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Enzymatic Activities and Carbon Flux through the Microbial Compartment in the Adriatic Sea

  • R. La Ferla
  • R. Zaccone
  • G. Caruso
  • M. Azzaro

Abstract

Carbon flux through the microbial community by the determination of biomass, heterotrophic bacteria, aminopeptidase and respiratory activities has been studied in two areas of the Adriatic sea with different trophic characteristics during four oceanographic surveys, carried out in June 96,97 and February 97,98. In front of the Po delta (area A), the average rates of the carbon released by aminopeptidase activity ranged from 4.9 to 9.9 μg Cl−1 h−1 and near the Ancona coast (area B) from 3.1 to 7.6μg Cl−1 h−1, whereas the microbial respiration as metabolic carbon production (CO2) ranged from 0.19 to 2.29 and from 0.24 to 1.40μg Cl−1 h−1 in the two areas, respectively.

In area A, both the microbial activities showed a seasonal trend with higher values in summer than in winter. In area B, respiration increased during years whilst aminopeptidase activity decreased; bacterioplankton abundance increased on average in the second year of the research (June 97 and February 98). However, all these differences were not statistically significant. The biovolume resulted always significantly higher during summer.

Relationships between bacterial carbon production and organic carbon content were discussed. Bacterial growth efficiency ranged from 17 to 38% and from 13 to 44% in areas A and B, respectively. The relationship between hydrolysis of peptides and bacterial carbon production indicated a good coupling of processes during June 97. Various ratios between bacterial activities were reckoned with the aim to define the role of bacteria in the biogeochemical flux of C in the Northern Adriatic Sea. The C transfer from the biotic versus the abiotic compartment seems to flow better in summer, particularly in area A.

