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Biodiversity at Ecogenetic Level in Three Species of Beach Fleas

  • L. Zane
  • E. Angelini
  • N. Longo
  • S. Marcato
  • P. M. Bisol

Abstract

Patterns of fine-scale niche diversification were investigated in three co-generic species of littoral amphipods. Population samples of Orchestia montagui, O. gammarella and O. mediterrmea were collected from the same sampling site, in the Venice Lagoon, and analysed by means of protein electrophoresis and RAPD techniques. The three species exhibited significant differences at the protein and the nuclear DNA level. Specific markers were identified that allow the correct species assignment of females, that are morphologically indistinguishable. Two species, O.montagui, that occurs under vegetal debris, and O. mediterranea, found on muddy infra-littoral substrates, were further investigated with regard to the resistance to high temperature and to glucose-phosphate isomerase (GPI) biochemical activity. The two species were found to have different resistance to high temperatures, with O. montagui showing the higher survival rate. The thermal stability of the common GPI genotype and the biochemical activity of this enzyme was higher in O. montagui. Our results strongly suggest that a relationship exists between habitat preference and metabolic properties that can explain the competition avoidance in these animals.

Keywords

Venice Lagoon Decamer Primer Gill Area World Conservation Monitoring Competition Avoidance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Battaglia B, Bisol PM (1988) Environmental factors, genetic differentiation, and adaptive strategies in marine animals. In: Rotschild BJ (ed) Toward a theory on biological-physical interactions in the world ocean. Kluwer Academic Publishers, New York, pp 393–410CrossRefGoogle Scholar
  2. Bellan-Santini D, Karaman G, Krapp-Schickel G, Ledoyer M, Ruffo S (1993). The Amphipoda of the Mediterranean. Part 3. Gammaridea (Meliipidae to Talitridae), Ingolfiellidea, Caprellidea. In: Ruffo S (ed) The Amphipoda of the Mediterranean. Mem Inst Oceanogr Monaco 13, Principauté de Monaco, pp 742–753Google Scholar
  3. Biasiolo A, Bisol PM, Battaglia B (1989) Indagine sui polimorfismi proteici in Anfipodi del genere Orchestia. I. Orchestia mediterranea A. Costa della laguna di Venezia. Rend Accad Naz Lincei 73: 299–305Google Scholar
  4. Bousfield EL (1983). An updated phyletic classification and paleohistory of the Amphipoda. In: Schram FR (ed) Crustacean phylogeny. Balkema, Rotterdam, pp 257–277Google Scholar
  5. Bousfield EL (1984) Recent advances in the systematics and biogeography of landhoppers (Amphipoda; Talitridae) of the Indo-Pacific Region. In: Radovsky FJ, Raven PH, Sohmer SH (eds) Biogeography of the tropical Pacific. Bishop Mus Spec Publ 72: 171–210Google Scholar
  6. Castiglione S, Wang G, Damiani G, Bandi C, Bisoffi S, Sala F (1993) RAPD fingerprints for identification and for taxonomic studies of elite poplar (Populus spp.) clones. Theor Appl Gene 87: 54–59CrossRefGoogle Scholar
  7. De Matthaeis E, Cobolli M, Mattoccia M, Saccoccio P, Scapini F (1994) Genetic divergence between natural populations of Mediterranean sandhoppers (Crustacea, Amphipoda) In: Beaumont AR (ed) Genetics and evolution of aquatic organisms. Chapmann and Hall, London, pp 15–29Google Scholar
  8. Frankham R (1995) Conservation genetics. Annu Rev Gene 29: 305–327CrossRefGoogle Scholar
  9. Grunbaun BW (1981) Handbook for forensic individualization of human blood and bloodstains. Sartorius GmbH, Göttingen, GermanyGoogle Scholar
