefGoogle Scholar
  4. 4.
    Persson L, Hillered L (1992) Chemical monitoring of neurosurgical intensive care patients using intracerebral microdialysis. J Neurosurg 76:72–80PubMedCrossRefGoogle Scholar
  5. 5.
    Persson L, Valtysson J, Enblad P et al (1996) Neurochemical monitoring of patients with subarachnoid hemorrhage with intracerebral microdialysis. J Neurosurg 84:606–616PubMedCrossRefGoogle Scholar
  6. 6.
    Goodman JC, Gopinath SP, Valadka AB et al (1996) Lactic acid and amino acid fluctuations measured using microdialysis reflect physiological derangements in head injury. Acta Neurochir 67[Suppl]: 37–39Google Scholar
  7. 7.
    Landolt H, Langemann H, Gratzl O (1993) On-line monitoring of cerebral pH by microdialysis. Neurosurgery 32:1000–1004PubMedCrossRefGoogle Scholar
  8. 8.
    Bullock R, Zauner A, Myseros JS et al (1995) Evidence for prolonged release of ecitatory amino acids in severe human head trauma. Relationship to clinical events. Ann NY Acad Sei 765:290–297CrossRefGoogle Scholar
  9. 9.
    Hamberger A, Runnerstam M, Nyström B et al (1995) The neuronal microenvironment after subarachnoid hemorrhage. Correlation of amino acid and nucleoside levels with post-operative recovery. Neurol Res 17:97–105Google Scholar
  10. 10.
    Kanthan R, Shuaib A, Griebel R, Miyashita H (1995) Intracerebral human microdialysis. In vivo study of an acute focal ischemic model of the human brain. Stroke 26:870–873PubMedCrossRefGoogle Scholar
  11. 11.
    Robertson CS, Gopinath SP, Goodman JC et al (1995) SjvO2 monitoring in head-injured patients. J Neurotrauma 12:891–896PubMedCrossRefGoogle Scholar
  12. 12.
    Säveland H, Nilsson OG, Boris-Möller F et al (1996) Intracerebral microdialysis of glutamate and aspartate in two vascular territories after aneurysmal subarachnoid hemorrhage. Neurosurgery 38:12–19PubMedCrossRefGoogle Scholar
  13. 13.
    Enblad P, Valtysson J, Andersson J et al (1996) Simultaneous intracerebral microdialysis and positron emission tomography performed in the detection of ischemia in patients with subarachnoid hemorrhage. J Cereb Blood Flow Metab 16:637–644PubMedCrossRefGoogle Scholar
  14. 14.
    Benveniste H (1989) Brain microdialysis. J Neurochem 52:1667–1679PubMedCrossRefGoogle Scholar
  15. 15.
    Robinson T, Justice JB (1991) Microdialysis in the neurosciences. Elsevier, AmsterdamGoogle Scholar
  16. 16.
    Hillered L, Valtysson J, Enblad P, Persson L (1997) Extracellular glycerol as a marker for membrane lipid degradation in the acutely injured human brain. J Neurol Neurosurg Psychiatry (in press)Google Scholar
  17. 17.
    Lönnroth P, Jansson P-A, Smith U (1987) A microdialysis method allowing characterization of intercellular water space in humans. Am J Physiol E253:E228–E231PubMedGoogle Scholar
  18. 18.
    Kehr J (1993) A survey on quantitative microdialysis: Theoretical models and practical implications. J Neurosci Methods 48:251–261PubMedCrossRefGoogle Scholar
  19. 19.
    Persson L, Hillered L (1996) Intracerebral microdialysis. Letter to the Editor. J Neurosurg 85: 984–985Google Scholar

Copyright information

© Springer-Verlag Italia 1999

Authors and Affiliations

  • L. Persson
    • 1
  • L. Hillered
    • 2
  1. 1.Dept. of NeurosurgeryUppsala University HospitalUppsalaSweden
  2. 2.Dept. of Clinical ChemistryUppsala University HospitalUppsalaSweden

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