Keywords

Aminopeptidase Activity Bacterial Growth Efficiency Electron Transport System Activity Mississippi River Plume Microbial Enzymatic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amon RMW, Benner R (1998) Seasonal patterns of bacterial abundance and production in the Mississippi River plume and their importance for the fate of enhanced primary production. Microb Ecol 35: 289–300PubMedCrossRefGoogle Scholar
  2. Artegiani A, Bregant D, Paschini E, Pinardi N, Raicich F, Russo A (1997a) The Adriatic Sea general circulation. I. Air-sea interactions and water mass structure. J Phys Oceanogr 27: 1497–1514Google Scholar
  3. Artegiani A, Bregant D, Paschini E, Pinardi N, Raicich F, Russo A (1997b) The Adriatic Sea general circulation. II. Baroclinic circulation structure. J Phys Oceanogr 27: 1515–1532CrossRefGoogle Scholar
  4. Azam F, Smith DC, Steward GF, Hagstrom A (1993) Bacteria-organic matter coupling and significance for carbon cycling. Microb Ecol 28: 167–179CrossRefGoogle Scholar
  5. Azzaro M (1997) Attività respiratoria (ETSa) e biomassa microbica (ATP) nel Mediterraneo Orientale. Tesi laurea, Univ Stud MessinaGoogle Scholar
  6. Caruso G, Leonardi M, Azzaro M, Zaccone R (1998) Microbial activities and organic matter turnover in an oligotrophic environment (Egadi Island). Biol Mar Mediterr 5: 27–34Google Scholar
  7. Del Giorgio PA, Cole JJ, Cimbleris A (1997) Respiration rates in bacteria exceed phytoplankton production systems. Nature 385: 148–151CrossRefGoogle Scholar
  8. Ducklow HW, Carlson CA (1992) Oceanic bacterial production. In: Marshall KC (ed) Advances in Microbial Ecology, vol 12. Plenum Press, New York, pp 113–181CrossRefGoogle Scholar
  9. Fuhrman JA, Ammerman JW, Azam F (1980) Bacterioplankton in the coastal euphotic zone: distribution, activity and possible relationships with phytoplankton. Mar Biol 60: 201–207CrossRefGoogle Scholar
  10. Heip CHR, Goosen NK, Herman PMJ, Kromkamp J, Middelburg JJ, Soetaert K (1995) Production and consumption of biological particles in temperate tidal estuaries. Oceanogr Mar Biol Annu Rev 33: 1–149Google Scholar
  11. Holligan PM, Harris RP, Newell RC, Harbour DS, Head RN, Linley EAS, Lucas MI, Tranter PRG, Weekly CM (1984) Vertical distribution and partitioning of organic carbon in mixed, frontal and stratified waters of die English channel. Mar Ecol Prog Ser 14: 111–127CrossRefGoogle Scholar
  12. Hoppe HG (1983) Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates. Mar Ecol Prog Ser 11: 299–308CrossRefGoogle Scholar
  13. Hoppe HG (1993) Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria. In: Kemp PF, Sherr BF, Sherr EB, Cole JJ (eds) Handbook of methods m aquatic microbial ecology. Lewis Publishers, Boca Raton, pp 423–431Google Scholar
  14. Hoppe HG, Kim S-J, Gocke K (1988) Microbial decomposition in aquatic environments: combined process of extracellular enzyme activity and substrate uptake. Appl Environ Microbiol 54: 784–790PubMedGoogle Scholar
  15. Karner M, Herndl GJ (1992) Extracellular enzymatic activity and secondary production in free-living and marine snow associated bacteria. Mar Biol 113: 341–347Google Scholar
  16. Karner M, Kuks D, Herndl GJ (1992) bacterial activity along a trophic gradient. Microb Ecol 24: 243–257CrossRefGoogle Scholar
  17. Karner M, Rassoulzadegan F (1995) Extracellular enzyme activity: indications for high short-term variability in a coastal marine ecosystem. Microb Ecol 30: 143–156Google Scholar
  18. La Ferla R, Azzaro M, Chiodo G (1996) Microbial respiratory activity in the euphotic zone of the Mediterranean Sea. Microbiologica 19: 243–250PubMedGoogle Scholar
  19. La Ferla R, Azzaro M, Chiodo G (1999) Microbial respiratory activity and CO2 production rates in the Otranto Straits (Mediterranean Sea). Aquat Ecol 1: 1–9Google Scholar
  20. La Ferla R, Bacci C, Chiodo G, Parrino S, Zoppini A (1998) Stime di abbondanza e biovolume batterioplanctonico nell’Adriatico Settentrionale: confronto tra AO e DAPI. Biol Mar Mediterr 5(1): 750–753Google Scholar
  21. Martinez R, Arnone RA, Velasquez Z (1990) Chlorophyll a and respiratory electron transport activity in microplankton from surface waters of the Western Mediterranean. J Geophys Res 95: 1515–1522CrossRefGoogle Scholar
  22. Michal G (1983) Determination of Michaelis constants and inhibitor constants. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 3rd ed, vol 1. Verlag Chemie, Weinheim, pp 86–104Google Scholar
  23. Middelboe M, Sondergaard M, Letarte Y, Borch NH (1995) Attached and free-living bacteria: production and polymer hydrolysis during a diatom bloom. Microb Ecol 29: 231–248CrossRefGoogle Scholar
  24. Nausch M, Pollehne F, Kersten E (1998) Extracellular enzyme activities in relation to hydrodynamics in the pomeranian bight (Southern Baltic Sea). Microb Ecol 36: 251–258PubMedCrossRefGoogle Scholar
  25. Packard TT (1971) The measurement of respiratory electron transport activity in marine phytoplankton. J Mar Res 29: 235–244Google Scholar
  26. Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25: 943–94CrossRefGoogle Scholar
  27. Puddu A, La Ferla R, Allegra A, Bacci C, Lopez M, Oliva F, Pierotti C (1998) Seasonal and spatial distribution of bacterial production and biomass along a salinity gradient (Northern Adriatic Sea). Hydrobiologia 363: 271–282CrossRefGoogle Scholar
  28. Roszack DB, Colwell RR (1987) Metabolic activity of bacterial cells enumerated by direct viable count. Appl Environ Microbiol 53: 2889–2893Google Scholar
  29. Smith DC, Simon M, Alldredge AL, Azam F (1992) Intense hydrolytic enzyme activity on marine aggregates and implications for rapid partide dissolution. Nature 359: 139–142CrossRefGoogle Scholar
  30. Smith DC, Steward GF, Long RA, Azam F (1995) Bacterial mediation of carbon fluxes during a diatom bloom in a mesocosm. Deep-Sea Res 42: 75–97Google Scholar
  31. Unanue M, Ayo B, Azùa I, Barcina I, Iriberri J (1992) Temporal variability of attached and free living bacteria in coastal waters. Microb Ecol 23: 27–39.CrossRefGoogle Scholar
  32. Unanue M, Azùa I, Arrieta JM, Labirua-Iturburu A, Egea L, Iriberri J (1998) Bacterial colonization and ectoenzymatic activity in phytoplankton-derived model particles: cleavage of peptides and uptake of amino acid. Microb Ecol 35: 136–146PubMedCrossRefGoogle Scholar
  33. Vosjan JH, Nieuwland G (1987) Microbial biomass and respiratory activity in the surface waters of the east Banda Sea and Northwest Arafura Sea (Indonesia) at the time of the Southeast monsoon. Limnol Oceanogr 32(3): 767–775CrossRefGoogle Scholar
  34. Zaccone R, Caruso G (1996) Stima dell’attività proteasica batterica in Adriatico Settentrionde mediante substrati fluorogenici. SiTE Atti 17: 87–90Google Scholar
  35. Zaccone R, Caruso G, Calì C, Scarfò R (1998) Primi dati sulla caratterizzazione microbiologica delle acque dell’Adriatico Settentrionale. In: Atti XII Congr AIOL vol II, pp 487–497Google Scholar
  36. Zaccone R, La Ferla R, Caruso G, Azzaro M (1999) Attività proteasica e respiratoria microbica in due aree dell’Adriatico Settentrionale. In: Atti XIII Congr AIOL, Portonovo Ancona 28–30 Settembre 1998, 13(1): 33–44Google Scholar
  37. Zweifel UL, Wikner J, Hagstrom A (1995) Dynamic of dissolved organic carbon in a coastal ecosystem. Limnol Oceanogr 40(2): 299–305CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Italia 2001

Authors and Affiliations

  • R. La Ferla
    • 1
  • R. Zaccone
    • 1
  • G. Caruso
    • 1
  • M. Azzaro
    • 1
  1. 1.Istituto Sperimentale TalassograficoCNRMessinaItaly

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