  10. Lavie B, Nevo E (1982) Heavy metal selection of phosphoglucose isomerase allozymes in marine gastropods. Mar Biol 71: 17–22CrossRefGoogle Scholar
  11. Louis M (1977) Etude des populations de Talitridae des étangs littoraux Mediterranéens. I. Identification des cohortes, cycles et fécondité. Bull Ecol 8: 75–86Google Scholar
  12. Marsden ID (1984) Effects of submersion on the oxygen consumption of the estuarine sandhopper Transorchestia chiliensis (Milne-Edwards 1840). J Exp Mar Biol Ecol 79: 263–276CrossRefGoogle Scholar
  13. Moore PG, Francis CH (1985) On the water relations and osmoregulation of the beach-hopper Orchestia gammarellus (Pallas) (Crustacea: Amphipoda). J Exp Mar Biol Ecol 94: 131–150CrossRefGoogle Scholar
  14. Moore PG, Taylor AC (1984) Gill area relationships in an ecological series of gammaridean amphipods (Crustacea). J Exp Mar Biol Ecol 74: 179–186CrossRefGoogle Scholar
  15. Morritt D (1987) Evaporative water loss under desiccation stress in semiterrestrial and terrestrial amphipods (Crustacea: Amphipoda: Talitridae). J Exp Mar Biol Ecol 111: 145–157CrossRefGoogle Scholar
  16. Morritt D (1988) Osmoregulation in littoral and terrestrial talitroidean amphipods (Crustacea) from Britain. J Exp Mar Biol Ecol 123: 77–94CrossRefGoogle Scholar
  17. Nei M (1978) Estimation of average heterozigosity and genetic distance from a small number of individuals. Genetics 89: 583–590PubMedGoogle Scholar
  18. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
  19. OECD (1996) Saving biological diversity. (Economic incentives) ParisGoogle Scholar
  20. Pardi L, Ercolini A (1986) Zonal recovery mechanisms in talitrid crustaceans. Boll Zool 53: 139–160CrossRefGoogle Scholar
  21. Patarnello T, Bisol PM, Battaglia B (1989) Studies on differential fitness of GPI genotypes with regard to temperature in Gammarus insensibilis (Crustacea: Miphipoda). Mar Biol 102: 355–359CrossRefGoogle Scholar
  22. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Lab Press, Cold Spring Harbor, New YorkGoogle Scholar
  23. Shihab AF, Heath DJ (1987) Components of fitness and the GPI polymorphism in the freshwater isopod Asellus aquaticus (L.). 2. Zygotic selection. Heredity 58: 289–295CrossRefGoogle Scholar
  24. Swofford DL, Selander RB (1989) BIOSYS-1: a computer programme for the analysis of allelic variation in population genetics and biochemical systematics. Ill Nat Hist Surv, Ill, USAGoogle Scholar
  25. Taylor AC, Spicer JI (1986) Oxigen-transporting properties of the blood of two semi-terrestrial amphipods, Orchestia gammarellus (Pallas) and O. mediterranea (Costa). J Exp Mar Biol Ecol 97: 135–150CrossRefGoogle Scholar
  26. Watt WB (1992) Eggs, enzymes, and evolution-natural genetic variants change insect fecundity. Proc Nati Acad Sci USA 89: 10608–10612CrossRefGoogle Scholar
  27. Wildish DJ (1988) Ecology and natural history of aquatic Talitroidea. Can J Zool 66: 2340–2359CrossRefGoogle Scholar
  28. Williams JFK, Kubelik AR, Livak KJ, Rafalsky JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Aids Res 18: 6531–6535CrossRefGoogle Scholar
  29. World Conservation Monitoring Centre (1992) Global biodiversity: status of the Earth living resources. Chapman and Hall, LondonGoogle Scholar

Copyright information

© Springer-Verlag Italia 2001

Authors and Affiliations

  • L. Zane
    • 1
  • E. Angelini
    • 1
  • N. Longo
    • 1
  • S. Marcato
    • 1
  • P. M. Bisol
    • 1
  1. 1.Dipartimento di BiologiaUniversità degli Studi di PadovaPadovaItaly

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